Hsp90, Hsp60 and HSF-1 genes expression in muscle, heart and brain of thermally manipulated broiler chicken

Al-Zghoul, Mohammad-Borhan; Ismail, Zuhair Bani; Dalab, Abd Elhafeed S.; Al-Ramadan, Abdulla; Althnaian, Thnaian A.; Al-Ramadan, Saeed Y.; Ali, Abdelhadi M.; Albokhadaim, Ibrahim; Busadah, Khalid Ahmed Al; Eljarah, Abdulhakeem; Jawasreh, Khaleel; Hannon, Kevin

doi:10.1016/j.rvsc.2014.12.014

Cited by 45 publications

(30 citation statements)

References 36 publications

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“…Furthermore, to reduce metabolic heat production, peripheral T 3 is maintained low during high ambient temperature (Melesse et al 2011). Other reports have also described a significant drop in levels of T 3 and decreased metabolic rate in thermally conditioned chicken (Piestun et al 2011;Al-Zghoul et al 2015;Al-Rukibat et al 2017). The present observations differ from that of Rajkumar et al (2015a) where thermal manipulation did not alter the T 3 level chicken.…”

Section: Discussionmentioning

confidence: 77%

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Evaluation of DNA methylation and mRNA expression of heat shock proteins in thermal manipulated chicken

Vinoth

Thirunalasundari

Shanmugam

et al. 2018

Cell Stress and Chaperones

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Thermal manipulation during embryogenesis has been demonstrated to enhance the thermotolerance capacity of broilers through epigenetic modifications. Heat shock proteins (HSPs) are induced in response to stress for guarding cells against damage. The present study investigates the effect of thermal conditioning during embryogenesis and thermal challenge at 42 days of age on HSP gene and protein expression, DNA methylation and in vitro luciferase assay in brain tissue of Naked Neck (NN) and Punjab Broiler-2 (PB-2) chicken. On the 15th day of incubation, fertile eggs from two breeds, NN and PB-2, were randomly divided in to two groups: control (C)-eggs were incubated under standard incubation conditions, and thermal conditioning (TC)-eggs were exposed to higher incubation temperature (40.5°C) for 3 h on the 15th, 16th, and 17th days of incubation. The chicks obtained from each group were further subdivided and reared under different environmental conditions from the 15th to the 42nd day as normal [N; 25 ± 1 °C, 70% relative humidity (RH)] and heat exposed (HE; 35 ± 1 °C, 50% RH) resulting in four treatment groups (CN, CHE, TCN, and TCHE). The results revealed that HSP promoter activity was stronger in CHE, which had lesser methylation and higher gene expression. The activity of promoter region was lesser in TCHE birds that were thermally manipulated at the embryonic stage, thus reflecting their stress-free condition. This was confirmed by the lower level of mRNA expression of all the HSP genes. In conclusion, thermal conditioning during embryogenesis has a positive impact and improves chicken thermotolerance capacity in postnatal life.

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Section: Discussionmentioning

confidence: 77%

“…The higher increase in corticosterone levels in heat-exposed groups in the present study is similar to other reports (Piestun et al 2008;Melesse et al 2011). Al-Zghoul et al (2015) had reported no change in corticosterone concentration after thermal challenge in birds that underwent thermal manipulation during the embryonic age.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of DNA methylation and mRNA expression of heat shock proteins in thermal manipulated chicken

Vinoth

Thirunalasundari

Shanmugam

et al. 2018

Cell Stress and Chaperones

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“…The differences between HSP70 mRNA and protein levels in response to heat stress may be a result of multifaceted regulation of transcription and translation rate as well as mRNA and/or protein accumulation and degradation in the cell (Schwanh€ ausser et al 2011). Other studies have also shown that different heat stress manipulations modulate expression of HSP genes in the chick heart, liver, muscle, and brain, implying that relative mRNA and/or protein level of HSP proteins may use as a marker for heat stress (Yahav et al 1997;Wang and Edens 1998;Xie et al 2014;Al-Zghoul et al 2015;Sun et al 2015). However, the long-term regulatory mechanism underlying differential HSP gene expression in response to heat stress was not described previously.…”

Section: Discussionmentioning

confidence: 91%

Methyl CpG level at distal part of heat‐shock protein promoter HSP70 exhibits epigenetic memory for heat stress by modulating recruitment of POU2F1‐associated nucleosome‐remodeling deacetylase (NuRD) complex

