HSPA6 and its role in cancers and other diseases

Song, Binghui; Shen, Shiyi; Fu, Shangyi; Fu, Junjiang

doi:10.1007/s11033-022-07641-5

Cited by 22 publications

(17 citation statements)

References 98 publications

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“…The most enriched CC terms were nucleosome and extracellular matrix. The most enriched MF terms were protein heterodimerization activity and potassium channel activity ◂ family A (HSP70) member 6 (HSPA6) encode 70 kDa heat shock proteins that belong to the HSP70 family [25,26]. These results suggest that HSPs may play an important role in 5-ALA-PDT and remain to be further mechanistically explored.…”

Section: Validation Of the Sequencing Results By Rt-qpcr And Western ...mentioning

confidence: 99%

RNA-Seq-based transcriptomics analysis during the photodynamic therapy of primary cells in secondary hyperparathyroidism

Wen

Zeng

Chen

et al. 2023

Photochem Photobiol Sci

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Background The aim of this study was to identify changes in gene expression before and after 5-aminolevulinic acid-mediated photodynamic therapy (5-ALA-PDT) and to investigate the potential mechanism of 5-ALA-PDT based on ribonucleic acid sequencing (RNA-Seq) analysis. Methods Secondary hyperparathyroidism (SHPT) primary cells were isolated from surgically excised specimens and exposed to laser light. The transcription profiles of SHPT primary cells were identified through RNA-Seq. Differentially expressed genes (DEGs) were identified. Enrichment of functions and signaling pathway analysis were performed based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Quantitative real-time polymerase chain reaction (RT-qPCR) and western blot analysis were used to validate genes based on RNA-Seq results. Results In total, 1320 DEGs were identified, of which 1019 genes were upregulated and 301 genes were downregulated. GO and KEGG pathway analyses identified significantly enriched pathways in DEGs, including TGF beta in extracellular matrix (ECM), negative regulation of triglyceride biosynthetic process, protein heterodimerization activity, systemic lupus erythematosus, ECM-receptor interaction, focal adhesion and protein digestion and absorption. Protein-protein interaction (PPI) network analyses identified potential heat shock protein (HSP) interactions among the DEGs. Eight HSP genes were also identified that were most likely involved in 5-ALA-PDT, which were further validated by RT-qPCR and western blotting.

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Section: Validation Of the Sequencing Results By Rt-qpcr And Western ...mentioning

confidence: 99%

RNA-Seq-based transcriptomics analysis during the photodynamic therapy of primary cells in secondary hyperparathyroidism

Wen

Zeng

Chen

et al. 2023

Photochem Photobiol Sci

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“…HSPA6 belongs to the HSP70 family and appears evolutionary unique as it is not conserved in rodents. HSPA6 expression is not detectable in most cells under normal conditions 10 . Recent studies have found that HSPA6 exhibits to inhibit tumorigenesis and tumor progression in some types of cancers but promotes it in others.…”

Section: Discussionmentioning

confidence: 98%

“…HSPA6 expression is not detectable in most cells under normal conditions. 10 Recent studies have found that HSPA6 exhibits to inhibit tumorigenesis and tumor progression in some types of cancers but promotes it in others. However, HSPA6 expression and its exact roles in GC are still unclear.…”

Section: Discussionmentioning

confidence: 99%

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HSPA6, a novel prognostic and therapeutic biomarker, associated with Ming classification in gastric cancer

Zhang

Zhuo

Hong

et al. 2022

Clinical Laboratory Analysis

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“…We discovered NAC treatment reduced mRNA and protein expressions of HSPA6. HSPA6 is a strictly stress-inducible member of Hsp70 family and has low or nondetectable expression levels in most cells [ 27 ]. However, it has been found to be expressed in response to cellular stresses including viral infection, such as tick-borne encephalitis virus [ 28 ] and Enterovirus 71 [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

N-Acetyl-L-Cysteine Protects Airway Epithelial Cells during Respiratory Syncytial Virus Infection against Mucin Synthesis, Oxidative Stress, and Inflammatory Response and Inhibits HSPA6 Expression

Chi¹,

Shan²,

Cui³

2022

Analytical Cellular Pathology

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Objective. Respiratory syncytial virus (RSV) infection is an important cause of hospitalization of children worldwide, leading to significant morbidity and mortality. RSV infection leads to increasing inflammatory and apoptosis events in the airway epithelium through mechanisms involving ROS generation. The antioxidant N-acetyl-L-cysteine (NAC) has been shown to inhibit influenza virus replication and to reduce the secretion of inflammatory and apoptotic mediators during virus infection. The study aims to investigate the effects of NAC on human bronchial epithelial cells BEAS-2B and HSPA6 expression during RSV infection. Methods. CCK-8 assays were performed to evaluate cell survival. The production of proinflammatory factors, TNF-α, IL-6, IL-1β, IL-18, and MUC5AC was examined by quantitative real-time PCR and ELISA. Oxidative stress was determined by reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH)/glutathione disulfide (GSSG) ratio. Immunoblotting analysis of epidermal growth factor receptor (EGFR) and its phosphorylation was performed. The antiviral effect of NAC was assessed by determining viral titers using plaque assay. Results. RSV infection reduced cell survival, promoted the release of proinflammatory factors, increased the ROS production and MDA concentration, and diminished the SOD activity and GSH/GSSG ratio, all which were attenuated by NAC treatment. Accordingly, NAC treatment inhibited the activation of EGFR and MUC5AC in BEAS-2B cells with RSV infection. Furthermore, NAC administration resulted in a marked decrease in RSV-induced HSPA6 expression in BEAS-2B cells. Concomitantly, EPB treatment led to an evident inhibition of RSV fusion gene and viral replication in RSV-infected BEAS-2B cells. Conclusion. This work supports the use of NAC to exert antimucin synthesis, anti-inflammatory, antioxidant, and antiviral effects on airway epithelium during RSV infection.

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HSPA6 and its role in cancers and other diseases

Cited by 22 publications

References 98 publications

RNA-Seq-based transcriptomics analysis during the photodynamic therapy of primary cells in secondary hyperparathyroidism

RNA-Seq-based transcriptomics analysis during the photodynamic therapy of primary cells in secondary hyperparathyroidism

HSPA6, a novel prognostic and therapeutic biomarker, associated with Ming classification in gastric cancer

N-Acetyl-L-Cysteine Protects Airway Epithelial Cells during Respiratory Syncytial Virus Infection against Mucin Synthesis, Oxidative Stress, and Inflammatory Response and Inhibits HSPA6 Expression

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