HspB1 Overexpression Improves Life Span and Stress Resistance in an Invertebrate Model

Alexander, Courtney; Munkáscy, Erin; Tillmon, Haven; Fraker, Tamara; Scheirer, Jessica; Holstein, Deborah; Lozano, Damian; Khan, Maruf; Gidalevitz, Tali; Lechleiter, James D.; Fisher, Alfred L.; Zare, Habil; Rodriguez, Karl A.

doi:10.1093/gerona/glab296

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…HSPB1 is a member of the heat shock protein molecular family and is an ATP-independent protein molecular chaperone [31] . It is mainly involved in the maintenance of cell homeostasis, inhibition of cell aging, and antioxidant stress.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of co-disease genes between COVID-19 and osteoarthritis

Zhang,

Liu,

et al. 2024

Preprint

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Background: The molecular mechanisms of corona virus disease 2019 (COVID-19) and osteoarthritis (OA) are unclear, and there is an urgent need to identify new biomarkers and explore their potential molecular mechanisms in COVID-19 and OA. Methods: The GSE57218, GSE157103 training sets and the GSE82107, GSE171110 validation sets were acquired via gene expression omnibus (GEO) database. First, differentially expressed genes (DEGs) between disease and normal samples in the GSE57218 and GSE157103 training sets were respectively sifted out by differential expression analysis. The modules with the highest correlation with OA and normal, COVID-19 and non COVID-19 were gained by weighted gene co-expression network analysis (WGCNA), individually. Then, OA-DEGs were intersected with the module genes that had significant correlation with OA, and COVID-19-DEGs were intersected with the module genes which were dramatically correlated with COVID-19 to yield OA-intersected genes and COVID-19 intersected genes, respectively. The OA-intersected genes and COVID-19 intersected genes were intersected to yield candidate genes, and they were analyzed for function enrichment analysis. Next, the seven algorithms (Closeness, MCC, Degree, MNC, Radality, Stress and EPC) were performed on candidate genes to sift out biomarkers. Finally, we constructed the competing endogenous RNA (ceRNA), transcription factor (TF)/miRNA-mRNA and drug-target regulatory networks. Results:There were 1135 OA-DEGs and 4336 COVID-19-DEGs between disease and normal samples in the GSE57218 and GSE157103 training sets, respectively. The pink, blue and brown modules had significant correlations with OA in the GSE57218 training set, while in the GSE157103 training set, the pink and brown modules were notably correlated with COVID-19. We finally yield 715 OA-intersected genes and 2282 COVID-19-intersected genes. After intersecting the above two intersected genes, we gained 106 candidate genes, and they were involved in ADP metabolic process, nucleoside diphosphate phosphorylation, etc.. The 7 biomarkers, namely AK1, APP, ENO1, TPI1, HSP90B1, HSPB1 and ESR1, were acquired based on seven algorithms. Finally, we successfully constructed the ceRNA, TF/miRNA-mRNA and drug-target networks. Conclusion: Through bioinformatic methods, we explored the biomarkers (AK1, APP, ENO1, TPI1, HSP90B1, HSPB1 and ESR1) of COVID-19 combined OA, providing new ideas for studies related to molecular mechanisms and treatment of comorbidity.

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Section: Discussionmentioning

confidence: 99%

Identification and analysis of co-disease genes between COVID-19 and osteoarthritis

Zhang,

Liu,

et al. 2024

Preprint

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“…In this study, we identified PHF20 as a candidate gene that was differentiated between LB and BR and we suggest further studies to investigate its role in the chicken osteoblast differentiation. In addition, candidate genes such as mitogen‐activated protein kinase 3 (MAPK3), which is involved in follicle development in chickens (Zhang et al, 2022 ), or the expression of HspB1, which prolongs lifespan and increases resistance to heat stress (Alexander et al, 2022 ), have also been identified as functional candidate genes that differ between LB and BR.…”

Section: Discussionmentioning

confidence: 99%

RNA‐Seq‐based discovery of genetic variants and allele‐specific expression of two layer lines and broiler chicken