Kisliouk

Cramer

Meiri

2017

Journal of Neurochemistry

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Depending on its stringency, exposure to heat in early life leads to either resilience or vulnerability to heat stress later in life. We hypothesized that epigenetic alterations in genes belonging to the cell proteostasis pathways are attributed to long-term responses to heat stress. Epigenetic regulation of the mRNA expression of the molecular chaperone heat-shock protein (HSP) 70 (HSPA2) was evaluated in the chick hypothalamus during the critical period of thermal-control establishment on day 3 post-hatch and during heat challenge on day 10. Both the level and duration of HSP70 expression during heat challenge a week after heat conditioning were more pronounced in chicks conditioned under harsh versus mild temperature. Analyzing different segments of the promoter in vitro indicated that methylation of a distal part altered its transcriptional activity. In parallel, DNA-methylation level of this segment in vivo was higher in harsh- compared to mild-heat-conditioned chicks. Hypermethylation of the HSP70 promoter in high-temperature-conditioned chicks was accompanied by a reduction in both POU Class 2 Homeobox 1 (POU2F1) binding and recruitment of the nucleosome remodeling deacetylase (NuRD) chromatin-remodeling complex. As a result, histone H3 acetylation levels at the HSP70 promoter were higher in harsh-temperature-conditioned chicks than in their mild-heat-conditioned counterparts. These results suggest that methylation level of a distal part of the HSP70 promoter and POU2F1 recruitment may reflect heat-stress-related epigenetic memory and may be useful in differentiating between individuals that are resilient or vulnerable to stress.

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“…Thermal manipulation ( TM ) during broiler embryogenesis, by increasing or decreasing the incubation temperature, was suggested to enhance heat tolerance during posthatch life ( Piestun et al., 2008 , Molenaar et al., 2010 , Al-Zghoul et al., 2013 , Al-Zghoul et al., 2015b , Al-Rukibat et al., 2017 ). Heat-tolerance improvement was achieved by the modulation of thyroid hormones, which are major players in the basal metabolic rate regulation, and HSP levels ( Al-Zghoul et al., 2013 , Al-Zghoul et al., 2015a , Al-Zghoul et al., 2015b , Al-Zghoul et al., 2015c , Al-Rukibat et al., 2017 ). Previously, TM was reported to increase Hsp70, HSF3, IL-1β, TNF-α, IL-6, TLR2, and TLR4 and to decrease catalase, GPX, SOD, and NOX expression in broiler chickens during acute heat stress ( Al-Zghoul et al., 2018 , Al-Zghoul et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of thermal manipulation of eggs on the response of jejunal mucosae to posthatch chronic heat stress in broiler chickens

Al‐Zghoul

Saleh

2020

Poultry Science

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In this study, the aim was to investigate effects of chronic heat stress ( CHS ) on the mRNA levels of proinflammatory cytokines (interleukin [ IL ]-6, IL-8, IL-1β, and tumor necrosis factor alpha [ TNF-α ]), toll-like receptors (TLR2 and TLR4), heat shock proteins (Hsp70, heat shock transcription factor [ HSF ]-1, and HSF3) and antioxidant enzymes (catalase, glutathione peroxidase, NADPH oxidase, and superoxide-dismutase) in the jejunal mucosae of broiler chickens subjected to thermal manipulation ( TM ) during embryogenesis. TM was carried out at 39°C and 65% relative humidity ( RH ) for 18 h daily from embryonic days 10 to 18. Control group was incubated at 37.8°C and 56% RH. CHS was induced by raising the temperature to 35°C for 7 D throughout posthatch days 28 to 35. On post-hatch-day 28 (day zero of CHS) and after 1, 3, 5, and 7 D of CHS, the jejunal mucosae were collected from both groups to evaluate the mRNA levels by real-time reverse transcription-PCR analysis. On day zero of CHS, the mRNA levels of antioxidant enzymes, TLRs, HSF3, IL-1β, and TNF-α were not significantly different between TM and control groups, while the levels of IL-6, IL-8, and HSF1 were lower and the level of Hsp70 was higher in TM. However, during CHS, the mRNA levels of antioxidant enzymes, IL-1β, TNF-α, TLR4, and HSF1 were significantly lower in TM than in controls, while the levels of TLR2 and IL-8 were significantly higher in TM than in controls. In addition, TM led to significant increase of mRNA levels of IL-6 and HSF3 after 1 D and Hsp70 after 3 D of CHS and to significant decrease of mRNA levels of IL-6 after 3 and 5 D, HSF3 after 7 D, and Hsp70 after 5 D of CHS. Results of this study suggest that TM led to altered posthatch antioxidant, immunological, and Hsp response to CHS in the jejunal mucosae of broiler chickens, probably indicating that TM may mitigate the adverse effects of CHS.

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Hsp90, Hsp60 and HSF-1 genes expression in muscle, heart and brain of thermally manipulated broiler chicken

Cited by 45 publications

References 36 publications

Evaluation of DNA methylation and mRNA expression of heat shock proteins in thermal manipulated chicken

Evaluation of DNA methylation and mRNA expression of heat shock proteins in thermal manipulated chicken

Methyl CpG level at distal part of heat‐shock protein promoter HSP70 exhibits epigenetic memory for heat stress by modulating recruitment of POU2F1‐associated nucleosome‐remodeling deacetylase (NuRD) complex

Effects of thermal manipulation of eggs on the response of jejunal mucosae to posthatch chronic heat stress in broiler chickens

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