Hsa_circ_0004370 promotes esophageal cancer progression through miR-1294/LASP1 pathway

Abstract

Circular RNAs (circRNAs) formed by back-splicing play multiple roles in the occurrence and development of cancer. Here, we found that hsa_circ_0004370 was up-regulated in both esophageal cancer (EC) tissues and cell lines. Loss function of hsa_circ_0004370 by si-RNA significantly suppressed proliferation and invasion and promoted apoptosis in both EC cell lines. The sponging of miR-1294 by hsa_circ_0004370 was bioinformatically predicted and subsequently verified by luciferase reporter assay and RNA immunoprecipitation assay. Further, hsa_circ_0004370 involved in the up-regulation of LASP1 by sponging miR-1294. Besides, the inhibition of the down-regulated hsa_circ_0004370 on cell malignant behaviors was rescued by miR-1294 inhibitor. Finally, this rescue effect was abrogated by suppressing the expression of LASP1. The results present here suggest that hsa_circ_0004370 functions as an oncogene on cell proliferation, apoptosis, and invasion via miR-1294/LASP1 axis.

<p>Circ_0062020 Knockdown Strengthens the Radiosensitivity of Prostate Cancer Cells</p>

“…Circular RNAs (circRNAs) are a kind of non-coding RNAs, which were documented to be associated with cancers process. [9][10][11][12] The covalent closed loop structure of circRNAs provided more stability due to the lack of poly-A capture, which unleashed the potential of circRNAs used as biomarkers in the diagnosis of cancers. 13 Recently, numerous studies have exposed the momentous role of circRNAs in PCa.…”

Circular RNAs and esophageal cancer

“…Through the action of miRNA sponge, the tumor inhibitory activity of miR-876-5p is weakened and the expression of MAGE-A family of downstream targeted tumor antigens of miR-876-5p is enhanced. It is proved that the overexpression of ciRS-7 promotes the proliferation, migration and invasion of ESCC cells LASP1), suggesting that hsa_circ_0004370 may be an oncogene affecting proliferation, apoptosis and invasion through the miR-1294/LASP1 axis [41]. Another study found that hsa_circ_0000654 was significantly up-regulated in ESCC tissues and cell lines, and its high expression was significantly related to increased T stage and local lymph node metastasis in patients with ESCC.…”

“…It is suggested that the level of plasma circGSK3β may be a valuable predictor of recurrence/metastasis of EC [ 37 ]. Following results showed that the expressions of circ_0000654 [ 38 ], circPVT1 [ 39 ], circ_0006168 [ 40 ], circ_0004370 [ 41 ], circ-TTC17 [ 42 ], circ_0067934 [ 43 ], circ_0006948 [ 44 ], circ_0030018 [ 45 ], circ_100876 [ 46 ], circ-DLG1 [ 47 ] and circ-SLC7A5 [ 48 ] were up-regulated in EC and related to the poor prognosis of EC. However, the other group found that the expression of circ-SMAD7 in EC was significantly down-regulated and negatively correlated with tumor stage and lymph node metastasis [ 49 ], and the low expression of circ-Foxo3 was related to poor prognosis [ 50 ].…”

“…Lost the function of hsa_circ_0004370 by siRNA significantly inhibited the proliferation and invasion of EC cells, and promoted cell apoptosis. Using bioinformatics methods, hsa_circ_0004370 can sponge miR-1294 and indirectly up-regulate the levels of LIM and SH3 domain protein 1 (LASP1), suggesting that hsa_circ_0004370 may be an oncogene affecting proliferation, apoptosis and invasion through the miR-1294/LASP1 axis [ 41 ]. Another study found that hsa_circ_0000654 was significantly up-regulated in ESCC tissues and cell lines, and its high expression was significantly related to increased T stage and local lymph node metastasis in patients with ESCC.…”

<p>Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells</p>

“…7,12 Moreover, circ_0005198 and circ_0004370 can sponge miR-1294 to promote glioma and esophageal cancer progression, respectively. 13,14 However, the function and mechanism of miR-1294 in osteosarcoma are not fully understood and need further investigation.…”