Hsa_circ_0006232 promotes laryngeal squamous cell cancer progression through FUS-mediated EZH2 stabilization

Wu, Tianyi; Wang, Guangke; Zeng, Xiaoyan; Sun, Zhanwei; Li, Shichao; Wang, Weiwei; Yu, Boyu

doi:10.1080/15384101.2021.1959973

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…As a well‐characterized RNA binding protein, FUS was able to bind to the mRNA of many important genes and play important roles based on its target genes. For example, FUS bound to the mRNA of EZH2, the famous core protein of the polycomb repressive complex 2 (PRC2), stabilized the EZH2 mRNA and regulated EZH2‐mediated suppression of PTEN to promote proliferation, migration and invasion of laryngeal squamous cell cancer cells 39 . In this study, we demonstrated that FUS bound to and stabilized the mRNA of the ferroptosis suppressor gene NRF2 and regulated NRF2‐mediated suppression of ferroptosis, thus contributing to pancreatic cancer cell proliferation and invasion.…”

Section: Discussionmentioning

confidence: 99%

Exosomal HSPB1, interacting with FUS protein, suppresses hypoxia‐induced ferroptosis in pancreatic cancer by stabilizing Nrf2 mRNA and repressing P450

Zhang,

Yang,

2024

J Cellular Molecular Medi

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Ferroptosis is a new type of programmed cell death, which has been involved in the progression of tumours. However, the regulatory network of ferroptosis in pancreatic cancer is still largely unknown. Here, using datasets from GEO and TCGA, we screened HSPB1, related to the P450 monooxygenase signalling, a fuel of ferroptosis, to be a candidate gene for regulating pancreatic cancer cell ferroptosis. We found that HSPB1 was enriched in the exosomes derived from human pancreatic cancer cell lines SW1990 and Panc‐1. Then, hypoxic SW1990 cells were incubated with exosomes alone or together with HSPB1 siRNA (si‐HSPB1), and we observed that exosomes promoted cell proliferation and invasion and suppressed ferroptosis, which was reversed by si‐HSPB1. Moreover, we found a potential binding affinity between HSPB1 and FUS, verified their protein interaction by using dual‐colour fluorescence colocalization and co‐IP assays, and demonstrated the promoting effect of FUS on oxidative stress and ferroptosis in hypoxic SW1990 cells. Subsequently, FUS was demonstrated to bind with and stabilize the mRNA of Nrf2, a famous anti‐ferroptosis gene that negatively regulates the level of P450. Furthermore, overexpressing FUS and activating the Nrf2/HO‐1 pathway (using NK‐252) both reversed the inhibitory effect of si‐HSPB1 on exosome functions. Finally, our in vivo studies showed that exosome administration promote tumour growth in nude mice of xenotransplantation, which was able to be eliminated by knockdown of HSPB1. In conclusion, exosomal HSPB1 interacts with the RNA binding protein FUS and decreases FUS‐mediated stability of Nrf2 mRNA, thus suppressing hypoxia‐induced ferroptosis in pancreatic cancer.

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Section: Discussionmentioning

confidence: 99%

Exosomal HSPB1, interacting with FUS protein, suppresses hypoxia‐induced ferroptosis in pancreatic cancer by stabilizing Nrf2 mRNA and repressing P450

Zhang,

Yang,

2024

J Cellular Molecular Medi

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“…On the one hand, FUS binds to circRNAs to further stabilize the mRNA of the downstream target gene. In laryngeal squamous cell cancer, FUS combined with hsa_circ_0006232 to promote the stabilization of EZH2 [ 24 ]. Besides, circ0005276 was found to recruit FUS to increase mRNA stability of XIAP, thereby promoting prostate cancer progression [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

CircHIPK3 relieves vascular calcification via mediating SIRT1/PGC-1α/MFN2 pathway by interacting with FUS

