HPTLC-direct bioautography-guided isolation of isogeranic acid as the main antibacterial constituent of Artemisia santonicum essential oil

Abstract

This study was performed to determine the main antibacterial compounds of the essential oil (ЕО) of saltmarsh plant Artemisia santonicum (Asteraceae). The combination of HPTLC and direct bioautography was used for the activity guided isolation of isogeranic acid as the main antibacterial constituent with remarkable antimicrobial activity, although it was the minor component of the EO, present only in 0.2 %, as calculated from GC/FID. Its structure was determined by 1D-and 2D-NMR and GC-MS techniques. Antibacterial activity of isogeranic acid against all tested bacteria was significantly higher than EO and even than both controls streptomycin and ampicillin. In further investigation of antibiofilm and antiquorum sensing activity EO exhibited the best inhibition of the biofilm formation at 1/8 minimal inhibitory concentration (MIC) and isogeranic acid at 1/2 MIC. Both EO and isogeranic acid possessed pyocyanin inhibitory activity showing the reduction of pigment at 60.6 and 62.8 %, respectively, at 1/2 MIC concentrations.

HPTLC-based metabolomics for the investigation of metabolic changes during plant development: The case study of Artemisia annua

“…All NMR spectra ( 1 H, 13 C, COSY, HSQC and HMBC) were recorded in CDCl3 (with TMS for referencing) on a Bruker Avance III 500 NMR spectrometer operating at a proton NMR frequency of 500.26 MHz, equipped with a 5 mm BBI probehead. GC/MS analyses were performed according to Stankovic et al, (2019) 8 with some modifications of the temperature program and split ratio. The oven temperature was programmed from 60 to 240 °C at 3 °C/min, then from 240 to 310 °C at 35 °C/min, and then held isothermally for 8 min.…”

Chicory Extracts and Sesquiterpene Lactones Show Potent Activity against Bacterial and Fungal Pathogens

“…The antimicrobial activity of the samples was also tested by analyzing the type of growth inhibition (biostatic or biocidal) using a modified microdilution method [ 40 ] and bioautography on TLC plates [ 41 ]. We tested S. aureus (oral isolate), S. aureus ATCC 11632, P. aeruginosa ATCC 27853, P. aeruginosa IBRS P001, and E. coli 25922.…”

“…In the modified bioautographic method [ 41 ], we applied 10 µL of Ci_SFE opt and Ci_SFE pur (2 mg/mL in 30% ethanol) to a Kieselgel 60 F254 TLC plate (Merck) and fractionated the duplicate samples using an 85:15 ( v / v ) mixture of dichloromethane and methanol as the mobile phase. The position of the first tape was 1.50 cm from the left and 1.00 cm from the bottom.…”

Artemisia santonicum L. and Artemisia lerchiana Web. Essential Oils and Exudates as Sources of Compounds with Pesticidal Action

“…The literature survey on the essential oil profile of A. santonicum showed significant variability in major components depending on the origin. Thus, eucalyptol (1,8-cineole), chrysanthenone, and cis-thujone were reported as the main components in the oil from a Serbian population [41]. At the same time, camphor was found to be the principal compound in the plant material from Turkish populations [32,35,42].…”