HSF3, a new heat shock factor from Arabidopsis thaliana, derepresses the heat shock response and confers thermotolerance when overexpressed in transgenic plants

Prändl, Ralf; Hinderhofer, Katrin; Eggers-Schumacher, G.; Schöffl, Fritz

doi:10.1007/s004380050731

Cited by 169 publications

(134 citation statements)

References 38 publications

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“…Thus, the higher number of plant HSFs could potentially increase the complexity of interactions with other transcription factors and provide additional functional diversification by evolving distinct specific expression patterns (as demonstrated in this work for HaHSFA9). Previous evidence supporting functional specialization of plant HSFs is scarce and connected only to thermotolerance (24,25) or to cell death defense (26). This was deduced from the specific effects observed by gain of function (24) or, more recently, by loss of function of different HSFs (25,26).…”

Section: Discussionmentioning

confidence: 99%

A Seed-specific Heat-shock Transcription Factor Involved in Developmental Regulation during Embryogenesis in Sunflower

Almoguera

Rojas²,

Díaz‐Martín³

et al. 2002

Journal of Biological Chemistry

115

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We report the cloning and functional characterization of the first heat-shock transcription factor that is specifically expressed during embryogenesis in the absence of environmental stress. In sunflower embryos this factor, HaHSFA9, trans-activated promoters with poor consensus heat-shock cis-elements, including that of the seed-specific Hahsp17.6G1 gene. Mutations that improved the heat-shock cis-element consensus at the Hahsp17.7G4 promoter impaired transient activation by HaHSFA9 in sunflower embryos. The same mutations did not affect heat-shock-induced gene expression of this promoter in transgenic tobacco plants but reduced the developmental activation by endogenous heat-shock transcription factors (HSFs) in seeds. Sunflower, and perhaps other plants such as tobacco, differs from the vertebrate animal systems in having at least one specialized HSF with expression and (or) activation patterns strictly restricted to embryos. Our results strongly indicate that HaHSFA9 is a transcription factor critically involved in the developmental activation of Hahsp17.6G1 and in that of similar target genes as Hahsp17.7G4.In eukaryotes, the heat-shock response and some developmental processes are under the control of a family of conserved DNA-binding proteins known as the heat-shock transcription factors (HSFs). 1 Although in some systems, as in Drosophila melanogaster, this regulation involves a single HSF (1), multigenic families of HSFs participate in vertebrate and in plant systems. These families have different sizes, which, together with particular gene expression and activation patterns for the HSFs, might have consequences in the degree of overlapping of regulatory functions mediated by these factors. The specific role of the different HSFs is mostly unknown, particularly for the plant HSFs, and for involvement in developmental processes, as the regulation of gene expression during embryogenesis (See for example, Ref. 2 and the reviews in Refs. 3 and 4).In vertebrate systems, three different HSFs (HSF1, HSF2, and HSF3) have ubiquitous expression patterns (for example, Refs. 5 and 6 and the review in Ref.3). A fourth HSF found in humans displays tissue-specific expression patterns, which suggested specialized functions but not related to embryogenesis (HSF4, Ref. 7). Plants contain the highest number of HSF genes in eukaryotes. This is inferred from in silico analyses from the fully sequenced Arabidopsis thaliana model and from functional analyses of different cloned HSFs in tomato, Arabidopsis, and other plants (reviewed in Refs. 4 and 8 and references therein). Plant HSFs share unique structural and phylogenetic relationships compared with the vertebrate HSFs (9). Fifteen of the 21 putative HSFs from A. thaliana thus contain an insertion of 21 amino acid residues in the oligomerization domain (characteristic of the plant class A HSFs), whereas class B HSFs have no such insertion. Gene expression studies for plant HSFs are very scarce, with only fragmentary data at the mRNA level and even scarcer reports for prote...

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Section: Discussionmentioning

confidence: 99%

A Seed-specific Heat-shock Transcription Factor Involved in Developmental Regulation during Embryogenesis in Sunflower

Almoguera

Rojas²,

Díaz‐Martín³

et al. 2002

Journal of Biological Chemistry

115

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“…Efficient HSF binding requires at least three alternating units (5 0 -nGAAnnTTCnnGAAn-3 0 ) [42]. In spite of their conserved transactivation function upon heat shock, HSFs show differences in induction threshold [43] and regulation of the heat response [44], which could provide diverse induction profiles for target genes under various stress conditions. Plant HSFs are distinct compared with HSFs from other organisms.…”

Section: Reviewmentioning

confidence: 99%

Acquired tolerance to temperature extremes

Sung

Kaplan

Lee

et al. 2003

Trends in Plant Science

515

334

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“…In fact, there are several reports describing high temperature tolerance conferred by Hsfs (Lee et al 1995;Liu et al 2009;Lohmann et al 2004;Mishra et al 2002;Ogawa et al 2007;Prändl et al 1998;Yokotani et al 2007). Higher plants contain coding sequences for many more Hsfs in their genomes than do animals or Chlamydomonas.…”

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confidence: 99%

Generation of transgenic rice expressing heat shock protein genes under cool conditions

Yasuda

Sagehashi

Shimosaka

et al. 2013

Plant Biotechnology

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The heat shock response of rice, including expression of heat shock transcription factors (Hsfs), was investigated to elucidate the molecular regulation of its high-temperature tolerance. In silico analysis revealed that the rice genome encodes more than 19 species of Hsf genes that can be organized into three classes, A, B, and C. Rice seedlings treated with high temperature, express three class A Hsf genes (HsfA2a, HsfA2c, and HsfA2d) and two class B Hsf genes (HsfB2b and HsfB2c) at significantly elevated levels. Transgenic rice plants overexpressing these three class A Hsf genes controlled by the rice actin 1 promoter or the wheat cold response (WCR) promoter expressed the transgenes, but did not express heat shock response genes such as small HSPs, whose expression is controlled by Hsfs. Treatment with geldanamycin, an inhibitor of HSP90, elevated the expression of HSPs in the WCR::Hsf transformant. Therefore, transgenic rice co-expressing WCR::Hsfs and a dominant negative HSP90 mutant were generated in which the heat shock response could be induced under WCR promoter-activating conditions. Successful induction of the heat shock response under cool conditions in this co-expression line suggests that HSP90 controls the heat shock response via the activity of Hsf in rice cells.

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HSF3, a new heat shock factor from Arabidopsis thaliana, derepresses the heat shock response and confers thermotolerance when overexpressed in transgenic plants

Cited by 169 publications

References 38 publications

A Seed-specific Heat-shock Transcription Factor Involved in Developmental Regulation during Embryogenesis in Sunflower

A Seed-specific Heat-shock Transcription Factor Involved in Developmental Regulation during Embryogenesis in Sunflower

Acquired tolerance to temperature extremes

Generation of transgenic rice expressing heat shock protein genes under cool conditions

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