Feng,

Qi,

Xiao

et al. 2023

BMC Cardiovasc Disord

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Background Circular RNAs (circRNAs) have been reported to regulate the biological processes of human diseases. CircHIPK3 has been implicated in vascular calcification, but the downstream regulatory mechanisms remain unclear. Our study aimed to understand the regulatory function of circHIPK3 in vascular calcification. Methods CircHIPK3 expression in atherosclerosis (AS) serum samples and vascular smooth muscle cells (VSMCs) calcification model was assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The binding relationships between fused in sarcoma (FUS) and circHIPK3 or sirtuin 1 (SIRT1) were verified by RNA immunoprecipitation (RIP) assay and RNA pull-down assays. Alkaline phosphatase (ALP) activity and alizarin red staining assays were performed to evaluate the biological effect of β-glycerophosphate (β-GP) and circHIPK3 on calcium deposition. qRT-PCR and western blot assays were used to examine the effect of β-GP, circHIPK3, SIRT1, mitofusin 2 (MFN2), and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) on VSMCs calcification and the expression of calcification-related proteins. Results In AS serum samples and VSMCs calcification model, the expression of circHIPK3 was significantly reduced. CircHIPK3 overexpression inhibited ALP activity and calcium deposition in β-GP-induced VSMCs. Moreover, circHIPK3 could recruit FUS to further stabilize SIRT1 mRNA. CircHIPK3 promoted MFN2 expression to alleviate VSMCs calcification via activating SIRT1/PGC-1α signaling. Conclusion The positive regulation of circHIPK3/FUS/SIRT1/PGC-1α/MFN2 signaling pathway contributed to the alleviate VSMCs calcification, revealing a novel regulatory axis for vascular calcification.

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“…CircANTRL1 may act as a sponge for miR-23a-3p to promote PTEN expression and induce cell cycle arrest, ultimately contributing to improvement of radiosensitivity in OSCC ( 72 ) ( Figure 2 and Table 1 ). Research also showed that silencing hsa_circ_0006232 in LSCC can enhance the expression of PTEN in tumor tissues and affect the process of LSCC ( 73 ) ( Table 1 ).…”

Section: Circrnasmentioning

confidence: 99%

CircRNAs: Roles in regulating head and neck squamous cell carcinoma

et al. 2022

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Head and neck squamous cell carcinoma (HNSCC), the most common head and neck malignant tumor, with only monotherapy, is characterized by poor prognosis, and low 5-year survival rate. Due to the lack of therapeutic targets, the targeted drugs for HNSCC are rare. Therefore, exploring the regulation mechanism of HNSCC and identifying effective therapeutic targets will be beneficial to its treatment of. Circular RNA (CircRNA) is a class of RNA molecules with a circular structure, which is widely expressed in human body. CircRNAs regulate gene expression by exerting the function as a miRNA sponge, thereby mediating the occurrence and development of HNSCC cell proliferation, apoptosis, migration, invasion, and other processes. In addition, circRNAs are also involved in the regulation of tumor sensitivity to chemical drugs and other biological functions. In this review, we systematically listed the functions of circRNAs and explored the regulatory mechanisms of circRNAs in HNSCC from the aspects of tumor growth, cell death, angiogenesis, tumor invasion and metastasis, tumor stem cell regulation, tumor drug resistance, immune escape, and tumor microenvironment. It will assist us in discovering new diagnostic markers and therapeutic targets, while encourage new ideas for the diagnosis and treatment of HNSCC.

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Hsa_circ_0006232 promotes laryngeal squamous cell cancer progression through FUS-mediated EZH2 stabilization

Cited by 8 publications

References 30 publications

Exosomal HSPB1, interacting with FUS protein, suppresses hypoxia‐induced ferroptosis in pancreatic cancer by stabilizing Nrf2 mRNA and repressing P450

Exosomal HSPB1, interacting with FUS protein, suppresses hypoxia‐induced ferroptosis in pancreatic cancer by stabilizing Nrf2 mRNA and repressing P450

CircHIPK3 relieves vascular calcification via mediating SIRT1/PGC-1α/MFN2 pathway by interacting with FUS

CircRNAs: Roles in regulating head and neck squamous cell carcinoma

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