HSP70 as a marker of heat and humidity stress in Tarai buffalo

Manjari, Rao; Yadav, Mrigakshi; Ramesh, K.; Uniyal, Shweta; Rastogi, Sunil Kumar; Sejian, Veerasamy; Hyder, Iqbal

doi:10.1007/s11250-014-0692-4

Cited by 46 publications

(39 citation statements)

References 22 publications

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“…Buffaloes are more predisposed to heat stress than cattle (Manjari et al 2015). The cellular function in various tissues of the female reproductive tract, including the follicle, oocyte and embryo can be affected by hyperthermia (Hansen 2009).…”

Section: Discussionmentioning

confidence: 99%

Developmental competence and expression profile of genes in buffalo (Bubalus bubalis) oocytes and embryos collected under different environmental stress

et al. 2016

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The study examined the effects of different environmental stress on developmental competence and the relative abundance (RA) of various gene transcripts in oocytes and embryos of buffalo. Oocytes collected during cold period (CP) and hot period (HP) were matured, fertilized and cultured in vitro to blastocyst hatching stage. The mRNA expression patterns of genes implicated in developmental competence (OCT-4, IGF-2R and GDF-9), heat shock (HSP-70.1), oxidative stress (MnSOD), metabolism (GLUT-1), pro-apoptosis (BAX) and anti-apoptosis (BCL-2) were evaluated in immature and matured oocytes as well as in pre-implantation stage embryos. Oocytes reaching MII stage, cleavage rates, blastocyst yield and hatching rates increased (P \ 0.05) during the CP. In MII oocytes and 2-cell embryos, the RA of OCT-4, IGF-2R, GDF-9, MnSOD and GLUT-1 decreased (P \ 0.05) during the HP. In 4-cell embryos, the RA of OCT-4, IGF-2R and BCL-2 decreased (P \ 0.05) in the HP, whereas GDF-9 increased (P \ 0.05). In 8-to 16-cell embryos, the RA of OCT-4 and BCL-2 decreased (P \ 0. 05) in the HP, whereas HSP-70.1 and BAX expression increased (P \ 0.05). In morula and blastocyst, the RA of OCT-4, IGF-2R and MnSOD decreased (P \ 0.05) during the HP, whereas HSP-70.1 was increased (P \ 0.05). In conclusion, deleterious seasonal effects induced at the GVstage carry-over to subsequent embryonic developmental stages and compromise oocyte developmental competence and quality of developed blastocysts.

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Section: Discussionmentioning

confidence: 99%

Developmental competence and expression profile of genes in buffalo (Bubalus bubalis) oocytes and embryos collected under different environmental stress

et al. 2016

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“…Supporting the influence of seasonal changes as identified by Fader et al (1994) and Manjari et al (2015), Gaughan et al (2013) identified a photoperiod effect on HSP70 concentrations (r 2 = 0.94; P < 0.0001) in Angus feedlot steers. Indicating that the changes in day length, associated with the changing seasons, potentially influences the induction of the HSP response.…”

Section: Heat Shock Protein 70mentioning

confidence: 64%

“…Fader et al (1994) indicated that the induction of HSP70 in four fish species was incited by seasonal changes. Manjari et al (2015) also reported that mRNA expression of HSP70 in Tarai buffalo was twofold higher (P < 0.05) during the summer season. This indicates that the induction of the HSP response occurs at temperature thresholds to provide protection to protein structures during periods of changing climatic conditions.…”

Section: Heat Shock Protein 70mentioning

confidence: 84%

“…elevated body temperature, the rapid expression of HSP70 is critical for cytoprotection (Silver and Noble, 2012), highlighting HSP70 role in supporting homeostasis by maintaining cellular structures (Manjari et al, 2015) in response to climatic stressors. Fader et al (1994) indicated that the induction of HSP70 in four fish species was incited by seasonal changes.…”

Section: Heat Shock Protein 70mentioning

confidence: 99%

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Biological responses of feedlot cattle to heat load

