Hsa_circ_101555 functions as a competing endogenous RNA of miR-597-5p to promote colorectal cancer progression

Chen, Zhenlong; Ren, Rui; Wan, Daiwei; Wang, Yilin; Xue, Xiaofeng; Jiang, Min; Shen, Jiaqing; Han, Yongtao; Liu, Fei; Shi, Jian-Quan; Kuang, Yuting; Li, Wei; Zhi, Qiaoming

doi:10.1038/s41388-019-0857-8

Cited by 80 publications

(82 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 In CRC, miR-597-5p was verified as the binding target of circ101555 and promotes tumor growth by regulating CSNK1G1. 33 To better explore the molecular mechanism of circRUNX1, we used bioinformatics algorithms to predict the miRNA-binding sites. Among these candidates, miR-145-5p was selected as the downstream target of circRUNX1.…”

Section: Discussionmentioning

confidence: 99%

Elevated Levels of circRUNX1 in Colorectal Cancer Promote Cell Growth and Metastasis via miR-145-5p/IGF1 Signalling

Chen¹,

Li²,

Ye³

et al. 2020

OTT

View full text Add to dashboard Cite

Background: Emerging evidence suggests that circular RNAs (circRNAs) are vital regulators in a range of cancers. "miRNA sponge" is the most reported role played by circRNAs in many tumors. The insulin-like growth factor (IGF) 1 pathway plays a key role in the development and progression of many cancers, including colorectal cancer (CRC). The aim of the study is to establish the potential clinical value and driving molecular mechanisms of circRNAs in CRC. Materials and Methods: Real-time quantitative RT-PCR (qRT-PCR) was performed to measure the circRUNX1 expression in 52 tissue samples from CRC patients. We verified the tumor promotor role of circRUNX1 in cell-based in vitro and in vivo assays. Human growth factor array was used to identify circRUNX1-regulated signaling pathways. We then used a double luciferase reporter assay and RNA fluorescence in situ hybridization to identify the downstream miR-145-5p of circRUNX1. Furthermore, we performed Western blotting and biological function assays to demonstrate if the circRUNX1/miR-145-5p/IGF1 axis is responsible for the proliferation of CRC cells and promotes CRC development. Results: By performing qRT-PCR from CRC tissues and paired adjacent normal mucosa tissues, we identified that circRUNX1 expression was significantly upregulated in CRC tissues and positively related with lymph node metastasis, distant metastasis and advanced tumor-node-metastasis tumor stage in patients. Functionally, circRUNX1 knockdown inhibited cell proliferation and migration and promoted apoptosis, whereas its overexpression exerted opposite effects. In vivo, circRUNX1 promoted tumor growth and metastasis. Mechanically, circRUNX1 shared miRNA response elements with IGF1. circRUNX1 competitively bound to miR-145-5p and prevented miR-145-5p from decreasing the expression of IGF1, which facilitated tumor growth. Conclusion: Our studies verified that circRUNX1 functions as a tumor promotor in CRC cells by targeting the miR-145-5p/IGF1 signaling pathway and may have potential use as a prognostic indicator and therapeutic target in CRC patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Elevated Levels of circRUNX1 in Colorectal Cancer Promote Cell Growth and Metastasis via miR-145-5p/IGF1 Signalling

Chen¹,

Li²,

Ye³

et al. 2020

OTT

View full text Add to dashboard Cite

show abstract

“…6 Chen et al documented that circ_101555 was elevated in CRC, and its depletion blocked cell proliferation and facilitated apoptosis. 7 Also, circ_0007142, a novel circRNA originated from linear RNA DOCK1, has been documented to be dysregulated in CRC. However, the mechanism of circ_0007142 is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p

