Huaier aqueous extract suppresses human breast cancer cell proliferation through inhibition of estrogen receptor α signaling

Wang, Xiaolong; Zhang, Ning; Huo, Qiang; Sun, Mingxia; Lv, Shangge; Yang, Qifeng

doi:10.3892/ijo.2013.1947

Cited by 31 publications

(28 citation statements)

References 39 publications

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“…The nuclear factor κB (NF-κB) pathway, an essential pathway for tumorigenesis (28), is involved in this process. Following Huaier treatment, phosphorylated levels of p65 were reduced almost to the basal level without estrogen, suggesting that Huaier extract abolished the effect of estrogen on the activation of NF-κB and thus suppressed the proliferation of breast cancer cells induced by estrogen (27).…”

Section: Anti-proliferative Effect Of Huaiermentioning

confidence: 93%

“…A recent study found that Huaier extract efficiently inhibited estrogen- stimulated proliferation in three ERα-positive breast cancer cell lines (MCF-7, T47D and ZR-75-1), confirmed by marked downregulation of ERα mRNA and protein levels in Huaiertreated cells. This process was associated with activation of the proteasome (27). The nuclear factor κB (NF-κB) pathway, an essential pathway for tumorigenesis (28), is involved in this process.…”

Section: Anti-proliferative Effect Of Huaiermentioning

confidence: 99%

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The anticancer effect of Huaier (Review)

Song

Zhang

2015

Oncology Reports

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Abstract. Trametes robiniophila Murr. (Huaier) is a sandy beige mushroom found on the trunks of trees and has been widely used in traditional Chinese medicine (TCM) for ~1,600 years. The anticancer effects of Huaier have attracted increasing worldwide interest in recent years. Accumulating evidence suggests that the anticancer mechanism of Huaier may be associated with various biological activities, such as inhibition of cell proliferation, anti-metastasis, interference with tumor angiogenesis and tumor-specific immunomodulatory effect. Animal and experimental studies suggest that Huaier is a promising anticancer agent. Further clinical research is warranted to illustrate the untapped chemopreventive and therapeutic potential of Huaier either alone or in conjunction with existing therapies.

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Section: Anti-proliferative Effect Of Huaiermentioning

confidence: 93%

Section: Anti-proliferative Effect Of Huaiermentioning

confidence: 99%

The anticancer effect of Huaier (Review)

Song

Zhang

2015

Oncology Reports

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“…Huaier extract has been used in China for approximately 1600 years [4]; however, its anti-tumor effects were confirmed more recently [8, 13, 41]. We have previously demonstrated that Huaier extract induces autophagy and apoptosis [4, 11, 31], regulates tumor-associated macrophages [10], inhibits cancer stem cells [12], and suppresses the ERa signaling pathway [13]. However, its underlying mechanisms were not completely understood.…”

Section: Discussionmentioning

confidence: 99%

Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway

Wang

Chen

et al. 2017

Cell Physiol Biochem

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Background/Aims: Increasing evidence indicates that Huaier extract has promising therapeutic effects against cancer. However, the mechanisms that underlie its anti-tumor effects remain unclear. In recent years, various studies have shown that long noncoding RNAs (lncRNAs) play a critical role in the regulation of cancer development and progression. Here, we explored the role of lncRNAs in Huaier-induced tumor suppression. Methods: Microarray profiling was performed to identify the candidate lncRNAs affected by Huaier extract. Quantitative realtime PCR (qPCR) was used to evaluate the transfection efficiency and the influence of Huaier extract on H19 expression. The effect of Huaier extract on the cell viability was examined by MTT. Moreover, the rates of apoptotic cells were detected using flow-cytometric analysis. Western blot analysis was applied to show the protein levels of CBL. Results: Microarray data derived from Huaier-treated breast cancer cells identified H19 as a potential target. Huaier extract reduced the expression of H19. The over-expression of H19 inhibited the cytotoxic effects of Huaier extract; in contrast, reduced H19 expression enhanced the function of Huaier extract. MiR-675-5p was identified as a mature product of H19. Moreover, Huaier extract reduced the miR-675-5p expression. Upregulating miR-675-5p reversed the inhibitory effects of Huaier extract, whereas downregulating miR-675-5p sensitized breast cancer cells to the effect of Huaier extract. In addition, Huaier extract increased the expression of CBL protein, a direct target of miR-675-5p. Conclusion: Collectively, the data demonstrate that Huaier extract reduces viability and induces apoptosis in breast cancer cells via H19-miR-675-5p-CBL axis regulation.

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“…Xie HX et al and Zhang F et al reported that Huaier induced cell cycle arrest at the G2 phase in the gastric cancer MKN45 and SGC7901 cells and melanoma A875 cell line [15,17]. Wang X et al and Zhang N et al reported that cellcycle arrest occurs in breast cancer MCF-7 and MDA-MB-231 cells and human umbilical vein endothelial cells in the G0/G1 phase [16,31]. Zhang C found that Huaier extract induces hepatocellular carcinoma cell HepG2 and Bel-7402 arrest in S phase [32].…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 99%

Huang Qi Huai Granules Induce Apoptosis in Acute Lymphoblastic Leukemia Cells through the Akt/FoxO1 Pathway

Han¹,

Lin²,

Zeng³

et al. 2016

Cell Physiol Biochem

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Background/Aims: In recent years, a traditional Chinese medicine named Huang Qi Huai (HQH) has been frequently used in China for solid tumor therapy. However, the role of HQH on leukemia cells and its underlying mechanisms have not been elucidated. In this study, we investigated the effect of HQH on the proliferation and apoptosis of acute lymphoblastic leukemia (ALL) cell lines. Methods: Sup-B15 and Nalm-6 cells were treated with gradient doses of HQH for 24, 48 or 72 h. Cell viability was measured using a CCK8 assay and cell cycle distribution and apoptosis levels were analyzed using flow cytometry. Western blotting was used to assess the levels of proteins associated with the apoptotic pathway. Results: The results revealed that cell survival decreased significantly with increasing concentrations of HQH. HQH induced G2 cell-cycle arrest and cell apoptosis in a dose-dependent manner. HQH inhibited phosphorylated-Akt, phosphorylated- FoxO1 and Bcl2 expression and upregulated Bim, cleaved-caspase-3 and Bax expression in a dose-dependent manner, which suggests that HQH induces the apoptosis of ALL cells via the Akt/FoxO1 pathway. Conclusion: HQH is a potential complementary agent for the treatment of acute lymphoblastic leukemia.

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Huaier aqueous extract suppresses human breast cancer cell proliferation through inhibition of estrogen receptor α signaling

Cited by 31 publications

References 39 publications

The anticancer effect of Huaier (Review)

The anticancer effect of Huaier (Review)

Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway

Huang Qi Huai Granules Induce Apoptosis in Acute Lymphoblastic Leukemia Cells through the Akt/FoxO1 Pathway

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