HSM2 (HMO1) gene participates in mutagenesis control in yeast Saccharomyces cerevisiae

Alekseev, Sergey; Ковальцова, С В; Федорова, И. В.; Gracheva, L.M.; Evstukhina, Tatiana A.; Peshekhonov, V. T.; Korolev, V. G.

doi:10.1016/s1568-7864(02)00005-8

Cited by 24 publications

(9 citation statements)

References 19 publications

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“…As mentioned above, upregulation of SMC4 indicates an increased need for chromosome organization and maintenance in response to shear. These data are in agreement with the finding that strains containing deletions in the genes VIK1, involved in monitoring chromatid cohesion, and HSM2, which is involved in genome maintenance, show a significant survival disadvantage in response to increased shear (Alekseev et al, 2002;Mayer et al, 2004).…”

Section: Discussionsupporting

confidence: 92%

Gene expression and survival changes in Saccharomyces cerevisiae during suspension culture

Johanson

Allen

González-Villalobos

et al. 2006

Biotech & Bioengineering

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This study explores the connection between changes in gene expression and the genes that determine strain survival during suspension culture, using the model eukaryotic organism, Saccharomyces cerevisiae. The Saccharomyces cerevisiae homozygous diploid deletion pool (HDDP), and the BY4743 parental strain were grown for 18 h in a rotating wall vessel (RWV), a suspension culture device optimized to minimize the delivered shear. In addition to the reduced shear conditions, the RWVs were also placed in a static position or in a shaker in order to change the amount of shear stress on the cells. Using simple linear regression, it was found that there were 140 differentially expressed genes for which >70% of the variation can be explained by shear stress alone. A significant number of these genes are involved in catalytic activity. In the HDDP, shear stress was associated with significant survival changes in 15 deletion strains (R(2>) > 0.7) Interestingly, both analyses uncovered changes in the ribosomal protein machinery. Comparing the changes in gene expression and strain survival under the different shear conditions allows for the insights into the molecular mechanisms behind the cells response to shear stress. This in turn can provide information for the optimization of suspension culture.

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Section: Discussionsupporting

confidence: 92%

Gene expression and survival changes in Saccharomyces cerevisiae during suspension culture

Johanson

Allen

González-Villalobos

et al. 2006

Biotech & Bioengineering

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“…Coating of ssDNA gaps with RPA facilitates the recruitment of the Rad18 ubiquitin ligase (Davies et al , ), which together with the Rad6 ubiquitin conjugating enzyme and the Rad5‐Mms2‐Ubc13 ubiquitylation complex, induces PCNA mono‐ and polyubiquitylation (Hoege et al , ) and mediates postreplicative DDT (Daigaku et al , ; Karras & Jentsch, ). The overlap between Hmo1 and Rfa1 clusters in MMS‐treated cells (Supplementary Fig S1A), together with previous reports indicating a role for Hmo1 in the control of mutagenesis (Alekseev et al , ; Kim & Livingston, ), prompted us to investigate a possible involvement of Hmo1 in DDT and the metabolism of DNA structures arising during recombination‐mediated damage‐bypass.…”

Section: Resultsmentioning

confidence: 86%

“…The Saccharomyces cerevisiae HMGB protein, Hmo1 ‐ the closest ortholog of HMGB1 in yeast‐, shows synthetic lethal interactions with top3 Δ (Gadal et al , ), and binds with preference to single stranded (ss) DNA and to DNA with altered conformations, showing reduced DNA sequence specificity (Kamau et al , ; Bauerle et al , ; Xiao et al , ). In addition, in hmo1 mutant cells, spontaneous and damage‐induced mutagenesis is increased (Alekseev et al , ; Kim & Livingston, , ), suggesting a possible role for Hmo1 in DDT or its regulation. It is of note that while mutation rates vary along chromosomes and correlate with replication timing (Lang & Murray, ), the underlying mechanisms accounting for the preferred usage of error‐free DDT early in S phase remain elusive.…”

Section: Introductionmentioning

confidence: 99%

DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity

et al. 2014

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DNA replication is sensitive to damage in the template. To bypass lesions and complete replication, cells activate recombination-mediated (error-free) and translesion synthesis-mediated (error-prone) DNA damage tolerance pathways. Crucial for error-free DNA damage tolerance is template switching, which depends on the formation and resolution of damage-bypass intermediates consisting of sister chromatid junctions. Here we show that a chromatin architectural pathway involving the high mobility group box protein Hmo1 channels replication-associated lesions into the error-free DNA damage tolerance pathway mediated by Rad5 and PCNA polyubiquitylation, while preventing mutagenic bypass and toxic recombination. In the process of template switching, Hmo1 also promotes sister chromatid junction formation predominantly during replication. Its C-terminal tail, implicated in chromatin bending, facilitates the formation of catenations/hemicatenations and mediates the roles of Hmo1 in DNA damage tolerance pathway choice and sister chromatid junction formation. Together, the results suggest that replication-associated topological changes involving the molecular DNA bender, Hmo1, set the stage for dedicated repair reactions that limit errors during replication and impact on genome stability.

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“…Strains lacking Hmo1 show a decreased growth rate, higher rates of plasmid loss, and increased sensitivity of chromatin to micrococcal nuclease treatment (23). Hmo1 interacts genetically and physically with FKBP12, a conserved prolyl isomerase (8), and it also plays a role in mutagenesis control (2). Hmo1 also plays a role in transcription of the rRNA (9).…”

mentioning

confidence: 99%

An HMG Protein, Hmo1, Associates with Promoters of Many Ribosomal Protein Genes and throughout the rRNA Gene Locus in Saccharomyces cerevisiae

Hall

Wade

Struhl

2006

Molecular and Cellular Biology

139

213

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HMG proteins are architectural proteins that bind to DNA with low sequence specificity, but little is known about their genomic location and biological functions. Saccharomyces cerevisiae encodes 10 HMG proteins, including Hmo1, which is important for maximal transcription of rRNA. Here we use chromatin immunoprecipitation coupled with microarray analysis to determine the genome-wide association of Hmo1. Unexpectedly, Hmo1 binds strongly to the promoters of most ribosomal protein (RP) genes and to a number of other specific genomic locations. Hmo1 binding to RP promoters requires Rap1 and (to a lesser extent) Fhl1, proteins that also associate with RP promoters. Hmo1, like Fhl1 and Ifh1, typically associates with an IFHL motif in RP promoters, but deletion of the IFHL motif has a very modest effect on Hmo1 binding. Surprisingly, loss of Hmo1 abolishes binding of Fhl1 and Ifh1 to RP promoters but does not significantly affect the level of transcriptional activity. These results suggest that Hmo1 is required for the assembly of transcription factor complexes containing Fhl1 and Ifh1 at RP promoters and that proteins other than Fhl1 and Ifh1 also play an important role in RP transcription. Lastly, like mammalian UBF, Hmo1 associates at many locations throughout the rRNA gene locus, and it is important for processing of rRNA in addition to its role in rRNA transcription. We speculate that Hmo1 has a role in coordinating the transcription of rRNA and RP genes.

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HSM2 (HMO1) gene participates in mutagenesis control in yeast Saccharomyces cerevisiae

Cited by 24 publications

References 19 publications

Gene expression and survival changes in Saccharomyces cerevisiae during suspension culture

Gene expression and survival changes in Saccharomyces cerevisiae during suspension culture

DNA bending facilitates the error-free DNA damage tolerance pathway and upholds genome integrity

An HMG Protein, Hmo1, Associates with Promoters of Many Ribosomal Protein Genes and throughout the rRNA Gene Locus in Saccharomyces cerevisiae

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