hsa‑miR‑15a‑5p inhibits colon cell carcinoma via targeting CCND1

Abstract

Colon carcinoma is one of the most common cancers worldwide. Epidemiological studies have revealed that colon cancer is the third leading cause of cancer-related deaths, which is due to the increased incidence and mortality rates. However, the treatment strategies for colon cancer remain unsatisfactory for patients, especially for those with advanced or recurrent colon cancer. Dysregulated microRNAs (miRNAs) are considered to influence tumor development and metastasis. However, the molecular mechanism through which miRNAs affect cancer progression is not yet completely understood. The aim of the present study was to investigate the expression levels of has-miR-15a-5p and its molecular mechanism in colon cell carcinoma. In the present study, the expression levels of hsa-miR-15a-5p were found to be decreased in colon tumor tissues and cancer cell lines. Hsa-miR-15a-5p overexpression inhibited colon cell proliferation and migration. Mechanistically, the G1/S-specific cyclin-D1 (CCND1) gene was predicted as a target of hsa-miR-15a-5p, as evidenced by bioinformatics and dual-luciferase reporter assay analyses. CCND1 overexpression significantly increased the progression of colon cancer. Furthermore, CCND1 was demonstrated to mediate the effects of hsa-miR-15a-5p on colon cancer cells. The present study demonstrated that hsa-miR-15a-5p alleviated the proliferation, migration and invasion of colon cancer by targeting the CCND1 gene, which represents a potential molecular target for the diagnosis and treatment of colon cancer.

Decreased SPTBN2 expression regulated by the ceRNA network is associated with poor prognosis and immune infiltration in low‑grade glioma

“…Higher expression of hsa-miR-15b-5p has been reported to be associated with a short survival time of patients with LGG (36). Has-miR-15a-5p promotes the proliferation and invasion of colorectal cancer by targeting CCND1 (37). Hsa-mir-16-5p is downregulated in giant cell tumors (38).…”

Systemic circulating microRNA landscape in Lynch syndrome

“…However, we could see that overexpression of hsa‐miR‐122b might result in reduced cell viability, plausibly due to increased apoptosis. Previous studies have linked hsa‐miR‐15a‐5p to sporadic endometrial cancer 46 and colorectal cancer, 47 both being hallmark cancers of LS. In our analysis, hsa‐miR‐15a‐5p was seen to target several genes, including known oncogenes and tumor suppressor genes such as CCND1 , CDK6 and DICER1 , thus suggesting biomarker potential also in LS.…”

Immunogenic cell death mediated TLR3/4-activated MSCs in U87 GBM cell line

“…It is supposed that hsa-miR-15a-5p is involved in crucial biological processes through its targeted gene, G1/S-specific cyclin-D1 (CCND1). Bioinformatics and dual-luciferase reporter assay analyses predicted CCND1 as a target of hsa-miR-15a-5p [ 64 ]. Additionally, a recent study revealed that miR-15a-5p promotes apoptosis and inhibits cell growth through the changes in expression levels of pro-survival genes and proteins, including Bcl-xl and Bcl-2 and inhibition of oncogenic transcription factors such as CCND2, Bcl-6, MYC, and PIK3R1 [ 65 ].…”