HPTLC Assay of Thymoquinone in Black Seed and Black Seed Oil (Nigella Sativa Linn) and Identification of Thymoquinone Conversion With Uv-Vis

Belete, Yehualashet; Dagne, Ermias

doi:10.22270/jddt.v4i4.895

Cited by 16 publications

(19 citation statements)

References 11 publications

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“…Evidence-Based Complementary and Alternative Medicine higher than 1% recorded in previous studies [32]. is amount was considerably higher than assayed TQ.…”

Section: Determination Of Uv λ Maxcontrasting

confidence: 53%

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HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

Habib

Choudhry

2021

Evidence-Based Complementary and Alternative Medicine

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The medicinal importance of Nigella sativa seeds for treating various ailments is portrayed by its traditional uses. Owing to its immense pharmacological importance, the thymoquinone phytoconstituent of N. sativa can prove beneficial for the South Asian countries including Pakistan, where this seed is commonly produced and healthcare facilities are limited. In this study, the antibacterial activity of various extracts of N. sativa seeds, extracted thymoquinone, and oil samples have been investigated against Bacillus subtilis and Bacillus licheniformis using well and disc diffusion assay. The inhibition zones ranged between 7 and 44 mm against both the bacterial strains by well diffusion assay, while disc diffusion assay provided inhibition zones in the range of 7–23 mm. Commercial and local Kalonji oil samples were included in the study. Oil samples dissolved in methanol showed increased inhibition of bacteria. However, the extracted thymoquinone showed highest antibacterial activity. Medicine formulated using thymoquinone will prove to be an herbal alternate against the resistant microbiota associated with bacterial infections. Antibacterial activity against some Bacillus species will help signify the effect on normal gut flora when oral therapy is followed. Trying different extraction protocols can help increase extraction efficiency. Study on extraction of thymoquinone in local produce of black seed can be fruitful for conducting the stability studies and can help to gain maximum benefits from the bioactives. The crude extracts from 10 g of these seeds were subjected to preliminary phytochemical investigation. Results showed that although methanol extract had the presence of maximum phytochemicals, hexane extract was the most potent in terms of antibacterial activity. Thymoquinone, a therapeutically important bioactive in N. sativa seed, was extracted employing both solvents. TLC assay and UV spectroscopy were used for its qualitative assessment, while HPLC-UV quantification showed that 250 mg/mL of methanol extract had 368.3 μg/mL thymoquinone, while its successive extraction yielded 32.94 μg/mL thymoquinone.

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“…Evidence-Based Complementary and Alternative Medicine higher than 1% recorded in previous studies [32]. is amount was considerably higher than assayed TQ.…”

Section: Determination Of Uv λ Maxcontrasting

confidence: 53%

“…The filtered marc was again soaked in 70 mL of methanol (80%) and shaking was done for 4 hours followed by filtration. The combined filtrates were diluted to have 50% methanol concentration [ 32 ]. Solvent extraction was done using chloroform, using multiple batch extraction.…”

Section: Microbiological Assessment For Antibacterial Activitymentioning

confidence: 99%

HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

Habib

Choudhry

2021

Evidence-Based Complementary and Alternative Medicine

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“…The present results are also in virtuous agreement with the previous finding of Belete and Dagne, [30], where thymoquinone was analyzed using UV-Visible, HPTLC and NMR techniques with maximum absorbance at 254nm. Further, RP-HPLC analysis of thymoquinone showed maximum percent of thymoquinone (0.108±0.0007%), in the NSE treated with 0µM concentration of sodium azide while, minimum percent of thymoquinone (0.035±0.003%) was observed at 100µM sodium azide treated NSE.…”

Section: Resultssupporting

confidence: 93%

Potential in Vitro Elicitation of Secondary Metabolite Using Sodium Azide in Tissue Culture of <em>Nigella sativa</em> and Its Eeffect on DNA Damage Inhibition

