Hsp: Evolved and Conserved Proteins, Structure and Sequence Studies

NS, Desai; AA, Agarwal; SS, Uplap

doi:10.9735/0975-3087.2.2.67-87

Cited by 11 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of the HSP 70 gene significantly increased under stressors (As, NH 3 , As+NH 3 , and As+NH 3 +T), leading to diminished protein protection from oxidative damage, enhanced ROS production, protein misfolding, and exacerbated oxidative stress ( 23 ). Conversely, dietary Fe-NPs at 15 mg kg -1 diet downregulated HSP 70 gene expression, protecting against proteasomal degradation ( 41 ), promoting proper protein folding, preventing protein aggregation, degrading denatured proteins, and folding misfolded proteins ( 42 ). Surprisingly, dietary Fe-NPs downregulated the HSP 70 gene expression, and this could be due to activation of the HSP transcription factor, which reduces the binding activity of heat shock elements ( 35 , 43 ).…”

Section: Discussionmentioning

confidence: 99%

Unraveling gene regulation mechanisms in fish: insights into multistress responses and mitigation through iron nanoparticles

Kumar,

Thorat,

Gunaware

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

The recent trend of global warming poses a significant threat to ecosystems worldwide. This global climate change has also impacted the pollution levels in aquatic ecosystems, subsequently affecting human health. To address these issues, an experiment was conducted to investigate the mitigating effects of iron nanoparticles (Fe-NPs) on arsenic and ammonia toxicity as well as high temperature stress (As+NH3+T). Fe-NPs were biologically synthesized using fish waste and incorporated into feed formulations at 10, 15, and 20 mg kg-1 diet. A total of 12 treatments were designed in triplicate following a completely randomized design involving 540 fish. Fe-NPs at 15 mg kg-1 diet notably reduced the cortisol levels in fish exposed to multiple stressors. The gene expressions of HSP 70, DNA damage-inducible protein (DDIP), and DNA damage were upregulated by stressors (As+NH3+T) and downregulated by Fe-NPs. Apoptotic genes (Cas 3a and 3b) and detoxifying genes (CYP 450), metallothionein (MT), and inducible nitric oxide synthase (iNOS) were downregulated by Fe-NPs at 15 mg kg-1 diet in fish subjected to As+NH3+T stress. Immune-related genes such as tumor necrosis factor (TNFα), immunoglobulin (Ig), and interleukin (IL) were upregulated by Fe-NPs, indicating enhanced immunity in fish under As+NH3+T stress. Conversely, Toll-like receptor (TLR) expression was notably downregulated by Fe-NPs at 15 mg kg-1 diet in fish under As+NH3+T stress. Immunological attributes such as nitro blue tetrazolium chloride, total protein, albumin, globulin, A:G ratio, and myeloperoxidase (MPO) were improved by dietary Fe-NPs at 15 mg kg-1 diet in fish, regardless of stressors. The antioxidant genes (CAT, SOD, and GPx) were also strengthened by Fe-NPs in fish. Genes associated with growth performance, such as growth hormone regulator (GHR1 and GHRβ), growth hormone (GH), and insulin-like growth factor (IGF 1X and IGF 2X), were upregulated, enhancing fish growth under stress, while SMT and MYST were downregulated by Fe-NPs in the diet. Various growth performance indicators were improved by dietary Fe-NPs at 15 mg kg-1 diet. Notably, Fe-NPs also enhanced arsenic detoxification and reduced the cumulative mortality after a bacterial infection. In conclusion, this study highlights that dietary Fe-NPs can effectively mitigate arsenic and ammonia toxicity as well as high temperature stress by modulating gene expression in fish.

show abstract

Section: Discussionmentioning

confidence: 99%

Unraveling gene regulation mechanisms in fish: insights into multistress responses and mitigation through iron nanoparticles

Kumar,

Thorat,

Gunaware

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Similarly, no association with hsp genes was found to explain the genetic variability in the thermal tolerance landscape of DGRP lines. The proteins of the HSP family have a highly conserved sequence and structure in nature (Desai et al, 2010), and the DGRP population likely has low genetic variability in the genes encoding these proteins. Although these genes are important in the physiological response to thermal challenges, genetic variation could not be at the SNP level.…”

