HPTLC of phospholipid mixtures containing phosphatidylglycerol

Vaskovsky, V.E.; Terekhova, T. A.

doi:10.1002/jhrc.1240021107

Cited by 89 publications

(43 citation statements)

References 23 publications

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“…Lipids were extracted by a method modified from Bligh & Dyer (1959). Polar lipids were separated by two-dimensional micro-TLC in solvent systems described by Vaskovsky & Terekhova (1979). Lipids were detected by TLC using 10 % H 2 SO 4 in methanol with subsequent heating to 180 uC and using specific reagents for phospholipids (Vaskovsky et al, 1975) and amino-containing lipids (2 % ninhydrin in acetone) and Dragendorf's reagent for choline lipids.…”

Section: T (=Lmg 22531mentioning

confidence: 99%

Marinomonas pontica sp. nov., isolated from the Black Sea

Ivanova

Onyshchenko

Christen

et al. 2005

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-negative, polarly flagellated bacterium was isolated from a sea-water sample collected from the Karadag Natural Reserve of the Eastern Crimea and characterized to clarify its taxonomic position. 16S rRNA gene sequence-based phylogenetic analysis of this novel organism revealed Marinomonas vaga, Marinomonas communis, Marinomonas mediterranea, Marinomonas primoryensis and 'Marinomonas protea' as its closest relatives (similarity 95-97 %). The G+C content of the DNA was 46?5 mol%. The organism grew between 4 and 33 6C, tolerated 10 % NaCl, was slightly alkaliphilic and was not able to degrade starch, gelatin, agar or Tween 80. Phosphatidylethanolamine (53?4 %) and phosphatidylglycerol (46?6 %) were the predominant phospholipids. The major fatty acids were 16 : 0 (15?5 %), 16 : 1v7 (26?7 %) and 18 : 1v7 (47?1 %). The phylogenetic, genetic and physiological properties of the organism placed it within a novel species, proposed as Marinomonas pontica sp. nov., the type strain of which is 46-16 T (=LMG 22531 T =KMM 3492 T =UCM 11075 T ).

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Section: T (=Lmg 22531mentioning

confidence: 99%

Marinomonas pontica sp. nov., isolated from the Black Sea

Ivanova

Onyshchenko

Christen

et al. 2005

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…For polar lipid and fatty acid analyses, strains KMM 3891 T and KMM 3892 were cultivated on MA at 28 u C for 3 days and lipids were extracted using the chloroform/methanol extraction method of Bligh & Dyer (1959). Polar lipids were analysed as described by Vaskovsky & Terekhova (1979). Fatty acid methyl esters were obtained by alkaline methanolysis (15 % NaOH/methanol).…”

mentioning

confidence: 99%

Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

Romanenko

Tanaka

Frolova

2010

International Journal of Systematic and Evolutionary Microbiology

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Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).

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“…Polar lipids were analysed as described by Vaskovsky & Terekhova (1979). Fatty acid methyl esters were obtained by using acid methanolysis as described by Rowe et al (2000).…”

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confidence: 99%

Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge

Romanenko¹,

Uchino²,

Tanaka³

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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An aerobic, Gram-negative bacterium, strain KMM 329 T , was isolated from a deep-sea sponge specimen from the Philippine Sea and subjected to a polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis showed that strain KMM 329 T clustered with the species of the genus Lysobacter. The highest level of 16S rRNA gene sequence similarity (97.0 %) was found with respect to Lysobacter concretionis KCTC 12205 T ; lower values (96.4-95.2 %) were obtained with respect to the other recognized Lysobacter species. The value for DNA-DNA relatedness between strain KMM 329 T and L. concretionis KCTC 12205 T was 47 %. Branched fatty acids 16 : 0 iso, 15 : 0 iso, 11 : 0 iso 3-OH and 17 : 1 iso were found to be predominant. Strain KMM 329 T had a DNA G+C content of 69.0 mol%. On the basis of the phenotypic, chemotaxonomic, DNA-DNA hybridization and phylogenetic data, strain KMM 329 T represents a novel species of the genus Lysobacter, for which the name Lysobacter spongiicola sp. nov. is proposed. The type strain is KMM 329 T (5NRIC 0728 T 5JCM 14760 T ).

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HPTLC of phospholipid mixtures containing phosphatidylglycerol

Cited by 89 publications

References 23 publications

Marinomonas pontica sp. nov., isolated from the Black Sea

Marinomonas pontica sp. nov., isolated from the Black Sea

Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge

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