HPTLC Detection of<i>Carica papaya</i>Dried Seed (Adulterant) in Trikatu Based on Piperine

Singh, Arjun; Sharma, Himanshu; Narshimhaji, Ch. Venkata; Singh, Ranjit; Srikanth, N

doi:10.1080/22311866.2019.1605933

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…If maintained under the same conditions, HPTLC analysis shows that all the three batches of TC have an Rf value of 0.43, indicating the presence of Piperine ( 45 – 47 ). Piperine is an essential and major component of TC formulation and its presence in the sample suggests its standard quality.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental condition was maintained at 20 ± 2 • C. After derivatizing plates with anisaldehyde sulphuric acid reagent and photo documentation with CAMAG Reprostar 3 photo documentation platform at 550 nm, piperine could be detected. The solvent system consisting of tolueneethyl acetate-glacial acetic acid (8:2:0.1, v/v/v) was used to resolve piperine from the matrix of TC (45)(46)(47). Densitometric determinations were performed in fluorescence mode by CAMAG TLC scanner.…”

Section: Preparation and Physicochemical Analysis Of Testing Formulationmentioning

confidence: 99%

“…If maintained under the same conditions, HPTLC analysis shows that all the three batches of TC have an Rf value of 0.43, indicating the presence of Piperine (45)(46)(47). Piperine is .…”

Section: Physicochemical Analysismentioning

confidence: 99%

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Deciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and in vivo validation

et al. 2022

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Trikatu Churna (TC) comprising Zingiber officinale rhizome, Piper longum, and Piper nigrum fruit, is effective in treating liver diseases and has high nutraceutical values. However, the efficacy of TC in treating alcoholic liver disease (ALD) and its mechanism remain largely unknown. This study evaluated the hepatoprotective effects of different doses of TC as well as to identify the bioactive components and determine their mechanism of action against ethanol-induced ALD. A compound-target network analysis model of TC was established to identify its potential bioactive compounds and pathways that might regulate its hepatoprotective effects. Further, in-vivo studies were performed to validate the potential of TC (200 mg/kg and 400 mg/kg b.w.) in the treatment and management of ALD. The study revealed that both the dosages of TC demonstrate significant (p > 0.0001) hepatoprotective effects by improving body weight, total bilirubin, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), serum alkaline phosphate (ALP), total cholesterol, total protein, globulin, albumin, and liver morphology. The High-performance thin-layer chromatography (HPTLC) fingerprinting of TC showed the presence of piperine. Network pharmacology identifies the role of TC in regulating various signaling processes including Advanced glycation end products-receptor for advanced glycation end products (AGE-RAGE), Hypoxia-inducible factors (HIF-1), Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-Kappa B), and Phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling to exert its anti-inflammatory, antioxidant and anti-apoptotic role in managing ALD. Based on the bioinformatics analysis, some of the key targets of TC were found to be Prostaglandin-Endoperoxide Synthase 2 (PTGS2) or Cyclooxygenase-2 (COX-2), Sirtuin 1 (SRT1), and caspase-3. These effects may serve as a novel therapeutic option for the treatment of ALD. These preclinical validation studies for the ethnopharmacological potential of TC in ALD treatment further paved the way for researchers to perform next-level translational and clinical studies. Further, in-depth experimental studies for the validation of these bioinformatics-based results will give a clearer picture of mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation and Physicochemical Analysis Of Testing Formulationmentioning

confidence: 99%

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Deciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and in vivo validation

et al. 2022

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Tissue-specific variations of piperine in ten populations of Piper longum L.: bioactivities and toxicological profile

Biswas,

Pandey,

Shekhawat

et al. 2024

Sci Rep

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P. longum L., one of the most significant species of the genus Piperaceae, is most frequently employed in Indian-Ayurvedic and other traditional medicinal-systems for treating a variety of illnesses. The alkaloid piperine, is the key phytoconstituent of the plant, primarily responsible for its’ pharmacological-impacts. The aim of the study is to analyse the intra-specific variation in piperine content among different chemotypes (PL1 to PL 30) and identify high piperine yielding chemotype (elite-chemotype) collected from 10 different geographical regions of West Bengal by validated HPTLC chromatography method. The study also focused on the pharmacological-screening to better understand the antioxidant activity of the methanol extracts of P. longum by DPPH and ABTS radical-scavenging activity and genotoxic activity by Allium cepa root tip assay. It was found that the P. longum fruit chemotypes contain high amount piperine (highest 16.362 mg/g in chemotype PL9) than the stem and leaf chemotypes. Both DPPH and ABTS antioxidant assays revealed that P. longum showed moderate radical-scavenging activity and the highest activity was found in PL9 (fruit) chemotype with IC50 values of 124.2 $$\pm 0.97$$ ± 0.97 and 104 $$\pm 0.78$$ ± 0.78 µg/ml respectively. The A. cepa root tip assay showed no such significant genotoxic-effect and change in mitotic-index. The quick, reproducible, and validated HPTLC approach offers a useful tool for determining quantitative variations of piperine among P. longum chemotypes from different geographical-regions and also according to the different tissues and choose elite genotypes with high piperine production for continued propagation and commercialization for the pharmaceutical sector. Additionally, the plant's in-vitro antioxidant property and lack of genotoxicity directly supports its’ widespread and long history of use as a medicinal and culinary plant.

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Chemical Profiling of the Leaf Essential Oils of Cinnamomum Species Used as a Spice in Southern India

Sriramavaratharajan

Murugan²

2020

Journal of Biologically Active Products from Nature

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HPTLC Detection ofCarica papayaDried Seed (Adulterant) in Trikatu Based on Piperine

Cited by 3 publications

References 7 publications

Deciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and in vivo validation

Deciphering the impact and mechanism of Trikatu, a spices-based formulation on alcoholic liver disease employing network pharmacology analysis and in vivo validation

Tissue-specific variations of piperine in ten populations of Piper longum L.: bioactivities and toxicological profile

Chemical Profiling of the Leaf Essential Oils of Cinnamomum Species Used as a Spice in Southern India

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