HPV genotype distribution according to severity of cervical neoplasia

Sjoeborg, Katrine D.; Tropé, Ameli; Lie, A. Kathrine; Jonassen, Christine Monceyron; Steinbakk, Martin; Hansen, Mona; Jacobsen, Morten; Cuschieri, Kate; Eskild, Anne

doi:10.1016/j.ygyno.2010.03.007

Cited by 51 publications

(45 citation statements)

References 30 publications

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“…In this study, HPV was detected in 81.0% of the examined population. This high rate of HPV prevalence correlate with some previously published studies [26][27][28][29]. [33] and Milutin-Gasperov [34].…”

Section: Discussionsupporting

confidence: 90%

Tissue Detection and Typisation of Human Papillomavirus in Women with Squamous Intraepithelial Lesions and Squamous Invasive Carcinoma of the Cervix

Dabeski¹,

Danilovski²,

Antovska³

et al. 2015

Macedonian Medical Review

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Introduction. The most common risk factor for intraepithelial lesions and cervical carcinoma is infection with human papillomavirus (HPV), especially with high-risk HPV genotypes. Only persistent, high-risk HPV infections represent a major risk factor for intraepithelial lesions and cervical cancer. The aims of the study were: detection and typisation of HPV genotypes, which are the most common causes of intraepithelial lesions and cervical cancer, determination of the correlation between HPV infection and histopathological diagnosis, and the correlation between the grade of lesion of the cervix and oncogenic potential of the virus as well as determination of the most affected age group of patients. Methods. This cross-sectional study included 100 sexually active patients with an abnormal Pap test at the age from 20 to 69 years (39±10.77), and was conducted at the University Clinic of Gynecology and Obstetrics in Skopje and University Clinic of Radiotherapy and Oncology in Skopje in the period from January 2014 to August 2014. In all patients colposcopic cervical biopsy was made with endocervical curettage for histopathological analysis and cervical biopsy for detection and HPV typisation. HPV detection and typisation were done using polymerase chain reaction (PCR) and reverse hybridization. Results. HPV DNA was detected in 81.0% (81/100) of the examined women. The relationship between the prevalence of high-risk and low-risk HPV DNA genotypes was 72.0%:9.0%. The frequency of high-risk HPV DNA genotypes ranged from: 54.5% (12/22) in productive HPV infection-mild dysplasia, 86.4% (19/22) with moderate dysplasia, 91.2% (21/23) in severe dysplasia

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Section: Discussionsupporting

confidence: 90%

Tissue Detection and Typisation of Human Papillomavirus in Women with Squamous Intraepithelial Lesions and Squamous Invasive Carcinoma of the Cervix

Dabeski¹,

Danilovski²,

Antovska³

et al. 2015

Macedonian Medical Review

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“…This proportion is equivalent to that found in abnormal Pap tests from a populationbased screening program [21], in HIV-positive Brazilian women [22] and in a study of worldwide HPV genotype distribution in cervical cancer [12]. While HPV infection was detected in 98.4% of women with histologically confirmed CIN2 or higher [23]. The presence of cervical lesions in the populations studied could be the reason for the similarity in the prevalence of HPV infection.…”

Section: Discussionsupporting

confidence: 50%

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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BackgroundCervical cancer is the second most common cancer in women worldwide and the most common cause of mortality in underdeveloped and developing countries [1,2]. Persistent infection with certain oncogenic high-risk (HR) types of Human Papillomavirus (HPV) is the required factor for the development of invasive cervical cancer and precursor lesions [3][4][5][6].Overall, the prevalence and distribution of HPV infection in cervical lesions has been determined in many geographical regions of the world, this can vary depending on several factors including epidemiological differences in the populations studied and the methodology used for molecular detection and typing of HPV DNA [7]. Over 120 types of HPV have been classified as either low (LR-HPV) or high risk (HR-HPV), according to their oncogenic potential [8,9]. Within the high-risk genotypes, HPV types 16 and HPV 18 are most often associated with cancer and squamous intraepithelial lesion [5,6,10,11,12]. Nevertheless, the risk of neoplasia for other types of HPV as well as for multiple HPV infection has not yet been established.Because current strategies for the prevention of cervical cancer and their precancerous lesions are based on the HPV genotyping [13] and prophylactic vaccines [14][15][16], it seems necessary to determine the types of HPV most commonly associated with malignant cell transformation in different geographical areas in order to establish effective preventive measures according to the epidemiology of the population. This study was to know the prevalence and distribution of cervical HPV infection in women attending routine cervical cancer screening in the Valencia region (Spain). Study designLiquid-based cytology (LBC) of cervical specimens was collected during routine screening visits, between June 20, 2011 and September 9, 2011 in the Clinical Hospital of Valencia, Spain. LBC samples were processed using the Thin Prep® 5000 processor (Madrid, Spain). For HPV detection and genotyping, we used the residual cervical sample material available from the same Thin Prep Pap test vial previously used for cytological screening. DNA was extracted from the residual materials of LBC by QIAamp® DNA mini kit (IZASA, Valencia, Spain), according to the manufacturer's instructions. HPV testing was performed by INNO-Lipa HPV genotyping Extra Reverse Hybridization Line Probe Assay kit (Innogenetics®, Barcelona, Spain), according to the manufacturer's instructions. Briefly, HPV detection and genotyping was based on PCR amplification of a 65pb fragment, within the L1 region of the HPV genome, using broad-spectrum SPF10 biotinylated primers. PCR was performed in a final AbstractBackground: Infection with human papillomavirus (HPV) has been identified as the primary cause of cervical cancer. Persistent infection with high risk-HPV is the required factor for the development of cervical cancer.

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“…Types 16 and 18 are associated with a higher probability of progression to malignancy, even in patients with small cytological alterations (ASC-US and LSIL), who exhibit E6/E7 mRNA expression (Clifford et al, 2005;Brismar-Wendel et al, 2009;Lizano et al, 2009;Muñoz et al, 2009;Sjoeborg et al, 2010;Correnti et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Expression of human papillomavirus E6 and E7 oncoprotein mRNA in women with low-grade squamous intraepithelial lesions or less

et al. 2016

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ABSTRACT. We verified the prevalence of human papillomavirus (HPV) E6/E7 protein mRNA expression in patients with low-grade squamous intraepithelial lesions (LSILs) and negative cervicovaginal cytology. To investigate the relationship between mRNA expression and viral infection type, we assessed genotyping in single infections. Samples from 825 women were submitted to the E6/E7 survey. We noticed a larger percentage of E6/E7 mRNA expression in the atypical squamous cells of undetermined significance (ASC-US) and LSIL cytologies. Negative results of mRNA expression were in accordance with negative cytologies. In positive cases, the infection by a single HPV type was most common, with type 16 being most prevalent. The expression of mRNA was most prevalent in ASC-US and LSIL cytologies, compared with the negative cytology. The infection by a HPV type was more frequent in cases of positive expression, with HPV

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HPV genotype distribution according to severity of cervical neoplasia

Cited by 51 publications

References 30 publications

Tissue Detection and Typisation of Human Papillomavirus in Women with Squamous Intraepithelial Lesions and Squamous Invasive Carcinoma of the Cervix

Tissue Detection and Typisation of Human Papillomavirus in Women with Squamous Intraepithelial Lesions and Squamous Invasive Carcinoma of the Cervix

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Expression of human papillomavirus E6 and E7 oncoprotein mRNA in women with low-grade squamous intraepithelial lesions or less

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