Huangqi Guizhi Wuwu Decoction Improves Arthritis and Pathological Damage of Heart and Lung in TNF-Tg Mice

Wang, Yi; Chen, Tao; Chao, Yang; Li, Qiang; Ma, Mengjiao; Xu, Hao; Shi, Qi; Wang, Yongjun; Wang, Youhua; Liang, Qianqian

doi:10.3389/fphar.2022.871481

Cited by 16 publications

(11 citation statements)

References 57 publications

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“…According to numerous studies [ 84 – 88 ], the progression of these diseases is closely associated with macrophage polarization. According to studies, HQGZWWD may improve rheumatoid arthritis and atherosclerosis by improving the level of inflammation [ 89 – 91 ], but it remains to be determined whether it can exert an anti-inflammatory effect by regulating macrophage polarization. Currently, there is no research on HQGZWWD in the treatment of insulin resistance, asthma, or Alzheimer’s disease.…”

Section: Discussionmentioning

confidence: 99%

Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology

sutong

Wang

et al. 2023

BMC Complement Med Ther

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Background Huangqi Guizhi Wuwu decoction (HQGZWWD) is a traditional Chinese herbal medicine formulation with significant anti-inflammatory activity. However, its underlying mechanism remains unknown. Through network pharmacology and experimental validation, this study aimed to examine the potential mechanism of HQGZWWD in regulating macrophage polarization and inflammation. Methods The active components were obtained from the Traditional Chinese Medicine Systems Pharmacology database and Analysis Platform (TCMSP), whereas the corresponding targets were obtained from the TCMSP and Swiss Target Prediction database. The GeneCards database identified targets associated with macrophage polarization and inflammation. Multiple networks were developed to identify the key compounds, principal biological processes, and pathways of HQGZWWD that regulate macrophage polarization and inflammation. Autodock Vina is utilized to assess the binding ability between targets and active compounds. Finally, confirm the experiment’s central hypothesis. Human histiocytic lymphoma (U-937) cells were transformed into M1 macrophages following stimulation with Lipopolysaccharide (LPS) to evaluate the effect of HQGZWWD drug-containing mouse serum (HQGZWWD serum) on regulating macrophage polarization and inflammation. Results A total of 54 active components and 859 HQGZWWD targets were obtained. There were 9972 targets associated with macrophage polarization and 11,109 targets associated with inflammation. After screening, 34 overlapping targets were identified, of which 5 were identified as central targets confirmed by experiments, including the α7 nicotinic acetylcholine receptor (α7 nAchR), interleukin 6 (IL-6), Interleukin-1 beta (IL-1β), interleukin 10 (IL-10) and growth factor beta (TGF-β1). Pathway enrichment analysis revealed that 34 overlapping targets were enriched in multiple pathways associated with macrophage polarization and inflammation, including the TGF beta signaling pathway, NF-kappa B signaling pathway, JAK-STAT signaling pathway, and TNF signaling pathway. Molecular docking confirmed that the majority of HQGZWWD’s compounds can bind to the target. In vitro experiments, HQGZWWD serum was shown to up-regulate the expression of α7 nAchR, reduce the number of M1 macrophages, stimulate the production of M2 macrophages, inhibit the expression of pro-inflammatory cytokines IL-6 and IL1-β, and increase the expression of anti-inflammatory cytokines IL-10 and TGF-β1. Conclusion HQGZWWD can regulate the number of M1/M2 macrophages and the level of inflammatory cytokines, and the underlying mechanism may be related to the up-regulation of α7 nAchR expression.

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Section: Discussionmentioning

confidence: 99%

Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology

sutong

Wang

et al. 2023

BMC Complement Med Ther

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“…Traditional Chinese medicine has unique advantages in the prevention and treatment of stroke, which has the characteristics of multi-target and multi-pathway synergism, and has become a part of the clinical treatment of stroke in China. Modern pharmacology studies (Lv et al, 2021; Y. Wang et al, 2022); Li et al, 2006) found that HGWD on anti-inflammation, analgesia, anti-oxidation, regulating immune function, improving microcirculation, and blood rheology properties play an essential role in such aspects.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology-based Analysis of the Effects of Huangqi Guizhi Wuwu Decoction on Ischemic Stroke^*