Lees¹

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Heat load is a significant animal welfare and cost of production issue worldwide. In the US alone heat load is reported to have an annual economic burden of > $300 million in the beef sector.Furthermore animal growth is often depressed during summer resulting in heat related decreases in weight gain of approximately 10 kg which coincides with a 7 day increase in days on feed. The reduced growth rate increases days on feed, thereby increasing the cost of production. Whilst the effects of heat load on cattle has been researched for a number of years, there is speculation as to the interactions between hot environmental conditions and livestock performance, reproduction, health and overall wellbeing. Therefore there is a need to develop a more comprehensive understanding of the dynamic responses of animals to heat load. The key focus of heat load research is to develop effective management strategies to support animal comfort and performance during hot periods.While heat load can occur in pasture raised cattle, it is mostly observed within the intensive grain feed feedlot industry. Heat load occurs where a combination of environmental conditions exceed the animals ability to regulate body temperature, thus impacting on homeostasis. However how an animal responds to heat load is also dependent on a number of individual characteristics, including genotype, coat characteristics, health status and days on feed. Therefore no two animals will respond to hot climatic conditions in exactly the same manner. There are numerous responses to heat load that can be measured and/or observed in cattle, including changes in behaviour, respiratory dynamics, blood metabolites and body temperature. The experiments within this thesis were focused on investigating the; A key aspect in managing heat load is that feedlot personnel are able to recognise the responses of cattle to high heat load. Improvements in animal management have contributed to alleviating some of the negative effects of heat load. However summer conditions are still responsible for significant production losses and welfare concerns worldwide. Furthermore heat load cannot be completely eradicated where there are animal production operations in tropical and sub-tropical regions.Therefore the primary purpose of heat load research becomes focused on the effective use of mitigation strategies prior to and during heat related stress events, thus improving animal survivability and welfare during these events.iii Declaration by author Publications included in this thesisNo publications included. Contributions by others to the thesis

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“…55 Significantly higher expression values of HSP 70 was seen in buffalo during summer season (2.37±0.12) compared to winter (0.29±0.04). 56 The association between inducible HSP70.1 single nucleotide polymorphisms (SNPs) and the HS response of peripheral blood mononuclear cells, (PBMC) was studied in dairy cows by genotyping 446 Italian Holstein cows. 33 The presence of SNPs in the 5′-UTR region of inducible HSP70 showed amelioration of heat stress response and tolerance to heat stress in bovines.…”

Section: Types Of Hsps Studied In Livestock During Hsmentioning

confidence: 99%

Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

PR¹

2017

JDVAR

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The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during heat stress. The cellular and molecular responses in livestock are very crucial as it may lead to identification of confirmatory biomarker for heat stress in livestock. Thermo-tolerant gene expression and elevated heat shock protein (HSP) levels are observed to be the ultimate response through which the cell survives the heat stress. The HSPs have chaperonic activity ensuring the folding, unfolding and refolding of stress-denatured proteins. The components of heat shock response include heat shock factors (HSFs), heat shock element (HSE) and HSP. The cellular response to heat stress in mammalian organisms is controlled at the transcription level and it is mediated by a family of HSF which are regulated by the corresponding HSF genes. The activated HSFs bind with the HSE in the promoter region of HSP genes culminating in enhanced transcription of HSP mRNA. The HSP70, HSP90 and HSP27 are the predominant HSPs having protective role during heat stress in farm animals. Among these HSPs studied, HSP70 was identified to be the ideal biological marker for quantifying heat stress in animals.Keywords: heat stress, heat shock factors, heat shock response, hsp70, livestock Journal of Dairy, Veterinary & Animal Research Review Article Open AccessRole of heat shock proteins in livestock adaptation to heat stress the latter as it is harder to find an environment with a single variable changing. However, both these mechanisms are phenotypic responses as it is induced by the environment and the response goes once the stress is removed. It is usually produced to improve the fitness of the animal to the environment. Animals develop specific adaptive mechanisms if the environment stressors are present for a prolonged period. The mechanisms of adaptation include morphological, behavioural, biochemical and physiological changes. Biochemical adaptation to thermal stress involves changes in proteins, membrane lipids and metabolic rate.7 Genetic markers are of prior importance in the recent revolutionary developments in the field of molecular technology because of its viability as the biochemical adaptation can be utilized in livestock breeding programs compared to the behavioral, morphological or physiological responses to stress which is of less relevance. Heat shock protein is an important biomarker produced as cellular and tissue defense mechanism whose expression is markedly increased during heat shock. 8 The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during HS. General cellular responses to heat stressCellular exposure to thermal stress induces a number of anomalies in the functioning of cells which alters the biological molecules, disturbs cell functions, modulates metabolic reactions, induces oxidative cell damage and activates both apoptosis and necrosis pathways, ultimately leading to cell survival, acclimation or cell death depending on the time and...

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HSP70 as a marker of heat and humidity stress in Tarai buffalo

Cited by 46 publications

References 22 publications

Developmental competence and expression profile of genes in buffalo (Bubalus bubalis) oocytes and embryos collected under different environmental stress

Developmental competence and expression profile of genes in buffalo (Bubalus bubalis) oocytes and embryos collected under different environmental stress

Biological responses of feedlot cattle to heat load

Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

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