HPTLC fingerprinting, photopigments, and nutrient analysis of <i>Selinum tenuifolium</i> along the altitudinal gradient

Srivastava, Ravi Prakash; Dixit, Pooja; Singh, Lav; Nagpoore, Nem Kumar; Pandey, Shivaraman; Verma, Praveen Chandra; Rawat, Vivek; Saxena, Gauri

doi:10.1080/10826076.2020.1848861

Cited by 2 publications

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“…Selinum carvifolia plants have shown useful therapeutic effects, which are mostly dependent on the presence of phenols, flavonoids, and terpenoids. This is a perennial herb native to the high altitudes of Uttarakhand, Himachal Pradesh, and the neighboring regions of Indian Western Himalayas (38). This important species grows in humus-rich mountainous regions of the Himalayas between 2,200-4,000 m in states of Himachal Pradesh, Uttarakhand, and Sikkim in India and in neighboring countries such as Nepal and China (39).…”

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confidence: 99%

Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India

et al. 2023

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Antibiotic resistance poses a serious threat to public health, raising the number of diseases in the community. Recent research has shown that plant-derived phenolic compounds have strong antimicrobial, antifungal, and cytotoxic properties against a variety of microorganisms and work as great antioxidants in such treatments. The goal of the current work is to evaluate the anticancerous, antibacterial, antifungal, antioxidant, and cytotoxicity activities in the extracts of the different plant parts (leaves, stems, and roots) of S. carvifolia (L.) L. This is a medicinally important plant and has been used for different kinds of diseases and ailments such as hysteria and seizures. The phenolic compounds from the different plant parts were analyzed using HPLC and the following were found to be present: chlorogenic acid, gallic acid, rutin, syringic acid, vanillic acid, cinnamic acid, caffeic acid, and protocatechuic acid. Gallic acid was found to have the highest concentration (13.93 mg/g), while chlorogenic acid (0.25 mg/g) had the lowest. The maximum TPC value, which ranged from 33.79 to 57.95 mg GAE/g dry extract weight, was found in the stem. Root extract with 9.4 mg RE/g had the greatest TFC level. In the leaf and stem extracts, the RSC ranged from 0.747 mg/mL to 0.734 mg/1 mL GE/g dry extract weight, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to measure in vitro antioxidant activity. In a concentration-dependent way, promising antioxidant activity was reported. Moreover, 3,5-dinitrosalicylic acid (DNSA) and the Folin–Ciocalteu phenol reagent technique were used to determine reducing sugar content and total phenolic content, respectively. Antibacterial activity against eight strains (MIC: 250–1,000 μg/mL) was analyzed, and the stem extract exhibited maximum activity. Antifungal activity was also assessed, and potent activity was reported especially in the extract obtained from the stem. Cytotoxicity was evaluated using an MTT assay in the A549 cell line, where different doses (0.0625, 0.125, 0.25, 0.5, and 1 mg/mL) of leaf, root, and stem extracts were used. Treatment with these extracts reduced the cell viability, indicating that S. carvifolia may possess anticancer potential, which can be of great therapeutic value.

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Section: Introductionmentioning

confidence: 99%

Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India

et al. 2023

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Antioxidant and Antibacterial Properties of bio-pigment from Beta vulgaris

Patel,

Pithawala

2023

Biosci., Biotech. Res. Asia

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Plants are essential for sustaining life on our planet, providing a diverse range of species that possess therapeutic properties. With a growing awareness of health and well-being among consumers, there has been a rise in the popularity of natural colorants sourced from plant-based materials. In this context, the main objective of this research was to extract valuable natural pigments from Beta vulgaris (commonly known as beetroot), with the aim of increasing the availability of pigments from natural sources while simultaneously minimizing environmental and health risks. The crude extract of pigments was obtained using the maceration method during the extraction process. The crude extract was then purified using flash column chromatography with various solvents as the mobile phase. Preliminary phytochemical screening revealed the presence of active compounds like phenols, carbohydrates, glycosides, phytosterols, tannins, flavonoids, alkaloids, terpenoids, and saponins. High-Performance Thin-Layer Chromatography (HPTLC) was performed to analyze the betalain profile, showing distinct bands at specific Rf values. The specific functional groups were identified in the Fourier-Transform Infrared (FTIR) spectrum based on the presence of characteristic bands. Antibacterial activity testing demonstrated varying degrees of inhibition against test organisms like Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The evaluated plant pigments exhibited positive results in terms of antioxidant activity, exhibiting free radical scavenging capabilities. The aqueous methanol extract showed the highest extraction yield at 36.71%. The aqueous methanol extract of Beta vulgaris showed the maximum antibacterial activity at 20 mm against Bacillus cereus. The methanol extract and aqueous ethanol extract displayed the lowest IC50 values (45.56 µg/ml and 43.23 µg/ml, respectively), indicating their high antioxidant potential. These findings highlight the promising potential of Beta vulgaris as a valuable source of natural pigments with both antibacterial and antioxidant properties.

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HPTLC fingerprinting, photopigments, and nutrient analysis of Selinum tenuifolium along the altitudinal gradient

Cited by 2 publications

References 24 publications

Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India

Major phenolic compounds, antioxidant, antimicrobial, and cytotoxic activities of Selinum carvifolia (L.) collected from different altitudes in India

Antioxidant and Antibacterial Properties of bio-pigment from Beta vulgaris

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