HPTLC Fingerprint Analysis of Plant Staminal Cells Products

Abstract

Utilization of natural products is radically changing. Changes were mainly due to the outcome in the market of a plethora of new food supplements, and in particular those generally named botanicals for their common plant origin. The validation of these novel products needs powerful analytical devices tailored for the study of herbal extracts in order to assess composition and face their natural complexity as a resource. The last item is important and crucial for the capacity and utility of the analytical results that means that each product should be analyzed with the right approach. Having in mind these arguments, we selected HPTLC as useful tool for the analysis of products based on plant staminal (stem) cells. Nowadays these products, generally named bud-derivatives, are waiting scientific validation to obtain their own place into food supplements regulation, after gained that in the market. Our analyses, based on HPTLC fingerprints, were able to show bud-derivatives complex compositions that resulted very similar, but also in part different, to those of the corresponding leaf hydro-alcoholic extract.

New solutions using natural products

“…A protein seems perfectly comfortable inside the cytoplasm, but outside, irreversible denaturation causes definitive degradation and consequent difficulty in understanding the protein’s functionality. In contrast, small molecules are more stable in any environment and their molecular structures at least can be determined by phytochemical analysis ( Nicoletti and Toniolo, 2012 ; Toniolo et al, 2014 ). However, in the metabolome we have hundreds of thousands of different constituents to be studied, and the classic approach to study the molecules one by one is impracticable, and other methods must be utilized.…”

Intelligent and Smart Packaging

“…In fact, the metabolomics approach allows obtaining the widest possible coverage, in terms of the type and number of analyzed compounds. The ingerprint by HPTLC method was successfully used to determine the herbal composition of neem studied product [62][63][64][65][66][67][68][69], evidencing the complexity in composition and the multiplicity of activities.…”