Ms¹,

Iqbal²,

Hashem³

et al. 2020

Preprint

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Nigella sativa (NS) is an effective medicinal plant possessing noteworthy antioxidant property. In NS, there are more than hundred phyto-chemicals reported, out of which thymoquinone is the utmost active phyto-constituent having sturdy antioxidative property. Thymoquinone is a cyclicdione, when reacts with sodium azide, converts into α-azido ketones i.e its analogs which are handy with extensive range of reactions. Sodium azide induces stress in plants thereby, modulating the antioxidant system. The present investigation was planned to elucidate the effect of sodium azide at different concentrations (5µM, 10µM, 20µM, 50µM, 100µM and 200µM) on its secondary metabolites (mainly thymoquinone) in NS callus culture extract (NSE). The results showed sodium azide effect on thymoquinone content and a concentration dependent boost in antioxidant property. It was also observed that thymoquinone content and percent yield (analyzed by RP-HPLC; Reverse Phase- High Performance Liquid Chromatography) were minimum (0.033±0.006% and 0.420±0.045%, respectively) at 200 µM sodium azide used. Whereas, antioxidant activity (analyzed by DPPH; 2, 2-diphenyl-1-picrylhydrazyl) was found to be maximum (3.873±0.402%) at same dose. Further, analysis was done for inhibition of oxidative DNA damage at different concentrations of sodium azide on NSE, maximum inhibition of DNA damage (0.243±0.017%) was found at 200 µM concentration of sodium azide. When correlated, strong positive correlation was observed between percent yield and percent thymoquinone, antioxidant property and inhibition of DNA damage. Whereas, strong negative correlation was observed between percent yield and antioxidant property, percent thymoquinone and antioxidant property, percent thymoquinone and inhibition of DNA damage. The findings evidently point out that the content of thymoquinone, antioxidant property and inhibition of DNA damage was affected by sodium azide.

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“…Ultraviolet-visible spectroscopy is a characterizing tool to ascertain the optical behaviour of a material. The absorbance spectrum of MNP [fig 1(UV-Vis analysis)] does not show any sharp peak in its spectrum whereas MC, MCP, MCT and MCPT samples exhibit a prominent peak at 423 nm, 343 nm, 430 http://sciforum.net/conference/mol2net-06 nm and 344 nm respectively [35,36]. There is a hump at 425 nm in MCP and MCPT UV spectra due to presence Curcumin [37]…”

Section: Mnpsmentioning

confidence: 99%

Facile Synthesis of Natural Therapeutics Encapsulated Biopolymeric Okra Mucilage Nanoparticles as Dual ameliorative agent

Pal

2020

Proceedings of MOL2NET 2020, International Conference on Multidisciplinary Sciences, 6th Edition

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Graphical AbstractAbstract.Among the different types of biomaterials, natural excipients Okra mucilage (MNP) is economic and has the potential for controlled drug delivery. We have synthesized MNPs by co-precipitation method and characterized them by XRD, FESEM, FTIR, UV-Vis spectra and DLS. Despite their potential anti-cancer activity, solubility of curcumin, piperine, thymoquinone is very low rendering its limit in application. We have used MNPs where the natural compounds like MOL2NET, 2020, 6, ISSN: 2624-5078 2 http://sciforum.net/conference/mol2net-06 curcumin, piperine, thymoquinone can be loaded comfortably and thereby increases its bioavailability. The antibacterial activity of these nanoparticles were evaluated against pathogenic bacterial strains. The cytotoxicity of curcumin ,piperine, thymoquinone encapsulated MNPs was evaluated on triple negative breast cancer cell lines. They were found to induce apoptosis by perturbing the mitochondrial membrane potential. Folic acid was conjugated to curcumin, piperine, thymoquinone encapsulated MNPs, for delivering it specifically to the breast cancer cells. The antimicrobial and anticancer potential of conjugated and non conjugated MNP were assessed by various in vitro cellular assays. Our present study confirms that these functionacan be used as a dual therapeutic option for combating pathogenic microbial strains and triple negative breast cancer cell.

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HPTLC Assay of Thymoquinone in Black Seed and Black Seed Oil (Nigella Sativa Linn) and Identification of Thymoquinone Conversion With Uv-Vis

Cited by 16 publications

References 11 publications

HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

Potential in Vitro Elicitation of Secondary Metabolite Using Sodium Azide in Tissue Culture of <em>Nigella sativa</em> and Its Eeffect on DNA Damage Inhibition

Facile Synthesis of Natural Therapeutics Encapsulated Biopolymeric Okra Mucilage Nanoparticles as Dual ameliorative agent

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