Hsp70 Gene Family in Sebastiscus marmoratus: The Genome-Wide Identification and Transcriptome Analysis under Thermal Stress

Abstract

Heat shock protein 70 kDa (Hsp70) is a highly conserved heat stress protein that is important in biotic processes and responses to abiotic stress. Hsp70 genes may be important in Sebastiscus marmoratus, for it is a kind of nearshore reef fish, and habitat temperature change is more drastic during development. However, genome-wide identification and expression analysis in the Hsp70 gene family of S. marmoratus are still lacking. Here, a total of 15 Hsp70 genes in the genome of S. marmoratus are identified, and their expression patterns were investigated using transcriptomic data from thermal stress experiments. The expansion and gene duplication events of Hsp70 genes from the Hspa4, Hspa8, and Hspa12a subfamilies in S. marmoratus are revealed by phylogenetic analysis. qRT-PCR expression patterns demonstrated that seven Hsp70 genes were significantly up-regulated and none were significantly down-regulated after heat treatment. Only the hsp70 gene was significantly up-regulated after cold treatment. The selection test further showed a purifying selection on the duplicated gene pairs, suggesting that these genes underwent subfunctionalization. Our results add novel insight to aquaculture and biological research on S. marmoratus, providing important information on how Hsp70 genes are regulated in Scorpaeniformes under thermal stress.

Genome-Wide Identification and Transcriptome Analysis of the Hsp70 Gene Family in Monodonta labio Reveals Its Role in Response to Nanoplastics Stress

“…In the present work, local gene duplications are an important component of the mechanism hsp70 genes’ amplification in M. labio because all amplified hsc70 -like genes (9 copies) existed as tandem gene clusters. Various species have various numbers and kinds of duplicated genes, suggesting that these duplications may have separate origins [ 39 ]. Gene duplications and tandem duplications have been previously described in teleost fishes, echinoderms, and bivalve species as well as in crustaceans [ 39 , 40 ].…”

“…Various species have various numbers and kinds of duplicated genes, suggesting that these duplications may have separate origins [ 39 ]. Gene duplications and tandem duplications have been previously described in teleost fishes, echinoderms, and bivalve species as well as in crustaceans [ 39 , 40 ]. For example, previous studies indicated the expansion of the Hsp70 genes from Hspa4 (4 copies) in L. crocea , Hspa1 in B. pectinirostris (7 copies), Hspa12 in C. gigas ; P. yessoensi (73 copies and 57 copies), Hsc70 in P. trituberculatus and M. labio (4 and 9 copies, respectively).…”

“…hsc70l.3 and hsc70l.7 were significantly upregulated in the muscle after treatment with 0.1 and 1 mg/L NPs; however, hsc70l.5 was significantly downregulated after 10 mg/L NP stress treatment. Strong expression of hsc70l.3 and hsc70l.7 , at 0.1 and 1 mg/L, shielded cells from NP-induced misfolding and harm [ 39 ]. The production of Hsc70l.3 and Hsc70l.7 progressively increased, most likely due to the requirement for more Hsc70l.3 and Hsc70l.7 proteins, which encouraged aberrant proteins to renaturate [ 16 , 43 ].…”

Genome-Wide Identification, Molecular Characterization, and Expression Analysis of the HSP70 and HSP90 Gene Families in Thamnaconus septentrionalis

“…Additionally, except for zebrafish, the hsph1 gene is missing in most teleost genomes, implying a potential loss of the hsph1 gene during evolutionary processes. Similar to most teleosts [ 10 , 11 ], T. septentrionalis exhibits gene duplications within the hspa4 ( hspa4 , hspa4a , and hspa4l ) and hspa8 ( hspa8.1 and hspa8.2 ) genes. The HSP90 gene family members in T. septentrionalis showed no differences compared to other species [ 10 ], indicating a high degree of conservation of HSP90 genes in teleost evolution.…”

“…For example, in the Lateolabrax maculatus , investigations identified 5 HSP90 genes and 16 HSP70 genes, with their expression significantly upregulated in gills, liver, and muscle following thermal stress, suggesting their critical role in heat stress adaptation [ 10 ]. Similarly, in Sebastiscus marmoratus , 15 HSP70 genes were identified, with a majority showing upregulation under thermal stress [ 11 ]. Studies have also highlighted the differential expression of HSP70 genes in Boleophthalmus pectinirostris under ammonia stress [ 12 ], as well as the distinct expression patterns of HSP70 and HSP90 genes under osmotic and hypoxic stress [ 13 , 14 ].…”