HSP70 Copurifies with Zika Virus Particles

Khachatoorian, Ronik; Cohn, Whitaker; Buzzanco, Anthony S.; Riahi, Rana; Arumugaswami, Vaithilingaraja; Dasgupta, Asim; Whitelegge, Julian P.; French, Samuel W.

doi:10.1016/j.virol.2018.07.009

Cited by 14 publications

(10 citation statements)

References 26 publications

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“…Evidence is growing that exposure of cells and tissues to low ambient temperature leads to transcription and translation of heat shock proteins 70 KDa (Hsp70) [9][10][11][12][13][14]. Moreover, Hsp70 has been shown to be one key factor for multiple virus infection including rabies virus [15], porcine circovirus type 2 [16], porcine reproductive and respiratory syndrome virus [17], influenza A virus [18], dengue virus [19], infectious bronchitis virus [20], Epstein-Barr virus [21], ectromelia virus [22], hepatitis B virus [23], enterovirus A71 [24] and zika virus [25,26]. Hsp70 plays important role in many steps of viral life cycle, including entry [27,28], uncoating [29], replication of the viral genome [30,31], gene expression [32] and virion morphogenesis [33,34].…”

Section: Introductionmentioning

confidence: 99%

Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro

Kong

Wei

et al. 2020

Pathogens

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Porcine epidemic diarrhea (PED) is a highly contagious, intestinal infectious disease caused by porcine epidemic diarrhea virus (PEDV). PEDV as an emerging and re-emerging epizootic virus of swine causes substantial economic losses to the pig industry in China and other countries. In China, the occurrence of PED shows significant seasonal variations, usually outbreak during the winter season. The epidemic characteristics of PED may be highly correlated with the changes of ambient temperature. However, molecular mechanism on the seasonal occurrence of PED still remains unclear. It has been widely observed that low ambient temperature up-regulates the expression of host heat shock protein 70 (Hsp70). Here, we showed that nucleotide and protein levels of Hsp70 were up-regulated in the intestinal of cold exposed pig and cold exposed Vero E6 cells. We found that overexpression of Hsp70 could increase PEDV mRNA synthesis and protein expression in Vero E6 and IPEC-J2 cells, while the siRNAs mediated knockdown of Hsp70 and VER155008 mediated inhibition of Hsp70 resulted in inhibition of viral mRNA synthesis and protein expression in Vero E6 cells. These data suggested that Hsp70 positively regulated PEDV mRNA synthesis and protein expression, which being helpful for understanding the seasonality of PED epidemics and development of novel antiviral therapies in the future.

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Section: Introductionmentioning

confidence: 99%

Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro

Kong

Wei

et al. 2020

Pathogens

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“…Neutralization assays revealed reduced viral infectivity when using an anti-annexin II antibody before DENV infection. Protein correlation profiling has been used to identify HSP70 in fractions co-migrating with ZIKV particles when separating intact protein complexes by density-gradient centrifugation and size-exclusion chromatography [ 182 ]. HSP70 is part of a family of conserved heat shock protein chaperones that are ubiquitously expressed on cells and have been shown to play a role in hepatitis C virus (HCV), DENV, and JEV infection [ 183–185 ].…”

Section: Mass Spectrometry-based Approaches To Study Flavivirus Infection Biologymentioning

confidence: 99%

Pathogenesis and virulence of flavivirus infections

et al. 2021

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The Flavivirus genus consists of >70 members including several that are considered significant human pathogens. Flaviviruses display a broad spectrum of diseases that can be roughly categorised into two phenotypes – systemic disease involving haemorrhage exemplified by dengue and yellow Fever virus, and neurological complications associated with the likes of West Nile and Zika viruses. Attempts to develop vaccines have been variably successful against some. Besides, mosquito-borne flaviviruses can be vertically transmitted in the arthropods, enabling long term persistence and the possibility of re-emergence. Therefore, developing strategies to combat disease is imperative even if vaccines become available. The cellular interactions of flaviviruses with their human hosts are key to establishing the viral lifecycle on the one hand, and activation of host immunity on the other. The latter should ideally eradicate infection, but often leads to immunopathological and neurological consequences. In this review, we use Dengue and Zika viruses to discuss what we have learned about the cellular and molecular determinants of the viral lifecycle and the accompanying immunopathology, while highlighting current knowledge gaps which need to be addressed in future studies.

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“…MS was also used to identify host proteins that interact with flaviviral particles. For example, Annexin II and HSP70 were found to associate with DENV and ZIKV particles, respectively, to enhance viral infection [30,31].…”

Section: Identifying the Interactomes Of Individual Viral Proteinsmentioning

confidence: 99%

Deciphering flavivirus–host interactions using quantitative proteomics

Ramage

Cherry

2020

Current Opinion in Immunology

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Flaviviruses are a group of important emerging and re-emerging human pathogens that cause worldwide epidemics with thousands of deaths annually. Flaviviruses are small, enveloped, positive-sense, single-stranded RNA viruses that are obligate intracellular pathogens, relying heavily on host cell machinery for productive replication. Proteomic approaches have become an increasingly powerful tool to investigate the mechanisms by which viruses interact with host proteins and manipulate cellular processes to promote infection. Here, we review recent advances in employing quantitative proteomics techniques to improve our understanding of the complex interplay between flaviviruses and host cells. We describe new findings on our understanding of how flaviviruses impact protein-protein interactions, protein-RNA interactions, protein abundance, and post-translational modifications to modulate viral infection.

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HSP70 Copurifies with Zika Virus Particles

Cited by 14 publications

References 26 publications

Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro

Cold Exposure-Induced Up-Regulation of Hsp70 Positively Regulates PEDV mRNA Synthesis and Protein Expression In Vitro

Pathogenesis and virulence of flavivirus infections

Deciphering flavivirus–host interactions using quantitative proteomics

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