hsDNA groove binding, photocatalytic activity, and in vitro breast and colon cancer cell reducing function of greener SeNPs

Abstract

Selenium nanoparticles (SeNPs) have attracted great attention because of their superior optical properties and wide utilization in biological and biomedical studies. This paper reports an environmentally benign procedure of greener monodispersible SeNP synthesis using the reducing power of Trigonella foenum-graecum extract, characterization and their protective effect against unfolded (Herring sperm DNA) hsDNA. We investigated the anti-cancer activity of SeNPs against MCF-7, MDA MB 435 and COLO-205 cells. The photocatalytic activity of SeNPs was investigated for the degradation of a Sunset Yellow FCF (SYFCF) dye using ultraviolet-B light. The reduction of the Se ion to SeNPs was monitored by ultraviolet-visible spectroscopy (UV-vis). The size and morphology of the SeNPs were characterized by high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), and Dynamic Light Scattering (DLS). The SeNPs were stable, and the diameter was homogeneous at around 5-12 nm. Interactions of various concentrations of SeNPs with hsDNA were systematically investigated by UV-vis, fluorescence, circular dichroism (CD), polarimetry and FTIR spectroscopy under physiological conditions. The results from fluorescence spectroscopy indicated that SeNPs quenched the fluorescence intensity of hsDNA with increasing concentrations. The modified Stern-Volmer quenching rate constant Ksv, binding constant K and binding sites n at different temperatures and the corresponding thermodynamic parameters ΔH°, ΔG° and ΔS° were calculated. Hoechst 33258 and methyl green (MG) site markers, melting experiment (Tm), viscosity measurements and sequence specificity verification by DNA bases clarified that SeNPs bind to hsDNA via a groove site. The rate of photocatalytic degradation of the SYFCF dye in the presence and absence of photocatalysts (SeNPs) was studied using UV-vis, the results showed appreciable degradation of the SYFCF dye. Our results suggested that nano Se can be used as a promising selenium species with potential application in cancer treatment. These nanoparticles were found to be the most active cytotoxic agent prepared in a new green synthesis manner, causing >50% inhibition of MCF-7, MDA MB-435 and COLO-205 cell proliferation at concentrations <10(-7) M. Hence these SeNPs could be recognized as promising materials for biomedical applications.

Selenium nanoparticles: a review on synthesis and biomedical applications

“…To overcome this unavoidable impact, chemoradiotherapy can be utilized with a radioactive seed to deliver enhanced, efficient and precise radiation. Based on this concept, Bhattacharjee et al 205 investigated a combined in-vivo and in-vitro experiment by utilizing folic acid capped SeNPs (FA-SeNPs) in conjugation with radioactive 125 I seeds to impart enhanced anticancer viability. In comparison with individual treatment, the blend of (FA-SeNPs) and 125 I seeds demonstrated better in-vivo antitumor and lower cytotoxicity in MCF-7 infected mice.…”

“…Based on this concept, Bhattacharjee et al 205 investigated a combined in-vivo and in-vitro experiment by utilizing folic acid capped SeNPs (FA-SeNPs) in conjugation with radioactive 125 I seeds to impart enhanced anticancer viability. In comparison with individual treatment, the blend of (FA-SeNPs) and 125 I seeds demonstrated better in-vivo antitumor and lower cytotoxicity in MCF-7 infected mice. Yu et al 209 reported that Se nanoparticles in combination with radiation reduces the damage to nearby tissues and enhances the carcinogenic cell affectability to harmful impacts of illumination on breast cancer cell MCF-7 by in-vitro study.…”

One Pot Bio-Synthesis of Palladium Nanoneedles and Its Bioavailability

“…Binding mechanism gives (S3) binding constant (K) between BSA and PdNNs was found in the range of 10 4 M − 1 and number of binding sites (n) between BSA and PdNNs was approximately equal to 1 delineated in Table S3. Which suggested that PdNNs can easily be complex in protein as well as released in desired target areas and shows moderate a nity [52,53].…”

Deciphering the sensing of α-amyrin acetate with hs-DNA: a multipronged biological probe

“…Interaction between a-AA and DNA was determined by UV-Vis absorption, uorescence measurements, CD measurements, viscosity experiments; thermal denaturation and minor groove displacement assays were demonstrated by a previously reported method. 27 Glide version 5.7 -Schrödinger suite 2013-1 was used for the molecular docking of a-AA-DNA binding. The B-DNA dodecamer d(CGCGAATTCGCG) 2 (PDB ID: 1BNA) structure was copied from the protein data bank (http://www.rcsb.org./pdb).…”