Yin¹,

Xu²,

Li³

et al. 2020

OTT

View full text Add to dashboard Cite

Background: Colorectal cancer (CRC) is a common malignant tumor in digestive system. Circular RNA (circRNA) circ_0007142 has been identified as an oncogene in CRC. However, the mechanism of circ_0007142 in CRC was rarely reported. Materials and Methods: The levels of circ_0007142, dedicator of cytokinesis 1 (DOCK1), microRNA-122-5p (miR-122-5p), and cell division cycle 25A (CDC25A) in CRC tissues (n=31) and cells were examined by quantitative real-time polymerase chain reaction (qRT-PCR). The cell viability and colony-forming ability were evaluated via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and colony-formation assay, respectively. The migrated and invaded abilities were monitored by Transwell assay. The dual-luciferase reporter assay was performed to validate the interactions between miR-122-5p and circ_0007142 or CDC25A. The protein level of CDC25A was detected via Western blot assay. The biological role of circ_0007142 was examined by xenograft tumor model in vivo. Results: The levels of circ_0007142 and CDC25A were enhanced and the level of miR-122-5p was declined in CRC tissues and cells, while the level of DOCK1 had no fluctuation. Circ_0007142 sponged miR-122-5p and CDC25A was a target of miR-122-5p. Circ_0007142 knockdown impeded cell proliferation, colony formation, migration, and invasion in CRC cells by regulating miR-122-5p. Besides, miR-122-5p inhibitor promoted cell proliferation, colony formation, migration, and invasion in CRC cells by modulating CDC25A. Circ_0007142 regulated CDC25A expression in CRC cells by sponging miR-122-5p. Moreover, circ_0007142 knockdown blocked CRC tumor growth in vivo. Conclusion: Circ_0007142 modulated CDC25A expression to promote CRC progression by sponging miR-122-5p.

show abstract

“…For instance, overexpressed circRNAs are linked to chemokine signaling which can directly promote the progression of tumor by inducing the proliferation of cancer cells and preventing their apoptosis (38). To further understand the mechanisms of the circRNAs, we carefully searched and read the relevant studies, and found that circRNAs may function as miRNA sponges or potent competitive endogenous RNA (ceRNA) molecules to regulate gene expression (39)(40)(41). In this study, we predicted the potential miRNA targets of circ-PNN through miRanda and RNAhybrid database.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Profiling of Serum Exosomal Circular RNAs Reveals Circ-PNN as a Potential Biomarker for Human Colorectal Cancer

Xie

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are emerging as cardinal areas of focus in the non-coding RNA field. Growing evidences have revealed exosomal circRNAs as potential biomarkers for detection of various cancers. However, the clinical importance of most serum exosomal circRNAs in colorectal cancer (CRC) have rarely been investigated. In this study, we examined the possible clinical application of serum exosomal circRNAs in the diagnosis of CRC. Firstly, we conducted RNA sequencing (RNA-seq) analysis using fifty CRC and fifty healthy control serum samples to identify CRC-related circRNAs. The sequencing data showed 122 differentially expressed circRNAs including 100 up-regulated and 22 down-regulated circRNA transcripts in CRC patients. Then, eight most dysregulated circRNAs were selected for validation by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay. Validation analysis revealed that the serum exosomal circ-PNN (hsa_circ_0101802) levels were significantly up-regulated in CRC cases compared with those in the healthy control groups. Receiver operating characteristic curve (ROC) analysis suggested that circ-PNN had significant value in CRC diagnosis with areas under the ROC curve (AUC) of 0.855 and 0.826 in the training and validation sets, respectively. We also found that the AUC of serum exosomal circ-PNN for early-stage CRC was 0.854. Finally, a network map based on circ-PNN was constructed to determine its potential miRNA-mRNAs binding. We also demonstrated that the expression of hsa-miR-6833-3P, hsa-let-7i-3p and hsa-miR-1301-3P were negatively correlated with circ-PNN in CRC patients. Collectively, our findings indicated that serum exosomal circ-PNN might be a potential non-invasive biomarker for the detection of CRC and may play a crucial role in the pathogenesis of CRC.

show abstract

Hsa_circ_101555 functions as a competing endogenous RNA of miR-597-5p to promote colorectal cancer progression

Cited by 80 publications

References 39 publications

<p>Elevated Levels of circRUNX1 in Colorectal Cancer Promote Cell Growth and Metastasis via miR-145-5p/IGF1 Signalling</p>

<p>Elevated Levels of circRUNX1 in Colorectal Cancer Promote Cell Growth and Metastasis via miR-145-5p/IGF1 Signalling</p>

<p>Circular RNA circ_0007142 Facilitates Colorectal Cancer Progression by Modulating CDC25A Expression via miR-122-5p</p>

RNA-Seq Profiling of Serum Exosomal Circular RNAs Reveals Circ-PNN as a Potential Biomarker for Human Colorectal Cancer

Contact Info

Product

Resources

About