Hsp60 expression profiles in the reef-building coral Seriatopora caliendrum subjected to heat and cold shock regimes

Seveso, Davide; Montano, Simone; Strona, Giovanni; Orlandi, Ivan; Galli, Paolo; Vai, Marina

doi:10.1016/j.marenvres.2016.05.007

Cited by 23 publications

(22 citation statements)

References 109 publications

(134 reference statements)

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“…A similar thermal exposure (33.5 • C for 6 days) of the zooxanthellate sea anemone Aiptasia also resulted in a reduced frequency of apoptosis but more frequent necrosis in both host and symbiont (Dunn et al, 2004). In the coral Seriatopora caliendrum, a collapse in the activity of heat shock protein 60 was seen in just 18 h after a sudden temperature increase to 33 • C (Seveso et al, 2016). This inhibition of the apoptotic protective mechanism, in conjunction with reduced antioxidation and cellular repair, indicates damage to, or overwhelming of, cellular acclimatory mechanisms and the rapid coral mortality of species with both high and low tolerance indicates a failure of protective mechanisms to safeguard the animal against heat-induced cell death.…”

Section: Coral Thermal Stress Responses and Mhwsmentioning

confidence: 87%

“…While both the coral host and its symbiont are physiologically challenged by the temperature stress associated with historical coral bleaching events (Baird et al, 2009), the symbiont has been considered as the more thermally sensitive partner in the symbiosis (Jones et al, 1998;Hoegh-Guldberg, 1999;Krug et al, 2013). This widely accepted understanding of coral bleaching has driven substantial research into determining the mechanisms underpinning damage to the endosymbiotic photosystems, a process generally described as photo-bleaching Leggat et al, 2011;Seveso et al, 2014Seveso et al, , 2016 Enzymatic antioxidants Increased production and activity of SOD, APX, CAT, GR, and DMSO (see Table 1) in order to counter the accumulation of oxygen radicals and prevent cellular damage Krueger et al, 2014Krueger et al, , 2015Roberty et al, 2015;Gardner et al, 2017 Non-enzymatic antioxidants Increased genetic expression of cytochrome P450 and heat shock proteins, with putative antioxidant properties Rosic et al, 2010 D1 repair and de novo synthesis (symbiont)…”

Section: Coral Thermal Stress Responses and Mhwsmentioning

confidence: 99%

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Marine Heatwave Hotspots in Coral Reef Environments: Physical Drivers, Ecophysiological Outcomes, and Impact Upon Structural Complexity

et al. 2019

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A changing climate is driving increasingly common and prolonged marine heatwaves (MHWs) and these extreme events have now been widely documented to severely impact marine ecosystems globally. However, MHWs have rarely been considered when examining temperature-induced degradation of coral reef ecosystems. Here we consider extreme, localized thermal anomalies, nested within broader increases in sea surface temperature, which fulfill the definitive criteria for MHWs. These acute and intense events, referred to here as MHW hotspots, are not always well represented in the current framework used to describe coral bleaching, but do have distinct ecological outcomes, including widespread bleaching, and rapid mass mortality of putatively thermally tolerant coral species. The physical drivers of these localized hotspots are discussed here, and in doing so we present a comprehensive theoretical framework that links the biological responses of the coral photo-endosymbiotic organism to extreme thermal stress and ecological changes on reefs as a consequence of MHW hotspots. We describe how the rapid onset of high temperatures drives immediate heat-stress induced cellular damage, overwhelming mechanisms that would otherwise mitigate the impact of gradually accumulated thermal stress. The warm environment, and increased light penetration of the coral skeleton due to the loss of coral tissues, coupled with coral tissue decay support rapid microbial growth in the skeletal microenvironment, resulting in the widely unrecognized consequence of rapid decay, and degeneration of the coral skeletons. This accelerated degeneration of coral skeletons on a reef scale hinder the recovery of coral populations and increase the likelihood of phase shifts toward algal dominance. We suggest that MHW hotspots, through driving rapid heatinduced mortality, compromise reefs' structural frameworks to the detriment of long term recovery. We propose that MHW hotspots be considered as a distinct class of thermal stress events in coral reefs, and that the current framework used to describe coral bleaching and mass mortality be expanded to include these. We urge further research into how coral mortality affects bioerosion by coral endoliths.

