HTR8/SVneo, but not JEG3, cells display trophoblast progenitor cell-like characteristics indicative of self-renewal, repopulation activity and expression of “stemness”- associated transcription factors

Weber, Maja; Knoefler, Ilka; Schleußner, E; Markert, Udo R.; Fitzgerald, Justine S.

doi:10.1016/j.placenta.2013.06.264

Cited by 3 publications

(4 citation statements)

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“…These studies range from the use of a single cell line, most commonly, HTR8/SVneo (Graham et al, 1993) to ex vivo and in vivo models with contradictory findings are often reported. As the HTR8/SVneo cells in different labs appear to be cultured under different conditions and gain different characteristics (Kilburn et al, 2000;Takao et al, 2011;Weber et al, 2013;Lee et al, 2016;Abou-Kheir et al, 2017;Brkić et al, 2020), it is important to use multiple experimental models when studying trophoblast behaviours.…”

Section: Microrna Regulation Of Trophoblast Invasion Spiral Artery Re...mentioning

confidence: 99%

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

Hayder

Shan²,

Chen³

et al. 2022

Front. Cell Dev. Biol.

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It is now well-established that microRNAs (miRNAs) are important regulators of gene expression. The role of miRNAs in placental development and trophoblast function is constantly expanding. Trophoblast invasion and their ability to remodel uterine spiral arteries are essential for proper placental development and successful pregnancy outcome. Many miRNAs are reported to be dysregulated in pregnancy complications, especially preeclampsia and they exert various regulatory effects on trophoblasts. In this review, we provide a brief overview of miRNA biogenesis and their mechanism of action, as well as of trophoblasts differentiation, invasion and spiral artery remodeling. We then discuss the role of miRNAs in trophoblasts invasion and spiral artery remodeling, focusing on miRNAs that have been thoroughly investigated, especially using multiple model systems. We also discuss the potential role of miRNAs in the pathogenesis of preeclampsia.

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Section: Microrna Regulation Of Trophoblast Invasion Spiral Artery Re...mentioning

confidence: 99%

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

Hayder

Shan²,

Chen³

et al. 2022

Front. Cell Dev. Biol.

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“…Briefly, 2x10 5 cells per 20 𝜇L drop supplemented RPMI-1640 with %10 FBS and %1 Pen-Strep were plated onto the lid of Petri dishes in regular arrays (20 drops/Petri lid). The lid was inverted over the bottom of the PBS-filled Petri dish (Weber et al, 2013) (Figure 1). The petri dish with the hanging drops were cultured under standard conditions (37 o C, 5% CO2, humidified atmosphere) for 240 hours to imitate human 5th day blastocyst size (170-220 µm).…”

Section: Spheroid Formation With Hanging Dropsmentioning

confidence: 99%

“…In another study, it was determined that the cell line expressed genes with stem cellular characteristics such as SOX2 and NANOG. It has been attested that the trophoblast stem cell marker CDX2 and the human embryonic stem cell marker NOTCH1 are positive (Weber et al, 2013). In studies conducted, when the cell line was examined and the receptors of the cells were evaluated, the model exactly reflects the reality in imitating the implantation stage of a healthy human embryo (Hannan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death

Garip¹,

Özdil²,

Kocaturk-Calik³

et al. 2022

Acta Histochemica

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“…Briefly, 2x10 5 cells per 20 L drop supplemented RPMI-1640 with %10 FBS and %1 Pen-Strep were plated onto the lid of Petri dishes in regular arrays (20 drops/Petri lid). The lid was inverted over the bottom of the PBS-filled Petri dish (Weber et al, 2013) (Figure 1). The petri dish with the hanging drops were cultured under standard conditions (37 o C, 5% CO2, humidified atmosphere) for 240 hours to imitate human 5th day blastocyst size (170-220 µm).…”

Section: Spheroid Formation With Hanging Dropsmentioning

confidence: 99%

Effect of Endoplasmic Reticulum Stress on Human Trophoblast Cells: Survival Triggering or Catastrophe Resulting in Death

Garip

Özdil

Calik-Kocaturk

et al. 2021

Preprint

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Although in vitro endoplasmic reticulum (ER) stress studies have been carried out using Tunicamycin in human trophoblast cell lines in recent years, the effect of calcium homeostasis impaired by the effect of Thapsigargin on cell survival - death pathways have not been clearly demonstrated. Here, the effects of ER stress and impaired calcium homeostasis on cell death pathways such as apoptosis and autophagy in 2-dimensional and 3-dimensional cell cultures were investigated using the HTR8 / SVneo cell line representing human trophoectoderm cells and the ER stressor Thapsigargin. By using Real Time PCR, gene and immunofluorescence analyzes were studied at the protein level. In this study, it has been established that the Thapsigargin creates ER stress by increasing the level of GRP78 gene and protein in 2 and 3 dimensions of human trophoectoderm cells and that cells show different characterization properties in 2 and 3 dimensions. It has been determined that while it moves in the direction of EIF2A and IRE1A mechanisms in 2 dimensions, it proceeds in the direction of EIF2A and ATF6 mechanisms in 3 dimensions and creates different responses in survival and programmed cell death mechanisms such as apoptosis and autophagy. With forthcoming studies, it is thought that the effects of Thapsigargin on the intrinsic pathway of apoptosis and the linkage of the autophagy mechanism, the examination of the survival-death pathways in the co-culture model with endometrial cells, therapeutic target molecules that will contribute to the elucidation of intracellular cell dynamics may increase the success of implantation.

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HTR8/SVneo, but not JEG3, cells display trophoblast progenitor cell-like characteristics indicative of self-renewal, repopulation activity and expression of “stemness”- associated transcription factors

Cited by 3 publications

References 0 publications

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia

Effect of endoplasmic reticulum stress on human trophoblast cells: Survival triggering or catastrophe resulting in death

Effect of Endoplasmic Reticulum Stress on Human Trophoblast Cells: Survival Triggering or Catastrophe Resulting in Death

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