HT‐SuperSAGE of the gut tissue of a Vip3Aa‐resistant <i>Heliothis virescens</i> (Lepidoptera: Noctuidae) strain provides insights into the basis of resistance

Ayra‐Pardo, Camilo; Ochagavía, María Elena; Raymond, Ben; Gulzar, Asim; Rodríguez-Cabrera, Lianet; Noval, C.; Bertot, Ivis Morán; Terauchi, Ryohei; Yoshida, Kentaro; Matsumura, Hideo; Rodríguez, Pilar Téllez; Hernández, Daily; Borrás‐Hidalgo, Orlando; Wright, Denis J.

doi:10.1111/1744-7917.12535

Cited by 8 publications

(6 citation statements)

References 81 publications

(131 reference statements)

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“…Even though differences in binding were not found, a dramatic reduction in the ALP enzymatic activity was detected in midgut samples from the resistant compared to susceptible colony. Western blotting and RT-qPCR analyses showed that the decreased activity was due to a reduction in the amount of mALP protein, which was controlled at the transcriptional level, in agreement with a previous study [ 20 ]. Downregulation or reduced levels of mALP in the midgut membrane have been observed as a common phenomenon in resistance to Cry1Ac in H. virescens [ 25 ], Helicoverpa zea [ 21 ], Plutella xylostella [ 22 ], and Helicoverpa armigera [ 24 ]; to Cry1F in S. frugiperda [ 23 ]; to Cry1C in Spodoptera litura [ 34 ]; and even in Aedes aegypti resistant to Bt subsp.…”

Section: Discussionsupporting

confidence: 92%

“…In a previous study with this colony, resistance was shown to be polygenic, conferring little cross-resistance to Cry1Ab and no cross-resistance to Cry1Ac [ 19 ]. A transcriptomic analysis detected significant differences in gene expression compared to a susceptible strain, with 420 over-expressed and 1569 under-expressed genes in Vip-Sel [ 20 ]. Results herein support that Vip3Aa binding is not significantly altered in Vip-Sel compared to susceptible H. virescens and that membrane bound alkaline phosphatase (mALP) is not involved in Vip3Aa binding.…”

Section: Introductionmentioning

confidence: 99%

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Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony

Pinos

Chakroun

Millán-Leiva

et al. 2020

Toxins

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The Vip3Aa insecticidal protein from Bacillus thuringiensis (Bt) is produced by specific transgenic corn and cotton varieties for efficient control of target lepidopteran pests. The main threat to this technology is the evolution of resistance in targeted insect pests and understanding the mechanistic basis of resistance is crucial to deploy the most appropriate strategies for resistance management. In this work, we tested whether alteration of membrane receptors in the insect midgut might explain the >2000-fold Vip3Aa resistance phenotype in a laboratory-selected colony of Heliothis virescens (Vip-Sel). Binding of 125I-labeled Vip3Aa to brush border membrane vesicles (BBMV) from 3rd instar larvae from Vip-Sel was not significantly different from binding in the reference susceptible colony. Interestingly, BBMV from Vip-Sel larvae showed dramatically reduced levels of membrane-bound alkaline phosphatase (mALP) activity, which was further confirmed by a strong downregulation of the membrane-bound alkaline phosphatase 1 (HvmALP1) gene. However, the involvement of HvmALP1 as a receptor for the Vip3Aa protein was not supported by results from ligand blotting and viability assays with insect cells expressing HvmALP1.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony

Pinos

Chakroun

Millán-Leiva

et al. 2020

Toxins

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“…RNAi-mediated silencing of RpS2 gene expression in both transfected Sf21 cells and in larvae of Spodoptera litura (Fabricius, 1775) injected with double-stranded RNA resulted in reduced toxicity of the Vip3A protein. Further evidence for the specific upregulation of ribosomal proteins in response to a selective agent was provided by HT-SuperSAGE analysis of a Vip3Aa-selected population of Heliothis virescens (Fabricius, 1777), which confirmed this phenomenon [58]. Whether the constitutive overexpression of ribosomal proteins in acetamiprid-selected pea aphids responds to an increased demand for protein synthesis in response to stress or is involved in a specific adaptive mechanism remains to be elucidated.…”

Section: Discussionmentioning

confidence: 90%

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid <em>Acyrthosiphon pisum</em> (Hemiptera: Aphididae) to Acetamiprid

