HSP25 overexpression attenuates oxidative stress–induced apoptosis: roles of ERK1/2 signaling and manganese superoxide dismutase

Lee, Yoonjin; Cho, Hae-Nyun; Jeoung, Dooil; Soh, Jae-Won; Cho, Chul Koo; Bae, Sangwoo; Chung, Hee Yong; Lee, Su‐Jae; Lee, Yun‐Sil

doi:10.1016/j.freeradbiomed.2003.11.009

Cited by 44 publications

(31 citation statements)

References 47 publications

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“…Upon heat shock, sHsps bind and stabilize cytoskeletal elements, exert antiapoptotic activity and modulate the oxidative and heat stress response, suggesting that sHsps can stabilize cytoskeletal proteins under both conditions (Concannon et al, 2003). There is also evidence for the role of ROS in directly regulating the activity of Hsps, in particular sHsps, through the oxidation of cysteine residues (Diaz-Latoud et al of sHsps has been shown to attenuate oxidative stress-induced apoptosis in mouse fibroblast cells (Lee et al, 2004). In summary, our data provide further support for the hypothesis that there are close links between cytoskeletal modifications caused directly by heat or through heat-induced oxidative stress and the increased expression of sHsps.…”

Section: Hypotheses Integrating the Observed Heat-stress-induced Chansupporting

confidence: 78%

The proteomic response of the mussel congenersMytilus galloprovincialisandM. trossulusto acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress

Tomanek

Zuzow

2010

Journal of Experimental Biology

272

273

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SUMMARYThe Mediterranean blue mussel, Mytilus galloprovincialis, an invasive species in California, has displaced the more heat-sensitive native congener, Mytilus trossulus, from its former southern range, possibly due to climate change. By comparing the response of their proteomes to acute heat stress we sought to identify responses common to both species as well as differences that account for greater heat tolerance in the invasive. Mussels were acclimated to 13°C for four weeks and exposed to acute heat stress (24°C, 28°C and 32°C) for 1h and returned to 13°C to recover for 24h. Using two-dimensional gel electrophoresis and tandem mass spectrometry we identified 47 and 61 distinct proteins that changed abundance in M. galloprovincialis and M. trossulus, respectively. The onset temperatures of greater abundance of some members of the heat shock protein (Hsp) 70 and small Hsp families were lower in M. trossulus. The abundance of proteasome subunits was lower in M. galloprovincialis but greater in M. trossulus in response to heat. Levels of several NADH-metabolizing proteins, possibly linked to the generation of reactive oxygen species (ROS), were lower at 32°C in the cold-adapted M. trossulus whereas proteins generating NADPH, important in ROS defense, were higher in both species. The abundance of oxidative stress proteins was lower at 32°C in M. trossulus only, indicating that its ability to combat heat-induced oxidative stress is limited to lower temperatures. Levels of NADdependent deacetylase (sirtuin 5), which are correlated with lifespan, were lower in M. trossulus in response to heat stress. In summary, the expression patterns of proteins involved in molecular chaperoning, proteolysis, energy metabolism, oxidative damage, cytoskeleton and deacetylation revealed a common loci of heat stress in both mussels but also showed a lower sensitivity to high-temperature damage in the warm-adapted M. galloprovincialis, which is consistent with its expanding range in warmer waters. Supplementary material available online at

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Section: Hypotheses Integrating the Observed Heat-stress-induced Chansupporting

confidence: 78%

The proteomic response of the mussel congenersMytilus galloprovincialisandM. trossulusto acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress

Tomanek

Zuzow

2010

Journal of Experimental Biology

272

273

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“…47 TNF-α activates the translocation of NF-κB and increases Mn-SOD. 48 However, Waon therapy did not modulate the cardiac expression of TNF-α and NF-κB signaling in TO-2 hamsters with heart failure (data not shown). We think that TNF-α and NF-κB have already increased in heart failure, therefore, the mechanisms by which Waon therapy increases Mn-SOD in failing hearts might not involve TNF-α/NF-κB pathway.…”

Section: Effect Of Waon Therapy On Oxidative Stress In Chfmentioning

confidence: 89%

Effect of Waon Therapy on Oxidative Stress in Chronic Heart Failure

Fujita

Ikeda

Miyata

et al. 2011

Circ J

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“…Homologs of Hsp24 belong to the alpha-crystallin family of small Hsps , and have been shown to be important chaperones in the stress responses of a diverse array of animals, including humans (Horwitz, 1992), brine shrimp Artemia franciscana (Clegg et al, 1999), the annual killifish Austrofundulus limnaeus (Podrabsky and Somero, 2004) and the mussel M. californianus (Gracey et al, 2008). Small Hsps have been shown to inhibit apoptosis (Beere, 2004) and to increase resistance to oxidative stress in mammalian cells (Lee et al, 2004). Mechanistically, Hsp24 is likely to act as a chaperone for maintaining cytoskeletal structural elements during stress (Derham and Harding, 1999), as well as preventing the aggregation of denatured proteins (Horwitz, 1992;Horwitz et al, 1992;Merck et al, 1993).…”

Section: Cytoskeletal Protection: a Potential Mechanism For Increasedmentioning

confidence: 99%

Transcriptomic responses to heat stress in invasive and native blue mussels (genus Mytilus): molecular correlates of invasive success

Lockwood

Sanders

Somero

2010

Journal of Experimental Biology

227

279

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SUMMARYInvasive species are increasingly prevalent in marine ecosystems worldwide. Although many studies have examined the ecological effects of invasives, little is known about the physiological mechanisms that might contribute to invasive success. The mussel Mytilus galloprovincialis, a native of the Mediterranean Sea, is a successful invader on the central and southern coasts of California, where it has largely displaced the native congener, Mytilus trossulus. It has been previously shown that thermal responses of several physiological traits may underlie the capacity of M. galloprovincialis to out-compete M. trossulus in warm habitats. To elucidate possible differences in stress-induced gene expression between these congeners, we developed an oligonucleotide microarray with 8874 probes representing 4488 different genes that recognized mRNAs of both species. In acute heat-stress experiments, 1531 of these genes showed temperature-dependent changes in expression that were highly similar in the two congeners. By contrast, 96 genes showed species-specific responses to heat stress, functionally characterized by their involvement in oxidative stress, proteolysis, energy metabolism, ion transport, cell signaling and cytoskeletal reorganization. The gene that showed the biggest difference between the species was the gene for the molecular chaperone small heat shock protein 24, which was highly induced in M. galloprovincialis and showed only a small change in M. trossulus. These different responses to acute heat stress may help to explain -and predict -the invasive success of M. galloprovincialis in a warming world. Supplementary material available online at

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HSP25 overexpression attenuates oxidative stress–induced apoptosis: roles of ERK1/2 signaling and manganese superoxide dismutase

Cited by 44 publications

References 47 publications

The proteomic response of the mussel congenersMytilus galloprovincialisandM. trossulusto acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress

The proteomic response of the mussel congenersMytilus galloprovincialisandM. trossulusto acute heat stress: implications for thermal tolerance limits and metabolic costs of thermal stress

Effect of Waon Therapy on Oxidative Stress in Chronic Heart Failure

Transcriptomic responses to heat stress in invasive and native blue mussels (genus Mytilus): molecular correlates of invasive success

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