Gong,

Zhou,

Xia

et al. 2023

Pharmacognosy Magazine

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Background The purpose of this study was to investigate the molecular mechanisms and material basis of Huangqi Guizhi Wuwu Decoction (HGWD) in treating clinical Ischemic stroke (IS) using network pharmacology and molecular docking techniques. Materials and Methods The study retrieved and screened the practical components of HGWD from the TCMSP, BATMAN-TCM, and ETCM databases. Target prediction was performed using the SwissTargetPrediction database, and disease targets for IS were obtained from the Gene Cards, DisGeNET, and OMIM databases. The regulatory targets for HGWD in treating IS were obtained by VENN analysis, and the protein-protein interaction network of disease targets was constructed using the STRING database. GO analysis and KEGG enrichment analysis were performed using the DAVID database. Results A total of 227 active ingredients and 354 drug targets were obtained for HGWD, and after combining them with 4,402 disease targets, 253 potential targets for HGWD to treat IS were identified. GO and KEGG enrichment analyses yielded 251 gene functions and 422 pathways. The study found that the active components in HGWD may treat IS through the IL-17 signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, and other targets. Conclusion The study identified potential molecular mechanisms and the material basis of HGWD in treating clinical IS. The results suggest that HGWD could be a promising therapeutic approach for treating IS, and further experimental validation is needed.

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“…TNFtransgenic (TNF-Tg) mice express the human TNF-α gene on a C57BL/6 background and spontaneously develop erosive polyarthritis with several characteristics observed in patients with RA, as well as the pathological phenotypes of cardiopulmonary complications [25] . Female TNF-Tg mice are useful tools for dissecting the molecular mechanisms of the pathogenic process and evaluating the efficacy of novel therapeutic strategies for RA [26][27][28] and the cardiopulmonary complications of RA [29][30][31] . In the present study, we used TNF-Tg mice to evaluate the protective effect of DGNTT against the cardiopulmonary complications of RA.…”

Section: Introductionmentioning

confidence: 99%

Danggui Niantong decoction ameliorates joint inflammation and cardiopulmonary injury in TNF-Tg mice

Yang,

Chen,

et al. 2023

Acupuncture and Herbal Medicine

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Objective: Rheumatoid arthritis (RA) is a common autoimmune disease characterized by multiple joint lesions and systemic complications. Danggui Niantong Decoction (DGNTT) has been clinically used for RA treatment; however, its beneficial effect on cardiopulmonary complications has not been reported. Methods: Female tumor necrosis factor-transgenic (TNF-Tg) mice were used to evaluate the therapeutic effects of DGNTT on arthritis and cardiopulmonary complications. Methotrexate (MTX) served as a positive control. Histopathological assessment of the joint sections was performed using hematoxylin and eosin (H&E), Alcian Blue/Orange G (ABOG), and tartrate-resistant acid phosphatase (TRAP) staining. Bone mass was assessed by micro-computed tomography (CT), inflammatory infiltrates in the heart and lungs were evaluated by H&E staining, cardiopulmonary fibrotic injury was identified by Masson’s trichrome staining, and hypertrophy of mouse cardiomyocytes was measured by wheat germ agglutinin (WGA) staining. Results: DGNTT mitigated the inflammation of the ankle joint synovium, decreased the number of osteoclasts, and increased the area of cartilage and bone mass in TNF-Tg mice. In addition, DGNTT decreased the infiltration of inflammatory cells into the lung and heart tissues, accompanied by a reduction in cardiopulmonary fibrosis and myocardial cell hypertrophy in TNF-Tg mice. As a positive control drug, MTX attenuated the pathological changes in joints, but had no beneficial effect on cardiopulmonary inflammation and fibrosis in TNF-Tg mice. Conclusion: DGNTT improved joint lesions and alleviated cardiopulmonary complications in TNF-Tg mice.

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Huangqi Guizhi Wuwu Decoction Improves Arthritis and Pathological Damage of Heart and Lung in TNF-Tg Mice

Cited by 16 publications

References 57 publications

Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology

Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology

Network Pharmacology-based Analysis of the Effects of Huangqi Guizhi Wuwu Decoction on Ischemic Stroke^*

Danggui Niantong decoction ameliorates joint inflammation and cardiopulmonary injury in TNF-Tg mice

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Huangqi Guizhi Wuwu Decoction Improves Arthritis and Pathological Damage of Heart and Lung in TNF-Tg Mice

Cited by 16 publications

References 57 publications

Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology

Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology

Network Pharmacology-based Analysis of the Effects of Huangqi Guizhi Wuwu Decoction on Ischemic Stroke*

Danggui Niantong decoction ameliorates joint inflammation and cardiopulmonary injury in TNF-Tg mice

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Network Pharmacology-based Analysis of the Effects of Huangqi Guizhi Wuwu Decoction on Ischemic Stroke^*