HriGFP Novel Flourescent Protein: Expression and Applications

Saeed, Salma; Mehreen, Hira; Gerlevik, Umut; Tariq, Aamira; Manzoor, Saira; Noreen, Zobia; Sezerman, Uğur; Bokhari, Habib

doi:10.1007/s12033-020-00243-1

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…Moreover, the highly sensitive fluorescent properties of fluorescent proteins have been utilized in vitro to monitor the expression level of membrane proteins [7,8]. A number of fluorescence characteristics of fluorescent proteins have already been reported in various application fields and their potential is far from being fully discovered [2,4,[9][10][11]. One interesting optical property of fluorescent proteins is that their fluorescence intensity changes depending on the external factors, such as pH or the presence of metal ions [12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and Structural Analysis of Cu2+-Induced Fluorescence Quenching of ZsYellow

Kim

Nam

2020

Biosensors

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Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each fluorescent protein exhibits unique spectroscopic properties and mechanisms for fluorescence quenching by metals. Therefore, the metal-induced fluorescence quenching analysis of various new fluorescent proteins would be important step towards the development of such fluorescent protein-based metal biosensors. Here, we first report the spectroscopic and structural analysis of the yellow fluorescent protein ZsYellow, following its metal-induced quenching. Spectroscopic analysis showed that ZsYellow exhibited a high degree of fluorescence quenching by Cu2+. During Cu2+-induced ZsYellow quenching, fluorescence emission was recovered by adding EDTA. The crystal structure of ZsYellow soaked in Cu2+ solution was determined at a 2.6 Å resolution. The electron density map did not indicate the presence of Cu2+ around the chromophore or the β-barrel surface, which resulted in fluorescence quenching without Cu2+ binding to specific site in ZsYellow. Based on these results, we propose the fluorescence quenching to occur in a distance-dependent manner between the metal and the fluorescent protein, when these components get to a closer vicinity at higher metal concentrations. Our results provide useful insights for future development of fluorescent protein-based metal biosensors.

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