HPTLC fluorescence enhancement of glutathione-SBD: Effect of cyclodextrins, triton and alkanes

Baeyens, W. R. G.; Ling, Bowen; Stragier, K.

doi:10.1007/bf02317911

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…The presence of cyclodextrins can dramaticaly enhance the fluorescence signal of complexed solutes (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36). The first application of cyclodextrins in this regard appears to have been reported by Kinoshita and co-workers for dansylated amino acids in a series of papers in the mid 70s (6,(19)(20)(21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the relative effectiveness of different water-soluble .beta.-cyclodextrin media to function as fluorescence enhancement agents

Frankewich¹,

Thimmaiah²,

Hinze³

1991

Anal. Chem.

110

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The effects of 8-cyclodextrln as well as four water-soluble 8-cyclodextrln systems, l.e. urea-solubllized 8-cyclodextrln, and the water-soluble derivatives, hydroxyethyl-8-cyclodextrln, 2-hydroxypropyl-8-cyclodextrln, and heptakls (2,6dl-0-methyl)-8-cyclodextrln, upon the fluorescence behavior of 14 dansylamlno acids and 33 organlc/pharmaceutlcal compounds were determined. In addition, fluorescence data on these solutes were obtained In the homogeneous solvents water and methanol. The use of the more water-soluble 8cyclodextrln systems typically resulted In greater fluorescence from these 47 compounds compared to that obtainable with native 8-cyclodextrln. It Is thought that the added fluorescence enhancements observed are due to the fact that a greater fraction of the solute molecules are complexed within the protective cyclodextrin cavity at the greater cyclodextrin concentrations obtainable with these water-soluble systems.The use of either 2-hydroxypropyl-or 2,6-di-0-methyl-8cyclodextrln generally resulted In the greatest fluorescence enhancement factors and increased the stability of some fluorescent systems. The former 8-cyclodextrln derivative offers an advantage compared to the rest In terms of ease of volume handllng/manipulatlon considerations due to Its lower solution viscosity. The results Indicate that If a 8-cyclodextrln-enhanced fluorescence assay Is being contemplated, use of 2-hydroxypropyl-8-cyclodextrin In lieu of native 8-cyclodextrln should be considered.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the relative effectiveness of different water-soluble .beta.-cyclodextrin media to function as fluorescence enhancement agents

Frankewich¹,

Thimmaiah²,

Hinze³

1991

Anal. Chem.

110

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Fluorescence in Organized Assemblies

Hinze¹

2000

Encyclopedia of Analytical Chemistry

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This article provides an overview of the general properties of organized assembly (ordered media) systems such as aqueous surfactant and bile salt micelles, lipid and surfactant vesicles (liposomes) and cyclodextrins (CDs) and summarizes their utilization to enhance the performance of analytical fluorescence measurements. In many instances, organic molecules and metal complex species, when included within a CD cavity or solubilized and bound to surfactant aggregates, exhibit enhanced fluorescence. This gives rise to improved detectability of such analytes. The altered microenvironment within the organized medium is capable of impeding the interfering action of other species (inorganic or organic) present in the sample matrix. This often can improve the selectivity of the analytical method. These benefits of improved sensitivity and selectivity arise from the compartmentalization, isolation and shielding of the excited singlet state of the guest analyte from quenching and nonradiative decay processes as well as prevent side reactions that otherwise can occur in bulk solution (or sample matrix). In addition, organic solvents or time‐consuming extraction steps can be avoided owing to the increased solubility of the nonpolar organic or inorganic reagents and/or analyte molecules in water in the presence of the organized medium; allowing for the use of an aqueous medium to perform the procedure. The possibility of conducting reactions and forming fluorescent organic or metal chelates in micellar (or other organized) media that are not observed in a bulk homogeneous solvent system serves to expand the scope of chemistries that one can consider using to design/develop new, unique and improved fluorescent assays. Numerous representative examples of fluorescent methods for determination of both organic and inorganic analytes are provided which serve to illustrate the advantages and benefits accrued from the use of the micelles, vesicles, liposomes or CDs in such procedures. Some experimental considerations and cautions to keep in mind when utilizing organized media are also delineated. An extensive reference section is provided so that the interested reader can easily refer to such for more detailed information on these systems and topics.

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Fluorescence in Organized Assemblies

Memon¹,

Balouch²,

Hinze³

2008

Encyclopedia of Analytical Chemistry

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This article provides an overview of the general properties of organized assembly (ordered media) systems, such as aqueous surfactant and bile salt micelles, lipid (liposomes) and surfactant vesicles, and cyclodextrins (CDs), and summarizes their utilization to enhance the performance of analytical fluorescence measurements. In many instances, organic molecules and metal complex species when included within a CD cavity or solubilized and bound to surfactant aggregates exhibit enhanced fluorescence, improving detectability of such analytes. The altered microenvironment within the organized medium can impede the interfering action of other species (inorganic or organic) present in the sample matrix, which often improves the selectivity of the analytical method. These benefits of improved sensitivity and selectivity arise from the compartmentalization, isolation, and shielding of the excited singlet state of the guest analyte from quenching and nonradiative decay processes, as well as preventing side reactions that otherwise can occur in bulk solution (or sample matrix). Organic solvents or time‐consuming extraction steps can also be avoided, owing to the increased solubility of nonpolar organic or inorganic reagents and/or analyte molecules in water in the presence of the organized medium, allowing the use of an aqueous medium to perform the procedure. The possibility of conducting reactions and forming fluorescent organic or metal chelates in micellar (or other organized) media that are not observed in a bulk homogeneous solvent system serves to expand the scope of possible chemistries for design/development of new, unique, and improved fluorescent assays. Examples of fluorescent methods for determination of both organic and inorganic analytes are provided, which serve to illustrate the advantages and benefits accrued from the use of the micelles, vesicles, liposomes, or CDs in such procedures, along with experimental considerations and cautions in utilizing organized media.

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HPTLC fluorescence enhancement of glutathione-SBD: Effect of cyclodextrins, triton and alkanes

Cited by 3 publications

References 21 publications

Evaluation of the relative effectiveness of different water-soluble .beta.-cyclodextrin media to function as fluorescence enhancement agents

Evaluation of the relative effectiveness of different water-soluble .beta.-cyclodextrin media to function as fluorescence enhancement agents

Fluorescence in Organized Assemblies

Fluorescence in Organized Assemblies

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