HPV E6/E7: insights into their regulatory role and mechanism in signaling pathways in HPV-associated tumor

Peng, Qiu; Wang, Lujuan; Zuo, Liang; Gao, Shuichao; Jiang, Xianjie; Han, Yaqian; Lin, Jinguan; Peng, Mingjing; Wu, Nayiyuan; Tang, Yanyan; Tian, Hao; Zhou, Yujuan; Liao, Qianjin

doi:10.1038/s41417-023-00682-3

Cited by 19 publications

(5 citation statements)

References 130 publications

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“…However, it is also found in cervical carcinoma sometimes [17]. HPV-encoded E6 and E7 proteins that target the negative cell cycle regulators such as p105Rb and p53 are the most important viral oncoproteins during cancer development [18]. Persistent infection with high-risk HPV types is one of the risk factors of cervical cancer [19].…”

Section: Discussionmentioning

confidence: 99%

“…HPV DNA detection and genotyping was performed on an ABI7500 real-time polymerase chain reaction genotyping platform using two commercial HPV DNA PCR assays, namely GeneProof Human Papillomavirus (HPV) Screen PCR Kit (GeneProof, Brnojih, Czech Republic) and Yaneng Human Papillomavirus Nucleic Acid Detection and HPV16/18 Genotyping Kit (PCR-Fluorescence Probing) (Yaneng BIOscience, Shenzhen, China), according to the manufacturer's instructions. The GeneProof kit can detect and identify high-risk HPV types 16,18,26,30,31,33,34,35,39,45,51,52,53,56,58,59, 66, 67, 68, 69, 70, 73, 82, 97, whereas the Yaneng kit can detect and identify high-risk HPV types 16,18,26,31,33,35,39,45,51,52,53,56,58,59, 66, 68, 73, 82.…”

Section: Menstrual Blood Hpv Pcrmentioning

confidence: 99%

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Menstrual Blood as a Diagnostic Specimen for Human Papillomavirus Genotyping and Genital Tract Infection Using Next-Generation Sequencing as a Novel Diagnostic Tool

Tsang,

Cheung,

et al. 2024

Diagnostics

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Background: Menstrual blood (MB) is a convenient specimen type that can be self-collected easily and non-invasively by women. This study assessed the potential application of MB as a diagnostic specimen to detect genital tract infections (GTIs) and human papillomavirus (HPV) infections in women. Method: Genomic DNA was extracted from MB samples. Pacific Bioscience (Pacbio) 16S ribosomal DNA (rDNA) high-fidelity (HiFi) long-read sequencing and HPV PCR were performed. Results: MB samples were collected from women with a pathological diagnosis of CIN1, CIN2, CIN3 or HPV infection. The sensitivity and positive predictive value (PPV) of high-risk HPV detection using MB were found to be 66.7%. A shift in vaginal flora and a significant depletion in Lactobacillus spp. in the vaginal microbiota communities were observed in the MB samples using 16S rDNA sequencing. Conclusions: In this study, we demonstrated that MB is a proper diagnostic specimen of consideration for non-invasive detection of HPV DNA and genotyping using PCR and the diagnosis of GTIs using metagenomic next-generation sequencing (mNGS). MB testing is suitable for all women who menstruate and this study has opened up the possibility of the use of MB as a diagnostic specimen to maintain women’s health.

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Section: Discussionmentioning

confidence: 99%

Section: Menstrual Blood Hpv Pcrmentioning

confidence: 99%

Menstrual Blood as a Diagnostic Specimen for Human Papillomavirus Genotyping and Genital Tract Infection Using Next-Generation Sequencing as a Novel Diagnostic Tool

Tsang,

Cheung,

et al. 2024

Diagnostics

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“…Human papillomavirus (HPV) is a non-enveloped circular double-stranded DNA virus [ 1 ]. The genome can be divided into early protein coding region (E region), late protein coding region (L region) and long control region (LCR).…”

