Hsa_circ_0000081 promotes the function of gastric cancer through sponging hsa-miR-423-5p to influence 3-phosphoinositide-dependent kinase 1 expression

Abstract

Gastric cancer (GC) is one of the most common malignancies in the world, and effective therapeutic targets need to be identified for this type of cancer. In this study, circular RNA (circRNA) microarray analysis was utilized to screen differentially expressed circRNA in GC. Using quantitative reverse transcription polymerase chain reaction (qRT-PCR), hsa_circ_0000081 (circRNA-0000081) expression was found to be up-regulated in tissues and cells and was negative correlated with patients’ survival time. RNase R and Actinomycin D assays indicated that circRNA-0000081 was significantly more resistant to R enzyme and had a longer half-life than linear RNA. Moreover, the knockdown or overexpression of circRNA-000081 could influence the proliferation, migration, and invasion potential of GC. Finally, dual luciferase reporter, RNA immunoprecipitation, qRT-PCR, and western blotting assays were used to verify the targeting relationship between circRNA-000081 and miRNA-423-5p or miRNA-423-5p and 3-phosphoinositide-dependent kinase 1 (PDPK1). In conclusion, circRNA-0000081 promotes the function of GC through sponging hsa-miR-423-5p to influence PDPK1 expression, which has a promising therapeutic potential for treating patients with GC.

Hsa_circ_0044301 Regulates Gastric Cancer Cell’s Proliferation, Migration, and Invasion by Modulating the Hsa-miR-188-5p/DAXX Axis and MAPK Pathway

“…CircRNA microarray was performed to investigate potential key circRNA in GC (Kang Chen Biotech, Shanghai, China) as we covered in our previous article [ 20 ].…”

miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis

“…miR-423 is also sponged by other lncRNAs in other cell types, such as LINC00680 in oesophageal squamous cell carcinoma cells [ 46 ] and AFAP1-AS1 in nasopharyngeal carcinoma cells [ 47 ]. A recent report showed that circRNA-0000081 maintains the levels of PDPK1 (a target of miR-423) in gastric cancer cells via miR-423 sponging, thereby affecting cell proliferation, migration, and invasion potential [ 48 ]. In the present study, we discovered a new signaling pathway, the NORHA /miR-423/ SMAD7 pathway, that contributes to FA and GC apoptosis and enriches the ceRNA network of the NORHA -miRNAs in GCs.…”

Lumican promotes gastric cancer progression by regulating the ERK pathway

“…All the aforementioned cell lines were meticulously nurtured in RPMI 1640 medium (Thermo Fisher Scientific Inc, USA), which was supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific Inc, USA) and 1% penicillin-streptomycin (comprising 10,000 U/mL penicillin + 10,000 µg/mL streptomycin, Thermo Fisher Scientific Inc, USA), which we used in the refer to this medium here as complete medium. The cultivation of these cells transpired under precisely controlled conditions, specifically at a temperature of 37°C , within an environment enriched with 5% CO2, within a dedicated carbon dioxide cell incubator [14].…”