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Section: Coral Thermal Stress Responses and Mhwsmentioning

confidence: 87%

Section: Coral Thermal Stress Responses and Mhwsmentioning

confidence: 99%

Marine Heatwave Hotspots in Coral Reef Environments: Physical Drivers, Ecophysiological Outcomes, and Impact Upon Structural Complexity

et al. 2019

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“…One previous study on tropical reef‐building corals quantified levels of a thermal stress response protein in the same individuals during hot and cold stress, finding a strong increase in protein abundance under both treatments after 6 hr (Seveso et al., ). Such allied molecular responses are expected to reflect the ability of an organism to respond to multiple stressors using a common core stress response.…”

Section: Discussionmentioning

confidence: 99%

Extensive transcriptional variation poses a challenge to thermal stress biomarker development for endangered corals

et al. 2018

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As climate changes, sea surface temperature anomalies that negatively impact coral reef organisms continue to increase in frequency and intensity. Yet, despite widespread coral mortality, genetic diversity remains high even in those coral species listed as threatened. While this is good news in many ways, it presents a challenge for the development of biomarkers that can identify resilient or vulnerable genotypes. Taking advantage of three coral restoration nurseries in Florida that serve as long-term common garden experiments, we exposed over 30 genetically distinct Acropora cervicornis colonies to hot and cold temperature shocks seasonally and measured pooled gene expression responses using RNAseq. Targeting a subset of 20 genes, we designed a high-throughput qPCR array to quantify expression in all individuals separately under each treatment with the goal of identifying predictive and/or diagnostic thermal stress biomarkers. We observed extensive transcriptional variation in the population, suggesting abundant raw material is available for adaptation via natural selection. However, this high variation made it difficult to correlate gene expression changes with colony performance metrics such as growth, mortality and bleaching susceptibility. Nevertheless, we identified several promising diagnostic biomarkers for acute thermal stress that may improve coral restoration and climate change mitigation efforts in the future.

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“…One previous study on tropical reef-building corals quantified levels of a thermal stress response protein in the same individuals during hot and cold stress, finding a strong increase in protein abundance under both treatments after six hours (Seveso et al 2016). Such allied molecular responses are expected to reflect the ability of an organism to respond to multiple stressors using a common core stress response.…”

Section: Opposing Vs Allied Gene Expression Responsesmentioning

confidence: 97%

Extensive transcriptional variation poses a challenge to thermal stress biomarker development for endangered corals

Parkinson

Bartels

Devlin-Durante

et al. 2018

Preprint

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As climate changes, sea surface temperature anomalies that negatively impact coral reef organisms continue to increase in frequency and intensity. Yet, despite widespread coral mortality, genetic diversity remains high even in those coral species listed as threatened. While this is good news in many ways it presents a challenge for the development of biomarkers that can identify resilient or vulnerable genotypes. Taking advantage of three coral restoration nurseries in Florida that serve as long-term common garden experiments, we exposed over thirty genetically distinct Acropora cervicornis colonies to hot and cold temperature shocks seasonally and measured pooled gene expression responses using RNAseq. Targeting a subset of twenty genes, we designed a high-throughput qPCR array to quantify expression in all individuals separately under each treatment with the goal of identifying thermal stress biomarkers. We observed extensive transcriptional variation in the population, suggesting abundant raw material is available for adaptation via natural selection. However, this high variation made it difficult to correlate gene expression changes with colony performance metrics such as growth, mortality, and bleaching susceptibility. Nevertheless, we identified several promising biomarkers for acute thermal stress that may improve coral restoration and climate change mitigation efforts in the future.

show abstract

Hsp60 expression profiles in the reef-building coral Seriatopora caliendrum subjected to heat and cold shock regimes

Cited by 23 publications

References 109 publications

Marine Heatwave Hotspots in Coral Reef Environments: Physical Drivers, Ecophysiological Outcomes, and Impact Upon Structural Complexity

Marine Heatwave Hotspots in Coral Reef Environments: Physical Drivers, Ecophysiological Outcomes, and Impact Upon Structural Complexity

Extensive transcriptional variation poses a challenge to thermal stress biomarker development for endangered corals

Extensive transcriptional variation poses a challenge to thermal stress biomarker development for endangered corals

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