Section: Introductionmentioning

confidence: 99%

Interferon-γ-induced GBP1 is an inhibitor of human papillomavirus 18

Xu,

Lin,

2024

BMC Women's Health

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Background Human papillomavirus (HPV) infection is an important factor leading to cervical cell abnormalities. 90% of cervical cancers are closely associated with persistent infection of high-risk HPV, with the highest correlation with HPV16 and 18. Currently available vaccines and antivirals have limited effectiveness and coverage. Guanylate binding protein 1 (GBP1) was induced by interferon gamma and involved in many important cellular processes such as clearance of various microbial pathogens. However, whether GBP1 can inhibit human papillomavirus infection is unclear. Results In this study, we found that GBP1 can effectively degrade HPV18 E6, possibly through its GTPase activity or other pathways, and E6 protein degrades GBP1 through the ubiquitin-proteasome pathway to achieve immune escape. Conclusion Therefore, GBP1 is an effector of IFN-γ anti-HPV activity. Our findings provided new insights into the treatment of HPV 18 infections.

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“…Persistent HPV infection is identified as the primary cause in the development of cervical intraepithelial lesions, occurring in the majority of CC patients ( Shamseddine et al, 2021 ). The viral oncoproteins E6 and E7 perform central roles on HPV-induced carcinogenic processes by affecting cell cycle and proliferation ( Peng et al, 2024 ). Recently, integrating single-cell sequencing and spatial transcriptome revealed the mechanism of HPV-related CC carcinogenesis in terms of both the malignant transition of epithelial cells and the remodeling of the immune microenvironment ( Guo et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Cervical HPV infection and related diseases among 149,559 women in Fujian: an epidemiological study from 2018 to 2023

Lin,

Hong,

et al. 2024

Front. Microbiol.

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ObjectivesTo comprehensively analyze the epidemiological features of human papillomavirus (HPV) and HPV-related cervical diseases in females aged 35–64 years.MethodsA total of 149,559 samples of exfoliated cervical cells screened for HPV and related cervical lesions from January 2018 to December 2023 were enrolled. The prevalence of 15 high-risk and 6 low-risk HPV genotypes were detected, and the cervical cytology were analyzed. The impact of single and multiple HPV infections was characterized, and the effect of age was studied.ResultsThe cervix cytology was normal in 86.60% of the females, while 7.13% of the females were diagnosed with cervix inflammation, 0.60% with ASC-US, 0.22% with ASC-H, 0.72% with LSIL, 0.49% with HSIL, 0.03% with ICC. The highest median age was observed in ASC-H group with 54 years old. Females with primary school education or lower have the highest positive rates. The overall HPV prevalence was 8.60%. The relatively prevalent HPV types were HPV52, 58, 16, 39, 51. HPV16, HPV18, HPV58, HPV33 and HPV52 were the top5 predominant types in ICC patients. 17.41% females suffered from multiple HPV infection with the most frequently co-infection subtypes being HPV52, HPV58 and HPV16. The prevalence of all HPV subtypes increased with age. Multiple HPV infections accounted for a larger proportion in those aged above 55 years. The peak HPV16 prevalence was observed in ICC group in cases aged 45–49 and 55–59. The peak HPV33 prevalence was observed in younger individuals aged 40–44 who developed ICC.ConclusionMore action should be taken against HPV33 infection.

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HPV E6/E7: insights into their regulatory role and mechanism in signaling pathways in HPV-associated tumor

Cited by 19 publications

References 130 publications

Menstrual Blood as a Diagnostic Specimen for Human Papillomavirus Genotyping and Genital Tract Infection Using Next-Generation Sequencing as a Novel Diagnostic Tool

Menstrual Blood as a Diagnostic Specimen for Human Papillomavirus Genotyping and Genital Tract Infection Using Next-Generation Sequencing as a Novel Diagnostic Tool

Interferon-γ-induced GBP1 is an inhibitor of human papillomavirus 18

Cervical HPV infection and related diseases among 149,559 women in Fujian: an epidemiological study from 2018 to 2023

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