HR-MAS NMR Allied to Chemometric on Hancornia speciosa Varieties Differentiation

Abstract

This work describes the potential of chemometric analyses applied to 1 H high-resolution magic angle spinning nuclear magnetic resonance ( 1 H HR-MAS NMR) data for the chemotaxonomic investigation of Hancornia speciosa (Apocynaceae) varieties. This plant, popularly known as mangaba, has a complex morphological differentiation and thus chemical analyses can be used for their taxonomic classification. In comparison to traditional techniques, 1 H HR-MAS NMR allied with chemometrics provided a simple and low cost method for chemotaxonomy. Leaves of four varieties of H. speciosa from a common garden experiment was studied and demonstrated that H. speciosa var. speciosa differs from others due to its specific metabolic profile, and var. pubescens was discriminated based on its high phenolic compound content. The distinction between the latter variety and gardineri is important once it allows for the selection of samples with greater commercial value, once they produce the largest and heaviest fruits.

Genetic structure of Hancornia speciosa Gomes (Apocynaceae) in the cerraro and atlantic forest domains reveal two botanical varieties

“…pubescens generally clustered together. Other studies using chemometric analyses showed that H. speciosa var speciosa differs from other varieties (Flores et al 2017). Our phylogenetic analysis shows correlation with morphological descriptions suggested by Monachino (1945), in which the varieties H. speciosa var.…”

“…(Monachino, 1945). The identi cation of these botanical varieties is based in the morphological characters; however, the origin of the genetic relationships and the putative hybrids of the botanical varieties is not clear (Collevatii et al 2016;Flores et al 2017). The botanical varieties are dispersed in the Atlantic Rain forest, Cerrado and Caatinga domains, of which the differences among biomes is suitable for phylogeographic and phylogenetic studies, which may be useful to understand of the genetic diversi cation of H speciosa.…”

Agronomic evaluation of a Hancornia speciosa Gomes germplasm collection from the Brazilian Cerrado

“…Controlled pollination experiment demonstrated that the species is self-incompatible (Darrault and Schlidwein 2005) that was corroborated by pollen dispersal study (Collevatti et al 2016). Some works have shown the existence of genetic variability among and/or within botanical varieties based on quantitative traits (Ganga et al 2009), allozymes (Martins et al 2012), DNA molecular markers (Moura et al 2005, Silva et al 2011, Rodrigues et al 2015, Collevatti et al 2016, Costa et al 2017) and chemical components (Flores et al 2018).…”

Important issues in plant tissues analyses by HR‐MAS NMR

“…Moreover, it has been especially used in the analysis of foodstuffs, such as the identification of lipids, carbohydrates, and small metabolites . This technique has several advantages in metabolomic studies, as it permits plant genotypes to be differentiated such as genetically modified organisms, different phenotypes, and chemotaxonomy identification . HR‐MAS NMR is also widely used for quality control, such as determining the purity, authenticity, and origin of several plant‐based products …”

“…3,[7][8][9][10] This technique has several advantages in metabolomic studies, as it permits plant genotypes to be differentiated such as genetically modified organisms, 11 different phenotypes, 12,13 and chemotaxonomy identification. 4,14,15 HR-MAS NMR is also widely used for quality control, such as determining the purity, authenticity, and origin of several plant-based products. 16 Additionally, identifying compounds in complex samples is important for the raw-material authentication and origin certification used as phytotherapeutics.…”