HPTLC Analysis, Antioxidant and Antidiabetic activities of Ethanol Extract of Moss Fissidens grandiflora

Abstract

The phytochemical composition, antioxidant and antidiabetic profile of ethanol extract of Fissidens grandiflora was investigated for the first time. The phytochemical analysis of Fissidens grandiflora confirmed the presence of flavonoids, phenolics, steroids and tannins. Additionally, Fissidens grandiflora contained a high amount of total flavonoids of 101.40±0.38 mg Q/g. The high-performance thin-layer chromatography analysis of Fissidens grandiflora identified 3 unknown components with different R f values and area percentage. Fissidens grandiflora showed better inhibition of superoxide free radicals, 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radicals and ferric ions with IC 50 values of 81.0, 85.0 and 92.6 µg/ml, respectively. Additionally, Fissidens grandiflora depicted a prominent inhibitory profile against α-glucosidase and pancreatic α-amylase with IC 50 values of 150.0 and 200.0 µg/ml, respectively. To conclude, the proposed mechanism of action of moss Fissidens grandiflora could be by inhibiting free radicals and particularly digestive enzymes.

Alpha-Amylase Inhibitory and Antioxidant Activities in Aqueous Acetone Extract and its Fractions from Ampelocissus martini Root

“…Several methods such as DPPH, ABTS, CUPRAC and FRAP were performed to evaluate the antioxidant activity of substances by the reduction of certain compounds including metals and radicals because they are simple and widely used techniques [10,13] . AAF showed higher antioxidant activity compared to all samples in all assays.…”

“…Acarbose, inhibitor of α-amylase has been used for the management of hyperglycemia and type 2 diabetes, but with undesirable side effects including diarrhea and flatulence [7] . Finding natural and safer enzyme inhibitors with strong antioxidant activity has been proposed for the treatment of diabetes since it can control hyperglycemia and diabetes complications caused from oxidative stress [8][9][10] .…”

Protective effect of Vitex altissima L.f. bark extract on cisplatin-induced renal injury in Wistar rats

“…By employing the 1,1-diphenyl-2-picrylhydrazyl (DPPH, Sigma Aldrich Co., USA) assay (28) in triplicate, Va were evaluated for antioxidant activity. To the known concentrations of Va added 0.004% DPPH, and incubated for 30 min at 37 ˚C.…”

Bio-Assay Guided Identification of Antioxidant Fractions from Methanolic Extract of Polyalthia longifolia Seeds and their Hepatoprotective Effects against Ethanol Induced Oxidative Stress

“…Using column chromatography of mesh size 100-200, PLS (20 g) was fractionated [22] using a hexane/ ethyl acetate solvent system (step gradient flow from 100:0, 95:5, 90:10, …, 5:95, 0:100), which yielded five fractions (F1 (6 g), F2 (5 g), F3 (3 g), F4 (4 g) and F5 (2 g)). These fractions were subjected to antioxidant activity [23,24] (as per the procedure mentioned below) and noticed that only two of them, namely F3 (3 g) and F5 (2 g), were biologically more active (Table 1). In vitro antioxidant activity:…”

“…By employing the DPPH (Sigma Aldrich Co., USA) assay [23,24] in triplicate, the F1-5 and PLS were evaluated for antioxidant activity. To the known concentrations of F1-5 and PLS, added 0.004 % DPPH and incubated for 30 min at 37.…”