HPV Prevalence and Detection of Rare HPV Genotypes in Hong Kong Women from Southern China with Cytological Abnormalities

Abstract

Human papillomavirus (HPV) has been identified as the primary cause of cervical squamous intraepithelial lesion and invasive cervical cancer. The emergence of various commercial HPV genotyping kits with different characteristics facilitates the detection of most high-risk and low-risk HPV genotypes, but the rare HPV types are usually underdiagnosed. In the present study, HPV detection was performed using the GenoFlow HPV Array Test kit (DiagCor Bioscience), which can identify 33 HPV subtypes by specific probes. Besides, a HPV consensus probe (universal probe) was designed to capture not only the 33 genotypes but also rare subtypes. Of the 1643 Southern Chinese women tested between 2012 and 2013, the HPV prevalence was 42.3%, with HPV 52 (139/1643, 8.5%), HPV 81 (89/1643, 5.4%), and HPV 16 (63/1643, 3.8%) being the most frequent subtypes detected. Among all 695 HPV-positive cases, 56 (8.1%) cases were only detected by the universal probe, in which 5 were either ASCUS or LSIL cases. Sequencing results confirmed HPV types 30, 91, and 74, and the intratypic variants of HPV 72 and 82 were present in the 5 cases. The result suggests that some rare HPV subtypes might be involved in cervical lesions.

Cervical Dysplasia, Infection, and Phylogeny of Human Papillomavirus in HIV‐Infected and HIV‐Uninfected Women at a Reproductive Health Clinic in Nairobi, Kenya

“…In this study, HPV 81 was the only genotype detected in a woman with HSIL. High prevalence of HPV 81 was also detected in women with abnormal cytology elsewhere [39,40]. HPV 81 is recognized as a new type mostly found in patients with a weak immune system [40].…”

“…High prevalence of HPV 81 was also detected in women with abnormal cytology elsewhere [39,40]. HPV 81 is recognized as a new type mostly found in patients with a weak immune system [40]. This type is associated with precancerous and cancerous lesions [39].…”

Nested PCR followed by NGS: Validation and application for HPV genotyping of Tunisian cervical samples

“…All the clinical samples were typed during a previous study using RLH as previously described [13]. Briefly, the technique permits the identification of 31 types using specific probes (HPV6, 11,16,18,26,31,33,34,35,39,40,42,44,45,51,52,53,54,55,56,57,58,59, 66, 68, 69, 70, 73, 82, 83, and 84) that were immobilized on a Biodyne C membrane.…”

“…NGS identified novel LR-HPV (HPV81, HPV43, HPV62 and HPV74) that are considered as rare HPV types [33] and limited studies reported their prevalence. These types were not described so far in Tunisian studies neither in healthy women nor in women with precancerous and cancerous lesions [13,[34][35][36], which further indicates its relevance for epidemiology.…”

Prevalence of human papilloma virus (HPV) genotypes between outpatients males and females referred to seven laboratories in Tehran, Iran

“…According to Institut Català d’Oncologia and International Agency for Research on Cancer Information Centre on HPV and Cancer (ICO/IARC) report released on 2018, about 917 new cervical cancer cases are diagnosed annually in Iran. Also, cervical cancer ranked as the 16th leading cause of female cancer which accounted as being the 10th most common female cancer in women aged 15–44 years old in Iran [ 10 ]. In line with the Iranian published data, the prevalence of HPV in different female cervical specimens has been reported to be: between 5.5 and 9.4% in normal cytology specimens [ 11 – 13 ]; between 61.7 and 65.3% in Cervical Intraepithelial Neoplasia (I–III) samples [ 12 , 14 , 15 ] and between 75.2 and 87% in cervical cancer specimens with a high heterogeneity among studies [ 16 – 18 ].…”