HPV Testing from Dried Urine Spots as a Tool for Cervical Cancer Screening in Low-Income Countries

Frati, Elena Rosanna; Martinelli, Marianna; Fasoli, Ester; Colzani, Daniela; Bianchi, Silvia; Binda, Sandro; Olivani, Pierfranco; Tanzi, Elisabetta

doi:10.1155/2015/283036

Cited by 14 publications

(12 citation statements)

References 20 publications

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“…Previously published studies have in fact reported a high prevalence of the HPV 51 genotype in Italian women [15,18,19]. The high prevalence of HPV 16 is in keeping with the worldwide spread of this genotype, not only among women but also in the male population [6,9,14,20,21].…”

Section: Discussionmentioning

confidence: 75%

Prevalence of Human Papillomavirus (HPV) and Other Sexually Transmitted Infections (STIs) among Italian Women Referred for a Colposcopy

Martinelli

Musumeci

Sechi

et al. 2019

IJERPH

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Sexually transmitted infections (STIs) represent a major cause of morbidity in women and men worldwide. Human Papillomavirus (HPV) infections are among the most prevalent STIs and persistent infections with high-risk HPV (hrHPV) genotypes can cause cervical dysplasia and invasive cervical cancer. The association of other STIs with HPV cervical infection and/or dysplasia has however not yet been fully elucidated. The aim of this study was to assess the prevalence of HPV and other STIs among women presenting with an abnormal cervical cytology. Cervical infections with 28 HPV genotypes and seven other sexually transmitted pathogens were evaluated in 177 women referred for a colposcopy after an abnormal Pap smear. Positivity for at least one hrHPV genotype was shown in 87% of women; HPV 16 was the most prevalent (25.0%), followed by HPV 31 and HPV 51. The overall positivity for other STIs was 49.2%, with Ureaplasma parvum being the most prevalent microrganism (39.0%). Co-infections between hrHPV and other STIs were demonstrated in 17.5% of women; no significant association was demonstrated between multiple infections and the colposcopy findings. This study provides new epidemiological data on the prevalence of cervical infections associated with HPV and seven other common sexually transmitted pathogens in a population of women presenting with an abnormal cervical cytology.

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Section: Discussionmentioning

confidence: 75%

Prevalence of Human Papillomavirus (HPV) and Other Sexually Transmitted Infections (STIs) among Italian Women Referred for a Colposcopy

Martinelli

Musumeci

Sechi

et al. 2019

IJERPH

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“…Epidemiological and biological studies found that HPV16 is the most frequently detected HPV genotype in patients with cervical cancer worldwide [6]. Moreover, it is also the predominant infection identified among subjects with or without genital lesions in different groups of the population [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Human Papillomavirus (HPV) 16 Variants Associated with Cervical Infection in Italian Women

Martinelli

Clara

Sotgiu

et al. 2020

IJERPH

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This study aims to evaluate HPV16 variants distribution in a population of Italian women living in two different regions (Lombardy and Sardinia) by sequence analyses of HPV16-positive cervical samples, in order to reconstruct the phylogenetic relationship among variants to identify the currently circulating lineages. Analyses were conducted starting from DNA isolated from 67 HPV16-positive cervical samples collected from two different Italian centres (31 from Lombardy and 36 from Sardinia) of women with normal and abnormal cervical cytology. The entire long control region (LCR) and 300 nt of the E6 gene was sequenced to identify intra-type variants. Sequence comparison and phylogenetic analysis were made using a distance-based neighbour joining method (NJ) and Kimura two-parameter model. Data obtained reported that Italian sequences mainly belonged to the European lineage, in particular sublineage A2. Only five sequences clustered in non-European branches: two in North American lineage (sublineage D1), two in African-1 (sublineage B1) and one in African-2. A new 27 nucleotide duplication in the central segment of the LCR region was found in a sequence obtained from a sample isolated in Sardinia. A predominance of European variants was detected, with some degree of variability among the studied HPV16 strains. This study contributes to the implementation of data regarding the molecular epidemiology of HPV16 variants.

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“…Another much less utilized, but very similar sampling approach is the analysis of dried urine on filter paper, called dried urine strips (DUS). This approach has previously been used in other applications such as creatinine testing [ 25 , 26 ], HPV (human papillomavirus) testing [ 27 ], and metabolomics analysis [ 28 , 29 ]. Herein, we report on a DBS/DUS-HILIC-LC-MS/MS protocol development for analysis of the human blood and urine metabolome ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips

Drolet¹,

Tolstikov²,

Williams³

et al. 2017

Metabolites

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(1) Background: Interest in the application of metabolomics toward clinical diagnostics development and population health monitoring has grown significantly in recent years. In spite of several advances in analytical and computational tools, obtaining a sufficient number of samples from patients remains an obstacle. The dried blood spot (DBS) and dried urine strip (DUS) methodologies are a minimally invasive sample collection method allowing for the relative simplicity of sample collection and minimal cost. (2) Methods: In the current report, we compared results of targeted metabolomics analyses of four types of human blood sample collection methods (with and without DBS) and two types of urine sample collection (DUS and urine) across several parameters including the metabolite coverage of each matrix and the sample stability for DBS/DUS using commercially available Whatman 903TM paper. The DBS/DUS metabolomics protocols were further applied to examine the temporal metabolite level fluctuations within hours and days of sample collection. (3) Results: Several hundred polar metabolites were monitored using DBS/DUS. Temporal analysis of the polar metabolites at various times of the day and across days identified several species that fluctuate as a function of day and time. In addition, a subset of metabolites were identified to be significantly altered across hours within a day and within successive days of the week. (4) Conclusion: A comprehensive DBS/DUS metabolomics protocol was developed for human blood and urine analyses. The described methodology demonstrates the potential for enabling patients to contribute to the expanding bioanalytical demands of precision medicine and population health studies.

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HPV Testing from Dried Urine Spots as a Tool for Cervical Cancer Screening in Low-Income Countries

Cited by 14 publications

References 20 publications

Prevalence of Human Papillomavirus (HPV) and Other Sexually Transmitted Infections (STIs) among Italian Women Referred for a Colposcopy

Prevalence of Human Papillomavirus (HPV) and Other Sexually Transmitted Infections (STIs) among Italian Women Referred for a Colposcopy

Analysis of Human Papillomavirus (HPV) 16 Variants Associated with Cervical Infection in Italian Women

Integrated Metabolomics Assessment of Human Dried Blood Spots and Urine Strips

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