Hsa_circ_0021727 (circ-CD44) promotes ESCC progression by targeting miR-23b-5p to activate the TAB1/NFκB pathway

Fan, Meng; Zhang, Xiaokang; Wang, Yanting; Lin, Jie; Tang, Yulin; Zhang, Guisheng; Qiu, Binqiang; Zeng, Xingdu; Liu, Weiyou; He, Xin

doi:10.1038/s41419-022-05541-x

Cited by 11 publications

(6 citation statements)

References 66 publications

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“…The significant transcriptomes associated with ESCC have been widely investigated through high-throughput approaches. For example, known ESCC-related miRNAs include miR-23b-5p, miR-877-3p, and miR-17-92, among others (13)(14)(15)(16). Several published works demonstrated that chemotherapy resistance can be affected by mRNAs like TUG1, ZFX, and NRF2, and miRNAs like miR-130a-3p, miR-125a-5p, and miR-224 (17)(18)(19)(20)(21).…”

Section: Discussionmentioning

confidence: 99%

Use of exosome transcriptome-based analysis to identify novel biomarkers in patients with locally advanced esophageal squamous cell carcinoma undergoing neoadjuvant chemoradiotherapy

Yang¹,

Zhang²,

Liu³

et al. 2023

Ann Transl Med

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Background: The prognosis of esophageal squamous cell carcinoma (ESCC) is improved by neoadjuvant chemoradiotherapy (nCRT), especially for patients with pathologic complete response (pCR). Despite the efforts to predict treatment response using multimodality, no molecule has proven to be a strong biomarker.This study aimed to profile the expression of exosome transcriptome that could predict pCR in ESCC before and after nCRT.Methods: We collected paired blood samples of 15 patients with ESCC who received nCRT and radical surgery. They were divided into 3 groups: (A) residual tumor in the first clinical response evaluation (CRE-1), (B) no residual tumor in CRE-1 but with residual tumor in CRE-2 which was performed after 5-6 weeks, and (C) no residual tumor in CRE-1 or CRE-2. For each patient, the blood sample was collected before nCRT (time point 0); and then 6 weeks after nCRT, the clinical response was evaluated, and another blood sample was collected (time point 1).Results: Using the intersection of different sets, we found 23 progression-associated messenger RNAs (mRNAs) and 67 remission-associated mRNAs. Between remission-associated mRNAs and the targets of progression-associated (carcinogenic) microRNAs (miRNAs), the intersection was acquired, and 2 miRNA-mRNA networks (IFIT2-miR-3615-IFIT2-miR-484 and BTN3A3-miR-6803-3p) were identified. Among the intersection of progression-associated (carcinogenic) mRNAs and the targets of remission-associated miRNAs, there is a network with miR-132-3p (remission-associated miRNA) located at the core, matched with DICER1, KLHL8, ANKRD12, ASH1L, and IMP4.Conclusions: Our findings identified altered plasma exosome RNAs among the different groups and between different time points of nCRT, as well as the corresponding enrichments and regulatory networks, which may serve as potentially predictors of treatment response for patients with ESCC after nCRT.

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Section: Discussionmentioning

confidence: 99%

Use of exosome transcriptome-based analysis to identify novel biomarkers in patients with locally advanced esophageal squamous cell carcinoma undergoing neoadjuvant chemoradiotherapy

Yang¹,

Zhang²,

Liu³

et al. 2023

Ann Transl Med

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“…Numerous studies have shown that circRNAs can participate in the progression of cancer by regulating the NF-κB pathway. 27,28 For example, circWDR37 deficiency inhibits chemotherapy-induced proliferation and metastasis of senescent NPC cells in vitro by inhibiting the NF-κB pathway. 27 We found that circRNA_CDKN1A acts by affecting the NF-κB pathway.…”

Section: Knockdown Of Circrna_cdkn1a Activates the Nf-κb Pathwaymentioning

confidence: 99%

Downregulated circRNA_CDKN1A promotes gallbladder cancer progression through activation of the NF‐κB pathway