Section: Discussionmentioning

confidence: 99%

Genetic architecture of the thermal tolerance landscape ofDrosophila melanogaster

Soto,

Pinilla,

Olguín

et al. 2024

Preprint

View full text Add to dashboard Cite

Increased environmental temperatures associated with global warming strongly impact the natural populations of ectothermic species. Therefore, it is crucial to understand the genetic foundations and evolutionary potential of heat tolerance. However, heat tolerance and its genetic components depend on the methodology, making it difficult to predict the adaptive responses to global warming. Here, we measured the knockdown time for 100 lines from theDrosophilaGenetic Reference Panel (DGRP) at four different static temperatures, and we estimated their thermal death time (TDT) curves, which incorporate the magnitude and the time of exposure to thermal stress. Through quantitative genetic analyses, it was observed that the knockdown time showed a significant heritability at different temperatures and that its genetic correlations decreased as temperatures were dissimilar. Significant genotype-by-sex and genotype-by-environment interactions were noted for knockdown time. We also discovered heritable variation for the two parameters of TDT:CTmaxand thermal sensitivity. Taking advantage of the DGRP, we performed a GWAS and identified multiple variants positively associated with the TDT parameters, which map to genes related to signaling and developmental functions. We performed functional validations for some candidate genes using RNAi, which revealed that genes such asmam,KNCQ,orrobo3affect the knockdown time at a specific temperature but are not associated with the TDT parameters. Ultimately, the thermal tolerance landscape must possess the ability to adapt to the selective pressures caused by global warming. Genetic variation of phenotypic plasticity and sexually antagonistic pleiotropy should also facilitate the adaptive process and maintain the genetic diversity inDrosophilapopulations.

show abstract

Effects of a Mixture of Volatile Organic Compounds on Total DNA and Gene Expression of Heat Shock Proteins in Drosophila melanogaster

Doğanlar

2014

Arch Environ Contam Toxicol

View full text Add to dashboard Cite

The genotoxic effects of a mixture of 13 volatile organic compounds (VOCs) on total DNA profiles and the expression of heat shock proteins (HSPs) HSP26, HSP60, HSP70, and HSP83 in fruit fly tissues were examined. Drosophila melanogaster Oregon R(+), reared under controlled conditions on artificial diets, was treated with 13 VOCs commonly found in water at concentrations of 10, 20, 50, and 75 ppb for 1 and 5 days. Band changes were clearly detected in random amplified polymorphic DNA assay, especially at the 50- and 75-ppb exposure levels, for both treatment periods. In addition, there were clear differences in the band profiles of the treated and untreated flies with changes in the band intensity and the loss/appearance of bands. Although the genomic template stability (GTS) exhibited irregular changes at the first day, significant decreases in GTS were observed after 5 days of VOC application. The lowest GTS value (27.77 ± 1.96 %) was detected at the 75-ppb level after 5 days of the treatment. Quantitative real-time polymerase chain reaction analysis showed a significant increase in the relative expression of HSP26 and HSP60 after 1 and 5 days of the treatment, respectively. The expression of HSP70 increased significantly at all treatment concentrations and times. However, the greatest increase in expression level of HSP70 (4.2-fold) occurred at 20 ppb after 5 days of the treatment. HSP83 was the least affected by exposure to the VOCs. We conclude that trace levels of a mixture of VOCs can exert genotoxic effects on both total DNA and HSP levels in Drosophila.

show abstract

Hsp: Evolved and Conserved Proteins, Structure and Sequence Studies

Cited by 11 publications

References 26 publications

Unraveling gene regulation mechanisms in fish: insights into multistress responses and mitigation through iron nanoparticles

Unraveling gene regulation mechanisms in fish: insights into multistress responses and mitigation through iron nanoparticles

Genetic architecture of the thermal tolerance landscape ofDrosophila melanogaster

Effects of a Mixture of Volatile Organic Compounds on Total DNA and Gene Expression of Heat Shock Proteins in Drosophila melanogaster

Contact Info

Product

Resources

About