HPTLC-guided flash chromatographic isolation and spectroscopic identification of bioactive compounds from olive flowers

Agatonovic-Kustrin, Snezana; Wong, Sheryn; Dolzhenko, Anton V.; Gegechkori, Vladimir; Morton, David W.

doi:10.1016/j.chroma.2024.465310

Journal of Chromatography A

2024

DOI: 10.1016/j.chroma.2024.465310

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HPTLC-guided flash chromatographic isolation and spectroscopic identification of bioactive compounds from olive flowers

Snezana Agatonovic-Kustrin,

Sheryn Wong,

Anton V. Dolzhenko

et al.

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Review on Method Development and Validation of Metformin, Empagliflozin and Glimepiride Using UPLC

Daakshayani,

Neha,

Sravani

et al. 2024

International Journal of Innovative Science and Research Techno

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UPLC, also known as ultra performance liquid chromatography, was developed by Waters in 2004 and represents an advanced form of HPLC(1). This method has revolutionized liquid chromatography by catering to particles smaller than 2 μm, leading to enhanced sensitivity, speed, and resolution. Many experts believe that UPLC will eventually supplant traditional HPLC methods(2). The Van Deemter equation underpins this transformative process by establishing a connection between linear velocity and plate height.(1) Operating with UPLC on smaller particles necessitates a higher pressure limit compared to standard HPLC, which typically operates at 6000 psi. In comparison to HPLC, this method reduces mobile phase volume usage by at least 80% and shortens the runtime to approximately 1.5 minutes(3,4). UPLC, a modified version of HPLC, takes advantage of advancements in particle chemistry performance, system architecture, detector design, data processing, and control, resulting in significant enhancements in resolution, sensitivity, and efficiency(3). This also leads to quicker results and reduced solvent usage, making the technology more cost-effective and eco- friendly(4)

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Review on Method Development and Validation of Metformin, Empagliflozin and Glimepiride Using UPLC

Daakshayani,

Neha,

Sravani

et al. 2024

International Journal of Innovative Science and Research Techno

View full text Add to dashboard Cite

show abstract

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HPTLC-guided flash chromatographic isolation and spectroscopic identification of bioactive compounds from olive flowers

Cited by 1 publication

References 23 publications

Review on Method Development and Validation of Metformin, Empagliflozin and Glimepiride Using UPLC

Review on Method Development and Validation of Metformin, Empagliflozin and Glimepiride Using UPLC

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