HPTLC quantitation of 2-hydroxy-4-methoxybenzaldehyde in<i>Hemidesmus indicus</i>R.Br. root powder and extract

Darekar, Rahul S.; Khetre, Amol B.; Singh, Shradhanjali; Damle, Mrinalini C.

doi:10.1556/jpc.22.2009.6.13

Cited by 13 publications

(3 citation statements)

References 11 publications

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“…From the emerging aqueous phases the alkaloids were extracted by CH 2 Cl 2 after alkalinization with Na 2 CO 3 up to pH 9. Voacamine ( 1 ), [α] D 20 −54 ( c 1.2, CHCl 3 ) was identified by comparison of its spectroscopic data ( 1 H NMR, 13 C NMR, EIMS) with literature values. ,− The purity of voacamine ( 1 ) (95.2%) was determined by HPTLC densitometry. − The alkaloid was dissolved in DMSO (Panreacquimicasa, Barcelona, Spain) and then diluted, at the final concentration, in culture medium.…”

Section: Methodsmentioning

confidence: 99%

Voacamine Modulates the Sensitivity to Doxorubicin of Resistant Osteosarcoma and Melanoma Cells and Does Not Induce Toxicity in Normal Fibroblasts

Condello

Cosentino²,

Corinti³

et al. 2014

J. Nat. Prod.

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In previous studies it has been demonstrated that the plant alkaloid voacamine (1), used at noncytotoxic concentrations, enhanced the cytotoxicity of doxorubicin and exerted a chemosensitizing effect on cultured multidrug-resistant (MDR) U-2 OS-DX osteosarcoma cells. The in vitro investigations reported herein gave the following results: (i) the chemosensitizing effect of 1, in terms of drug accumulation and cell survival, was confirmed using SAOS-2-DX cells, another MDR osteosarcoma cell line; (ii) compound 1 enhanced the cytotoxic effect of doxorubicin also on the melanoma cell line Me30966, intrinsically drug resistant and P-glycoprotein-negative; (iii) at the concentrations used to sensitize tumor cells, 1 was not cytotoxic to normal cells (human fibroblasts). These findings suggest possible applications of voacamine (1) in integrative oncologic therapies against resistant tumors.

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Section: Methodsmentioning

confidence: 99%

Voacamine Modulates the Sensitivity to Doxorubicin of Resistant Osteosarcoma and Melanoma Cells and Does Not Induce Toxicity in Normal Fibroblasts

Condello

Cosentino²,

Corinti³

et al. 2014

J. Nat. Prod.

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“…Baicalein ( 1 ) and the other compounds were from Sigma-Aldrich (St. Louis, MO, USA). The purity of baicalein ( 1 ) (95.2%) was determined by HPTLC densitometry …”

Section: Methodsmentioning

confidence: 99%

“…The purity of baicalein (1) (95.2%) was determined by HPTLC densitometry. 49 Cell Culture. ZR-75-1 human breast cancer cells were purchased from Bioresource Collection and Research Center (Taiwan) and were cultured in RPMI-1640 medium supplemented with 10% heatinactivated fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in a humidified 5% CO 2 atmosphere.…”

Section: Journal Of Natural Productsmentioning

confidence: 99%

The Mechanism of Ca²⁺ Movement in the Involvement of Baicalein-Induced Cytotoxicity in ZR-75-1 Human Breast Cancer Cells

et al. 2015

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Baicalein (5,6,7-trihydroxyflavone) (1) has been found to be active against a wide variety of cancer cells. However, the molecular mechanism underlying the effects of 1 on the induction of Ca(2+) movement and cytotoxicity in human breast cancer cells is unknown. This study examined the relationship between 1-induced Ca(2+) signaling and cytotoxicity in ZR-75-1 human breast cancer cells. The in vitro investigations reported herein produced the following results: (i) Compound 1 increased intracellular Ca(2+) concentration ([Ca(2+)]i) in a concentration-dependent manner. The signal was decreased by approximately 50% by removal of extracellular Ca(2+). (ii) Compound 1-triggered [Ca(2+)]i increases were significantly suppressed by store-operated Ca(2+) channel blockers 2-aminoethoxydiphenyl borate (2-APB) and the PKC inhibitor GF109203X. (iii) In Ca(2+)-free medium, compound 1-induced [Ca(2+)]i increases were also inhibited by GF109203X. Furthermore, pretreatment with the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (TG) or 2,5-ditert-butylhydroquinone (BHQ) abolished 1-induced [Ca(2+)]i increases. Inhibition of phospholipase C (PLC) with U73122 abolished 1-induced [Ca(2+)]i increases. (iv) Compound 1 (20-40 μM) caused cytotoxicity, increased reactive oxygen species (ROS) production, and activated caspase-9/caspase-3. Furthermore, compound 1-induced apoptosis was significantly inhibited by prechelating cytosolic Ca(2+) with BAPTA-AM (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester) or by decreasing ROS with the antioxidant NAC (N-acetylcysteine). Together, baicalein (1) induced a [Ca(2+)]i increase by inducing PLC-dependent Ca(2+) release from the endoplasmic reticulum and Ca(2+) entry via PKC-dependent, 2-APB-sensitive store-operated Ca(2+) channels. Moreover, baicalein (1) induced Ca(2+)-associated apoptosis involved ROS production in ZR-75-1 cells.

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Current Awareness in Phytochemical Analysis

2010

Phytochemical Analysis

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley &Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of phytochemical analysis. Each bibliography is divided into 16 sections: 1 Reviews; 2 General; 3 Nucleic Acids; 4 Amino Acids, Proteins &Enzymes; 5 Carbohydrates; 6 Lipids; 7 Secondary Products ‐ General; Pharmacognosy; 8 Growth Regulators; 9 Industrially‐Important Products ‐ General; Food; Alcoholic beverages; 10 Toxins/Allergens; 11 Pigments; 12 Vitamins; 13 Others. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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HPTLC quantitation of 2-hydroxy-4-methoxybenzaldehyde inHemidesmus indicusR.Br. root powder and extract

Cited by 13 publications

References 11 publications

Voacamine Modulates the Sensitivity to Doxorubicin of Resistant Osteosarcoma and Melanoma Cells and Does Not Induce Toxicity in Normal Fibroblasts

Voacamine Modulates the Sensitivity to Doxorubicin of Resistant Osteosarcoma and Melanoma Cells and Does Not Induce Toxicity in Normal Fibroblasts

The Mechanism of Ca²⁺ Movement in the Involvement of Baicalein-Induced Cytotoxicity in ZR-75-1 Human Breast Cancer Cells

Current Awareness in Phytochemical Analysis

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HPTLC quantitation of 2-hydroxy-4-methoxybenzaldehyde inHemidesmus indicusR.Br. root powder and extract

Cited by 13 publications

References 11 publications

Voacamine Modulates the Sensitivity to Doxorubicin of Resistant Osteosarcoma and Melanoma Cells and Does Not Induce Toxicity in Normal Fibroblasts

Voacamine Modulates the Sensitivity to Doxorubicin of Resistant Osteosarcoma and Melanoma Cells and Does Not Induce Toxicity in Normal Fibroblasts

The Mechanism of Ca2+ Movement in the Involvement of Baicalein-Induced Cytotoxicity in ZR-75-1 Human Breast Cancer Cells

Current Awareness in Phytochemical Analysis

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The Mechanism of Ca²⁺ Movement in the Involvement of Baicalein-Induced Cytotoxicity in ZR-75-1 Human Breast Cancer Cells