Iqbal

Hadlich

Reyer

et al. 2023

Evolutionary Applications

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Recent advances in the selective breeding of broilers and layers have made poultry production one of the fastest‐growing industries. In this study, a transcriptome variant calling approach from RNA‐seq data was used to determine population diversity between broilers and layers. In total, 200 individuals were analyzed from three different chicken populations (Lohmann Brown (LB), n = 90), Lohmann Selected Leghorn (LSL, n = 89), and Broiler (BR, n = 21). The raw RNA‐sequencing reads were pre‐processed, quality control checked, mapped to the reference genome, and made compatible with Genome Analysis ToolKit for variant detection. Subsequently, pairwise fixation index (FST) analysis was performed between broilers and layers. Numerous candidate genes were identified, that were associated with growth, development, metabolism, immunity, and other economically significant traits. Finally, allele‐specific expression (ASE) analysis was performed in the gut mucosa of LB and LSL strains at 10, 16, 24, 30, and 60 weeks of age. At different ages, the two‐layer strains showed significantly different allele‐specific expressions in the gut mucosa, and changes in allelic imbalance were observed across the entire lifespan. Most ASE genes are involved in energy metabolism, including sirtuin signaling pathways, oxidative phosphorylation, and mitochondrial dysfunction. A high number of ASE genes were found during the peak of laying, which were particularly enriched in cholesterol biosynthesis. These findings indicate that genetic architecture as well as biological processes driving particular demands relate to metabolic and nutritional requirements during the laying period shape allelic heterogeneity. These processes are considerably affected by breeding and management, whereby elucidating allele‐specific gene regulation is an essential step towards deciphering the genotype to phenotype map or functional diversity between the chicken populations. Additionally, we observed that several genes showing significant allelic imbalance also colocalized with the top 1% of genes identified by the FST approach, suggesting a fixation of genes in cis‐regulatory elements.

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“…12 In the present study, we have shown a significant improvement in life span in nonylphenol-exposed Np1-Gal4 > UAS-Hsp27 flies compared with Np1-Gal4> w 1118 flies. Alexander et al 31 showed that Hsp27 (HspB1) improves the life span of the organisms by decreasing the oxidative stress in exposed Caenorhabditis elegans.…”

Section: Discussionmentioning

confidence: 99%

Protective Role of Hsp27 in the Nonylphenol-Induced Locomotory and Longevity Toxicity

Dwivedi

Francis

Sharma

2023

Journal of Health and Allied Sciences NU

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Background Gut health is directly proportional to an organism's fitness. Our recent study showed a functional link between oxidative stress and heat shock protein 27 (Hsp27, a stress protein) in the Drosophila larval gut, which coordinates the nonylphenol (an endocrine disruptor) allied sub-cellular and developmental adversities. Objective In continuation with the prior study, the present study aimed to explore the association of Hsp27 with locomotory and survival against nonylphenol-induced toxicity in the Drosophila gut. Methods and Methodology The freshly emerged adult flies were exposed to nonylphenol (5.0 µg/mL) for 10 to 40 days, and their locomotory performance (climbing activity) and survivability were assessed. ANOVA was used to evaluate the statistical significance of the mean values in control and treated flies. Results Nonylphenol exposure markedly influenced locomotory activity and survivability after 30 to 40 days. For instance, ∼76% (40 days) declined locomotor behavior, and ∼35% (40 days) reduced survivability was observed. While the overexpression of Hsp27 in the organism's gut showed improvement in locomotory performance and survivability after 30 to 40 days. No significant alteration in locomotory performance and survivability was observed after 10 to 20 days of nonylphenol exposure. Conclusion The present study illustrates that Hsp27 overexpression in the Drosophila gut improves the locomotory performance and survivability in the nonylphenol exposed Drosophila. This also indicates the possible connection between the gut and organismal fitness.

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HspB1 Overexpression Improves Life Span and Stress Resistance in an Invertebrate Model

Cited by 13 publications

References 40 publications

Identification and analysis of co-disease genes between COVID-19 and osteoarthritis

Identification and analysis of co-disease genes between COVID-19 and osteoarthritis

RNA‐Seq‐based discovery of genetic variants and allele‐specific expression of two layer lines and broiler chicken

Protective Role of Hsp27 in the Nonylphenol-Induced Locomotory and Longevity Toxicity

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