HPTLC Analysis of hydro alcoholic extracts of Clerodendrum viscosum V. leaves and Macrotyloma uniflorum L. seeds

Abstract

The objective of this study was to perform the HPTLC analysis of hydro alcoholic extracts of Clerodendron viscosum V. leaves & Macrotyloma uniflorum L. seeds. HPTLC is a powerful analytical tool in the identification of chemical constituents in plant extracts. Leaves of Clerodendron viscosum V. and seeds of Macrotyloma uniflorum L. are having numerous traditional usages ranging from wound healing to the treatment of tumors. Hydro alcoholic extracts of Clerodendron viscosum V. leaves & Macrotyloma uniflorum L. seeds were obtained using cold maceration. The obtained extracts were subjected to HPTLC analysis by using the instrument “CAMAG Linomat 5”. Quercetin, Rutin and Gallic acid were used as standards; Toluene: Ethyl Acetate: Formic Acid: Methanol (3:6:1.6:0.4) solution was used as a mobile phase. 10 µL of plant extracts and 5 µL of standard solutions were applied during the analysis. Both the plant extracts have shown the presence of Quercetin as one of the major constituent which is having numerous therapeutic properties. Extract of Macrotyloma uniflorum L. seeds has shown the presence of Quercetin with 0.91 Rf, 19.93 % Area, and 3655.7 Area; whereas the Clerodendron viscosum V. leaves extract has shown the presence of Quercetin with 0.90 Rf, 19.17 % Area, and 1685.9 Area.
 Keywords: HPTLC; Clerodendrum viscosum; Macrotyloma uniflorum; Quercetin