Hsp65-producing Lactococcus lactis inhibits experimental autoimmune encephalomyelitis by preventing cell migration into spinal cord

Guimarães, Mauro Andrade Freitas; Pinheiro-Rosa, Natália; Oliveira, Raquel Peres de; Aguiar, Sarah Leão Fiorini; Miranda, Mariana Camila Gonçalves; Lemos, Luísa; Souza, Adna L; Reis, Daniela Silva; Medeiros, Samara R.; Gonçalves, William; Amaral, Sylvia Stella; Pinho, Vanessa; Miyoshi, Anderson; Azevedo, Vasco A Z; Rezende, Rafael M.; Faria, Ana Maria Caetano

doi:10.1016/j.cellimm.2022.104661

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…In contrast, in PO3-treated cell lines, MBP, GFAP, and GLAC were significantly expressed at higher levels when compared with the MOG-treated cell lines. It has been reported that Hsp65-producing Lactococcus lactis inhibits experimental autoimmune encephalomyelitis by preventing cell migration into the spinal cord [35]. Furthermore, other probiotic species such as Bacillus amyloliquifaciens-supplemented camel milk suppress the neuroinflammation of autoimmune encephalomyelitis by regulating inflammatory markers [29], as well as by regulating SOX5/miR-218 axis orchestration in mouse models [30].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, this study aims to focus on the probiotic potential of Lactococcus raffinolactis and its role in the modulation of neuroinflammation. It has recently been reported that Lactococcus lactis displayed a significant positive impact in an LPS-induced depression-like model in mice [32], alleviated depressive and anxiety symptoms in mice [33], attenuated acute psychological stress in healthy Japanese men [34], and prevented the migration of cells into the spinal cord in experimental autoimmune encephalomyelitis [35]. Despite the above-mentioned beneficial effects of probiotic bacteria, little is known about the group of bacteria in cow milk in the Al-Ahsa region.…”

Section: Introductionmentioning

confidence: 99%

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Attenuation of Immunogenicity in MOG-Induced Oligodendrocytes by the Probiotic Bacterium Lactococcus Sp. PO3

Khalifa,

Ibrahim,

Sheikh

et al. 2023

Medicina

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Background and Objectives: Milk is healthy and includes several vital nutrients and microbiomes. Probiotics in milk and their derivatives modulate the immune system, fight inflammation, and protect against numerous diseases. The present study aimed to isolate novel bacterial species with probiotic potential for neuroinflammation. Materials and Methods: Six milk samples were collected from lactating dairy cows. Bacterial isolates were obtained using standard methods and were evaluated based on probiotic characteristics such as the catalase test, hemolysis, acid/bile tolerance, cell adhesion, and hydrophobicity, as well as in vitro screening. Results: Nine morphologically diverse bacterial isolates were found in six different types of cow’s milk. Among the isolates, PO3 displayed probiotic characteristics. PO3 was a Gram-positive rod cell that grew in an acidic (pH-2) salty medium containing bile salt and salinity (8% NaCl). PO3 also exhibited substantial hydrophobicity and cell adhesion. The sequencing comparison of the 16S rRNA genes revealed that PO3 was Lactococcus raffinolactis with a similarity score of 99.3%. Furthermore, PO3 was assessed for its neuroanti-inflammatory activity on human oligodendrocyte (HOG) cell lines using four different neuroimmune markers: signal transducer and activator of transcription (STAT-3), myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), and GLAC in HOG cell lines induced by MOG. Unlike the rest of the evaluated neuroimmune markers, STAT-3 levels were elevated in the MOG-treated HOG cell lines compared to the untreated ones. The expression level of STAT-3 was attenuated in both PO3-MOG-treated and only PO3-treated cell lines. On the contrary, in PO3-treated cell lines, MBP, GFAP, and GLAC were significantly expressed at higher levels when compared with the MOG-treated cell lines. Conclusions: The findings reported in this article are to be used as a foundation for further in vivo research in order to pave the way for the possible use of probiotics in the treatment of neuroinflammatory diseases, including multiple sclerosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Attenuation of Immunogenicity in MOG-Induced Oligodendrocytes by the Probiotic Bacterium Lactococcus Sp. PO3

Khalifa,

Ibrahim,

Sheikh

et al. 2023

Medicina

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“…In both regimens, everyday oral administration of the HSP-producing bacterial strain reduced the MS-associated clinical symptoms by activating the LAP + CD4 + Foxp3 + T cells in the spleen and the inguinal lymph nodes. Moreover, treated mice showed reduced leukocyte adherence in the venule structure of the spinal cord [ 70 ].…”

Section: Antigen Nonspecific Oral Immune Tolerancementioning

confidence: 99%

A Perspective on Oral Immunotherapeutic Tools and Strategies for Autoimmune Disorders

Shakya

Mallick²,

Nandakumar

2023

Vaccines

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Oral immune tolerance is a physiological process to achieve tolerance against autoimmunity by oral ingestion of self-antigen(s) or other therapeutics. At the cellular level, oral tolerance suppresses autoimmune diseases by activating FoxP-positive and -negative regulatory T cells (Tregs) and/or causing clonal anergy or deletion of autoreactive T cells, affecting B cell tolerance. However, oral delivery of antigens/biologics is challenging due to their instability in the harsh environment of the gastrointestinal (GI) tract. Several antigen/drug delivery tools and approaches, including micro/nanoparticles and transgenic plant-based delivery systems, have been explored to demonstrate oral immune tolerance for different autoimmune diseases successfully. However, despite the effectiveness, variation in results, dose optimization, and undesirable immune system activation are the limitations of the oral approach to further advancement. From this perspective, the current review discusses the oral tolerance phenomenon, cellular mechanisms, antigen delivery tools and strategies, and its challenges.

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Innovation and clinical progress in oral tolerance

Herzog

Weiner

Biswas

2023

Cellular Immunology

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Hsp65-producing Lactococcus lactis inhibits experimental autoimmune encephalomyelitis by preventing cell migration into spinal cord

Cited by 4 publications

References 41 publications

Attenuation of Immunogenicity in MOG-Induced Oligodendrocytes by the Probiotic Bacterium Lactococcus Sp. PO3

Attenuation of Immunogenicity in MOG-Induced Oligodendrocytes by the Probiotic Bacterium Lactococcus Sp. PO3

A Perspective on Oral Immunotherapeutic Tools and Strategies for Autoimmune Disorders

Innovation and clinical progress in oral tolerance

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