HPTLC-densitometric analysis of selected antidiabetic drugs in presence of their degradation products

Ahmed, Hytham M.; Omar, Mahmoud A.; Batakoushy, Hany A.; Hamid, Mohamed A. Abdel

doi:10.1016/j.microc.2019.104560

Cited by 11 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chromatographic techniques enable specialists to perform analysis of many active ingredients of pharmaceuticals in the presence of impurities, degradation products, and additives. [38][39][40] To date, no methods have been recorded for the concurrent analysis of the investigated mixture in the absence or presence of N-methyl-2pyrrolidone, polyoxy 35 castor oil, and sodium formaldehyde sulfoxylate additives. Hence, it was strongly recommended to achieve accurate, selective, and simple separation and quantification of the investigated mixture, especially in the presence of additives that interfere with the three drugs and hinder their selective determination if they are analyzed by UV spectroscopic techniques.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous analysis of oxytetracycline hydrochloride, lidocaine, and bromhexine hydrochloride in the presence of many interfering excipients

Mahmoud

Naguib

Abdelfatah

2021

Archiv der Pharmazie

View full text Add to dashboard Cite

A gradient elution high‐performance liquid chromatographic method with a diode array detector is introduced for the first time for the simultaneous estimation of three drugs, namely, oxytetracycline hydrochloride (OXT), lidocaine (LDC), and bromhexine hydrochloride (BRH), in a veterinary formulation (OxyClear® solution) that contains many interfering additives. The method used a C‐8 column. The chromatographic eluting solution included acidified water (0.1% trifluoroacetic acid in water) and acetonitrile at a 1‐ml/min flow rate and 254 nm as a nominated detection wavelength. The chromatographic process was assessed in terms of linearity, precision, accuracy, LOD, and LOQ. OXT, LDC, and BRH were linear in the range of 1–60, 5–100, and 1–60 μg/ml, respectively. The three drugs were determined successfully without the interference of three excipients having UV absorbances. Furthermore, the purities of the peaks of the three drugs were confirmed by comparing the UV spectra of investigated peaks to the UV reference spectra in Clarke's Analysis of Drugs and Poisons. The greenness value of the method was 0.69 with a faint green‐colored pictogram using the AGREE tool. These merits recommend the application of the planned method in QC laboratories for purity testing and concentration assays for the pure drugs and commercial formulations.

show abstract

Section: Resultsmentioning

confidence: 99%

Simultaneous analysis of oxytetracycline hydrochloride, lidocaine, and bromhexine hydrochloride in the presence of many interfering excipients

Mahmoud

Naguib

Abdelfatah

2021

Archiv der Pharmazie

View full text Add to dashboard Cite

show abstract

“…9-11 Literature survey disclosed numerous analytical approaches for the assessment of DPZ, SGT and MTH independently and in combined dosage form by means of UV-spectrophotometry, 12-16 HPLC [3][4][17][18][19][20][21] and HPTLC. 22,23 Moreover, few approaches were described for the assessment of DPZ, SGT and MTH in mixed formulation/synthetic mixture using HPLC 10 and HPTLC. 11 Nevertheless, the simultaneous estimation of DPZ, SGT and MTH in the ternary mixture has not been reported so far by anyone by UV-spectroscopic methods.…”

Section: Introductionmentioning

confidence: 99%

New Eco-friendly UV-spectroscopic Methods for Simultaneous Assessment of Dapagliflozin, Saxagliptin and Metformin in Ternary Mixture

Sen¹,

Jatu²,

Maheshwari³

et al. 2023

Ind. J. Pharm. Edu. Res.