Hu,

Yang,

Wang

et al. 2024

Cell Biochemistry & Function

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This study uncovered the potential clinical value and molecular driving mechanisms of circular RNAs (circRNAs) in gallbladder cancer (GBC). Differentially expressed circRNAs in GBC cells were screened by high‐throughput sequencing. CircRNA_CDKN1A (circBase ID: hsa_circ_0076194) was knocked out in BGC‐SD cells through transfection with sh‐circRNA_CDKN1A. Then, proliferation was investigated via CCK8 and EdU assays, apoptosis via flow cytometry, migration via wound healing assays, and invasion via Transwell assays. Bioinformatics analysis of circRNA_CDKN1A‐related signaling pathways was performed using MetScape and g:Profiler. Results showed that the knockdown of circRNA_CDKN1A enhanced the proliferation, migration, and invasion of GBC cells and inhibited apoptosis. In addition, knocking out circRNA_CDKN1A promoted GBC cell proliferation and enhanced the dry indices of the OCT4 protein and CD34 expression levels. The knockdown of circRNA_CDKN1A activated the epithelial–mesenchymal transition pathway. Bioinformatics analysis revealed that the biological role of circRNA_CDKN1A in GBC cells involved the NF‐κB pathway. LY2409881, which is an NF‐κB inhibitor, reversed the effects induced by the knockdown of circRNA_CDKN1A in GBC‐SD cells. In summary, the knockdown of circRNA_CDKN1A promoted the progression of GBC by activating the NF‐κB signaling pathway. For the first time, this study revealed the mechanism of circRNA_CDKN1A‐mediated regulatory action in GBC and identified the newly discovered circRNA_CDKN1A–NF‐κB signaling axis as a potentially important candidate for clinical therapy and prognostic diagnosis of GBC.

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“…Insulin-like growth factor 2 (IGF2BP2) mediates circRUNX1-enhanced FOXP3 expression by acting as a competitive miRNA sponge to inhibit miR-449b-5p activity, thus promoting ESCC cell proliferation and metastasis in vitro and in vivo (53). circCD44 functions as a sponge for miR-23b-5p, activating TGF-β-activated kinase 1/NF-κB signaling and promoting ESCC cell migration, invasion and proliferation (54). circ-0026611 is highly expressed in ESCC cells and exosomes, and exosomal circ-0026611 promotes ESCC lymphangiogenesis by interacting with N-α-acetyltransferase 10 and inhibiting prospero homeobox 1 acetylation and ubiquitination (55).…”

Section: Biological Function and Clinical Significance Of Circrnas In...mentioning

confidence: 99%

Biological functions of circRNA in regulating the hallmarks of gastrointestinal cancer (Review)

Qiu,

Chen,

Zeng

2024

Int J Oncol

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Circular RNA (circRNA) was first observed in the cytoplasm of eukaryotic cells in 1979, but it was not characterized in detail until 2012, when high-throughput sequencing technology was more advanced and available. Consequently, the mechanism of circRNA formation and its biological function have been progressively elucidated by researchers. circRNA is abundant in eukaryotic cells and exhibits a certain degree of organization, timing and disease-specificity. Additionally, it is poorly degradable, meeting the characteristics of an ideal clinical biomarker. In the present review, the recent research progress of circRNAs in digestive tract malignant tumors was primarily discussed. This included the roles, biological functions and clinical significance of circRNA, providing references for its research value and clinical potential in gastrointestinal cancer. Contents1. Introduction 2. Biogenesis of circRNA 3. Biological function of circRNA 4. Biological function and clinical significance of circRNAs in gastrointestinal cancer 5. Bioinformatics screening and functional analysis of circRNAs in digestive tumors 6. Conclusion

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Hsa_circ_0021727 (circ-CD44) promotes ESCC progression by targeting miR-23b-5p to activate the TAB1/NFκB pathway

Cited by 11 publications

References 66 publications

Use of exosome transcriptome-based analysis to identify novel biomarkers in patients with locally advanced esophageal squamous cell carcinoma undergoing neoadjuvant chemoradiotherapy

Use of exosome transcriptome-based analysis to identify novel biomarkers in patients with locally advanced esophageal squamous cell carcinoma undergoing neoadjuvant chemoradiotherapy

Downregulated circRNA_CDKN1A promotes gallbladder cancer progression through activation of the NF‐κB pathway

Biological functions of circRNA in regulating the hallmarks of gastrointestinal cancer (Review)

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