Huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

Abstract

Hepatocellular carcinoma (HCC) is a malignancy with a high incidence and fatality rate worldwide. Hepatitis B virus (HBV) infection is one of the most important risk factors for its occurrence and development. Early detection of HBV-associated HCC (HBV-HCC) can improve clinical decision-making and patient outcomes. Biomarkers are extremely helpful, not only for early diagnosis, but also for the development of therapeutics. MicroRNAs (miRNAs), a subset of non-coding RNAs approximately 22 nucleotides in length, have increasingly attracted scientists’ attention due to their potential utility as biomarkers for cancer detection and therapy. HBV profoundly impacts the expression of miRNAs potentially involved in the development of hepatocarcinogenesis. In this review, we summarize the current progress on the role of miRNAs in the diagnosis and treatment of HBV-HCC. From a molecular standpoint, we discuss the mechanism by which HBV regulates miRNAs and investigate the exact effect of miRNAs on the promotion of HCC. In the near future, miRNA-based diagnostic, prognostic, and therapeutic applications will make their way into the clinical routine.

Tenovin-1, a Selective SIRT1/2 Inhibitor, Attenuates High-fat Diet-induced Hepatic Fibrosis via Inhibition of HSC Activation in ZDF Rats

“…HFD caused severe disruption of cellular structures in the rat liver, accompanied with histological and morphological changes such as microvascular and macrovascular steatosis, cytoplasmic vacuolation and hepatocyte hypertrophy. Rats also exhibit hepatocellular ballooning degeneration and inflammatory cell infiltration, indicating a fibrosis-like pathology 35 , 36 . Enlargement and hyperplasia of the liver vasculature was also observed.…”

Silibinin Targeting Heat Shock Protein 90 Represents a Novel Approach to Alleviate Nonalcoholic Fatty Liver Disease by Simultaneously Lowering Hepatic Lipotoxicity and Enhancing Gut Barrier Function

“…As previously mentioned, a specific combination of sodium oleate and sodium palmitate, forming FFA, was utilized to induce intracellular fat accumulation . HepG2 cells, procured from the Chinese Academy of Cell Resource Center (Shanghai, China), were cultured in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin at 37 °C in a humidified 5% CO 2 environment.…”

“…Subsequently, a reverse transcription reaction system (Servicebio) was used to process the RNA samples. Quantitative RT-PCR was performed using an RT-PCR system (Biorad, CA, USA), following the methodology described in our previous investigation . The primer sequences were designed by Servicebio (Table ).…”

“…As previously mentioned, a specific combination of sodium oleate and sodium palmitate, forming FFA, was utilized to induce intracellular fat accumulation. 26…”

“…As previously mentioned, a specific combination of sodium oleate and sodium palmitate, forming FFA, was utilized to induce intracellular fat accumulation. 26 HepG2 cells, procured from the Chinese Academy of Cell Resource Center (Shanghai, China), were cultured in DMEM supplemented with 10% FBS and 1% penicillin−streptomycin at 37 °C in a humidified 5% CO 2 environment. For experimental interventions, HepG2 cells in a serum-free medium at approximately 70% confluency for 12 h were subjected to treatment with either asiatic acid (20 μM) or silibinin (20 μM) in the presence of 1 mM FFA for 24 h. HepG2 cells grown in serum-free medium supplemented with 5% BSA served as the blank control.…”