View full text Add to dashboard Cite

Background and Aim: Qternmet XR ® consisting of dapagliflozin (10 mg), saxagliptin (5 mg), and metformin hydrochloride (1000 mg), is a fixed-dose combination (tablets) that improves glycemic control in individuals with diabetes mellitus (type 2). The projected work presents four spectrophotometric methods that are eco-friendly, quick, effortless, accurate and reproducible for the concurrent assessment of the ternary mixture. Materials and Methods: The 1 st approach works on the notion of unravelling pre-existing equations (simultaneous) by measuring absorbance at 223, 212 and 232.6 nm for dapagliflozin, saxagliptin and metformin hydrochloride, sequentially. The second method namely ratio difference spectroscopy works by evaluating the variation in amplitude at two dissimilar wavelengths in the ratio spectra. Whereas the derivative ratio spectrum zero-crossing approach (third approach) relied on the utilization of the derivative ratio signals at zero-crossing locations. The fourth approach is the double divisor-ratio spectra derivative approach in which the first derivative of ratio spectrum was acquired and the concentrations of all 3 drugs in their combination were quantified. Results and Discussion: All the three drugs exhibited excellent linear correlation in the concentration series of 2-10 µg/mL for simultaneous equation method and 0.5-10 µg/mL for all the other methods with an exceptional correlation coefficient value. Furthermore, the projected approaches were authenticated in line with ICH strategies and which displayed suitable precision, accuracy and sensitivity. Conclusion:The developed spectrophotometric approaches when compared to other analytical procedures are regarded to be more cost-effective because they do not require expensive solvents or sophisticated instruments. Therefore, the projected methods could be effectively employed for the concurrent assessment of dapagliflozin, saxagliptin and metformin hydrochloride in ternary mixture.

show abstract

“…UV spectrophotometry [10–14], HPLC [3, 15–20], and high‐performance thin layer chromatography (HPTLC) [21–25] are among the analytical approaches stated in the literature for quantifying the concentrations of DPZ and VGT, either separately or in blend with other substances, in bulk drug samples or pharmaceutical formulations. Among the various methods reported, Attimarad et al.…”

Section: Introductionmentioning

confidence: 99%

“…UV spectrophotometry [10][11][12][13][14], HPLC [3, [15][16][17][18][19][20], and high-performance thin layer chromatography (HPTLC) [21][22][23][24][25] are among the analytical approaches stated in the literature for quantifying the concentrations of DPZ and VGT, either separately or in blend with other substances, in bulk drug samples or pharmaceutical formulations. Among the various methods reported, Attimarad et al (2023) [10] presented a study where they simultaneously determined DPZ and VGT in a combined formulation using three distinct UV spectroscopic methods.…”

mentioning

confidence: 99%

Development and validation of high‐performance thin layer chromatographic method for concurrent estimation of dapagliflozin and vildagliptin in combined tablet

Sen,

Khatariya,

Sen

et al. 2023

Separation Science Plus

View full text Add to dashboard Cite

Enhanced glycemic regulation in individuals with diabetes mellitus can be achieved using a fixed‐dose combination of dapagliflozin (10 mg) and vildagliptin (100 mg) in tablet. The primary objective of this research was to develop and validate a high‐performance thin layer chromatographic methodology for accurately measuring the quantities of dapagliflozin and vildagliptin in combined tablet formulation. The methodology involved using aluminum plates layered with silica gel 60F254, and the solvent system comprised of acetonitrile, benzene, and glacial acetic acid (9:1:2 v/v/v). Densitograms were scanned at a wavelength of 210 nm. The linearity of the procedure was established in the series of 200–2500 ng/band for dapagliflozin and 2000–25000 ng/band for vildagliptin, with correlation coefficients (r2) of 0.9931 and 0.9954, correspondingly. The method demonstrated good sensitivity, with detection limits of 21.07 ng/band for dapagliflozin and 154.97 ng/band for vildagliptin; quantification limits of 63.84 ng/band for dapagliflozin and 469.60 ng/band for vildagliptin. The methodology was found to be precise (% relative standard deviation of peak area <2) and accurate (recovery between 97% and 103%). Proposed method was found to be superior and capable of overcoming the shortcomings of previously reported methods for the assessment of dapagliflozin and vildagliptin in combined formulation.

show abstract

HPTLC-densitometric analysis of selected antidiabetic drugs in presence of their degradation products

Cited by 11 publications

References 36 publications

Simultaneous analysis of oxytetracycline hydrochloride, lidocaine, and bromhexine hydrochloride in the presence of many interfering excipients

Simultaneous analysis of oxytetracycline hydrochloride, lidocaine, and bromhexine hydrochloride in the presence of many interfering excipients

New Eco-friendly UV-spectroscopic Methods for Simultaneous Assessment of Dapagliflozin, Saxagliptin and Metformin in Ternary Mixture

Development and validation of high‐performance thin layer chromatographic method for concurrent estimation of dapagliflozin and vildagliptin in combined tablet

Contact Info

Product

Resources

About