Cai,

Zhao,

Qian

et al. 2024

Preprint

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Acetamiprid is a broad-spectrum neonicotinoid insecticide used in agriculture to control aphids. While recent studies have documented resistance to acetamiprid in several aphid species, the underlying mechanisms are still not fully understood. In this study, we analyzed the transcriptome and metatranscriptome of a laboratory strain of the pea aphid, Acyrthosiphon pisum (Harris, 1776), with reduced susceptibility to acetamiprid after nine generations of exposure to identify candidate genes and the microbiome involved in the adaptation process. Sequencing of the transcriptome of both selected (RS) and non-selected (SS) strains allowed the identification of 14858 genes and 4938 new transcripts. Most of the differentially expressed genes were associated with catalytic activities and metabolic pathways involving carbon and fatty acids. Specifically, the alcohol-forming fatty acyl-CoA reductase (FAR) and acyl-CoA synthetase (ACSF2), both involved in the synthesis of epidermal wax layer components, were significantly upregulated in RS, suggesting that adaptation to acetamiprid involves the synthesis of a thicker protective layer. Metatranscriptomic analysis revealed subtle shifts in the microbiome of RS. These results contribute to a deeper understanding of acetamiprid adaptation by the pea aphid and provide new insights for aphid control strategies.

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“…In the present study, we aimed to determine the biochemical basis of resistance in a Vip3A-resistant H. virescens colony. In a previous study with this colony (Vip-Sel), resistance was shown to be polygenic (18), and a transcriptomic analysis showed significant differential gene expression with 420 over-expressed and 1,569 under-expressed genes (19). Results herein support that Vip3Aa binding is not altered significantly in resistant compared to susceptible H. virescens , and demonstrate an association between Vip3A resistance and down-regulation of a membrane bound alkaline phosphatase (mALP).…”

Section: Introductionmentioning

confidence: 81%

Reduced levels of membrane-bound alkaline phosphatase in Vip3Aa-resistantHeliothis virescens

Pinos

Chakroun

Millán-Leiva

et al. 2020

Preprint

Self Cite

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24The Vip3Aa insecticidal protein from Bacillus thuringiensis (Bt) is produced by specific 25 transgenic corn and cotton varieties for efficient control of target lepidopteran pests. The 26 main threat to this technology is the evolution of resistance in targeted insect pests, thus 27 understanding the mechanistic basis of resistance is crucial to deploy the most appropriate 28 strategies for resistance management. In this work, a laboratory-selected colony of 29Heliothis virescens (Vip-Sel) highly resistant to the Vip3Aa protein was used to test 30 whether an alteration of membrane receptors in the insect midgut might explain the 31 resistance phenotype. Binding of 125 I-labeled Vip3Aa to brush border membrane vesicles 32 (BBMV) from 3rd instar larvae from Vip-Sel was not significantly different from binding 33 in the reference susceptible colony. Interestingly, BBMV from Vip-Sel larvae show 34 dramatically reduced levels of alkaline phosphatase activity, which was further confirmed 35 by a strong down-regulation of the membrane-bound alkaline phosphatase 1 (HvmALP1) 36 gene. However, its involvement as a receptor for the Vip3Aa protein was not supported 37 by ligand blotting and viability assays with insect cells expressing HvmALP1. These data 38 support that reduced alkaline phosphatase, previously observed in insect colonies 39 resistant to Cry proteins from Bt, may also serve as an indirect marker that is not 40 mechanistically involved in resistance to Vip3Aa. 41 42 IMPORTANCE 43The Vip3Aa insecticidal protein remains the only lepidopteran-specific trait in transgenic 44Bt crops with no cases of field-evolved resistance. While laboratory-selected resistance 45 to Vip3A has been reported elsewhere, the mechanism for resistance is unknown. Results 46 in this work show lack of significant Vip3Aa binding alterations in resistant and reference 47 595 596

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HT‐SuperSAGE of the gut tissue of a Vip3Aa‐resistant Heliothis virescens (Lepidoptera: Noctuidae) strain provides insights into the basis of resistance

Cited by 8 publications

References 81 publications

Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony

Reduced Membrane-Bound Alkaline Phosphatase Does Not Affect Binding of Vip3Aa in a Heliothis virescens Resistant Colony

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid <em>Acyrthosiphon pisum</em> (Hemiptera: Aphididae) to Acetamiprid

Reduced levels of membrane-bound alkaline phosphatase in Vip3Aa-resistantHeliothis virescens

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