Huangqi decoction alleviates dimethylnitrosamine-induced liver fibrosis: An analysis of bile acids metabolic mechanism

Song, Yanan; Zhang, Gui-biao; Lu, Yi‐Yu; Chen, Qilong; Yang, Li; Wang, Zheng-Tao.; Liu, Ping; Su, Shi‐Bing

doi:10.1016/j.jep.2016.05.040

Cited by 41 publications

(22 citation statements)

References 38 publications

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“…In these experiments, 16 individual bile acids were profiled by LC-MS and demonstrated that bile acids were elevated by DMN treatment and that HQD restored these to normal levels. Additionally, gene expression related to bile acid synthesis and transport was examined, and also altered by DMN treatment, but restored by HQD [167].…”

Section: Fibrosis and Cirrhosismentioning

confidence: 99%

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Beyoğlu

Idle

2020

Metabolites

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In recent years, there has been a plethora of attempts to discover biomarkers that are more reliable than α-fetoprotein for the early prediction and prognosis of hepatocellular carcinoma (HCC). Efforts have involved such fields as genomics, transcriptomics, epigenetics, microRNA, exosomes, proteomics, glycoproteomics, and metabolomics. HCC arises against a background of inflammation, steatosis, and cirrhosis, due mainly to hepatic insults caused by alcohol abuse, hepatitis B and C virus infection, adiposity, and diabetes. Metabolomics offers an opportunity, without recourse to liver biopsy, to discover biomarkers for premalignant liver disease, thereby alerting the potential of impending HCC. We have reviewed metabolomic studies in alcoholic liver disease (ALD), cholestasis, fibrosis, cirrhosis, nonalcoholic fatty liver (NAFL), and nonalcoholic steatohepatitis (NASH). Specificity was our major criterion in proposing clinical evaluation of indole-3-lactic acid, phenyllactic acid, N-lauroylglycine, decatrienoate, N-acetyltaurine for ALD, urinary sulfated bile acids for cholestasis, cervonoyl ethanolamide for fibrosis, 16α-hydroxyestrone for cirrhosis, and the pattern of acyl carnitines for NAFL and NASH. These examples derive from a large body of published metabolomic observations in various liver diseases in adults, adolescents, and children, together with animal models. Many other options have been tabulated. Metabolomic biomarkers for premalignant liver disease may help reduce the incidence of HCC.

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Section: Fibrosis and Cirrhosismentioning

confidence: 99%

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Beyoğlu

Idle

2020

Metabolites

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“…Experimental studies have revealed that HQD elicits a remarkable antifibrosis effect. [6][7][8][9] Analyses of individual compounds and herbs within this formulation have been previously studied in terms of its chemical composition and bioactive compound quantification. [10][11][12] However, no PK studies have been conducted on the unique formulation of HQD in humans.…”

Section: Study Highlightsmentioning

confidence: 99%

“…Huangqi decoction (HQD), a classical formula for treating liver injury in China, is composed of Radix Astragali and Radix Glycyrrhizae , mixed in a ratio of 6/1 (wt/wt). Experimental studies have revealed that HQD elicits a remarkable antifibrosis effect . Analyses of individual compounds and herbs within this formulation have been previously studied in terms of its chemical composition and bioactive compound quantification .…”

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confidence: 99%

Poly‐pharmacokinetic Study of a Multicomponent Herbal Medicine in Healthy Chinese Volunteers

Xie

Wang

Zhang

et al. 2017

Clin Pharma and Therapeutics

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The advent of mass spectrometry-based analytical technologies coupled with multivariate statistical methods offer tremendous new opportunities for understanding the pharmacokinetics (PKs) of multicomponent herbal medicines (HMs). We recently proposed a poly-PK strategy to characterize the concentration-time profile and the metabolic response profile of multicomponent HMs using an integrated phytochemical and metabolomics approach. Here, we provided the first example of the poly-PK strategy, in which we simultaneously characterized the PK as well as the metabolic response profiles of a Chinese HM, Huangqi decoction (HQD, consisting of Radix Astragali and Radix Glycyrrhizae), in healthy Chinese volunteers. Using the poly-PK approach, we identified 56 HQD-derived compounds and 292 biotransformed HQD metabolites in human plasma. Additionally, we acquired the concentration-time profiles of these plasma HQD metabolites and correlated them with a plasma metabolomics profile consisting of 166 human endogenous metabolites that were significantly altered in response to HQD intervention.

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“…In recent years, studies have shown that multiple components of TCM formulae acting on multiple targets are responsible for their therapeutic effects (Chen, Chen, Lu, Wang, & Wang, ; Li et al, ; Song et al, ). A pharmacokinetic study of the multiple components in plasma is an important method for studying the bioactive components of TCM formulae.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple components in rat plasma by UPLC‐MS/MS and pharmacokinetic study after oral administration of Huangqi decoction

Zeng

Wang

et al. 2018

Biomedical Chromatography

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A rapid, sensitive and accurate UPLC-MS/MS method was developed for the simultaneous quantification of components of Huangqi decoction (HQD), such as calycosin-7-O-β-d-glucoside, calycosin-glucuronide, liquiritin, formononetin-glucuronide, isoliquiritin, liquiritigenin, ononin, calycosin, isoliquiritigenin, formononetin, glycyrrhizic acid, astragaloside IV, cycloastragenol, and glycyrrhetinic acid, in rat plasma. After plasma samples were extracted by protein precipitation, chromatographic separation was performed with a C column, using a gradient of methanol and 0.05% acetic acid containing 4mm ammonium acetate as the mobile phase. Multiple reaction monitoring scanning was performed to quantify the analytes, and the electrospray ion source polarity was switched between positive and negative modes in a single run of 10 min. Method validation showed that specificity, linearity, accuracy, precision, extraction recovery, matrix effect and stability for 14 components met the requirements for their quantitation in biological samples. The established method was successfully applied to the pharmacokinetic study of multiple components in rats after intragastric administration of HQD. The results clarified the pharmacokinetic characteristics of multiple components found in HQD. This research provides useful information for understanding the relation between the chemical components of HQD and their therapeutic effects.

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Huangqi decoction alleviates dimethylnitrosamine-induced liver fibrosis: An analysis of bile acids metabolic mechanism

Cited by 41 publications

References 38 publications

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy

Poly‐pharmacokinetic Study of a Multicomponent Herbal Medicine in Healthy Chinese Volunteers

Simultaneous quantification of multiple components in rat plasma by UPLC‐MS/MS and pharmacokinetic study after oral administration of Huangqi decoction

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