Huayu Tongluo Recipe Attenuates Renal Oxidative Stress and Inflammation through the Activation of AMPK/Nrf2 Signaling Pathway in Streptozotocin- (STZ-) Induced Diabetic Rats

Li, Yachun; Guo, Shanchun; Yang, Fan; Liu, Lifei; Chen, Zhiqiang

doi:10.1155/2021/5873007

Cited by 11 publications

(11 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 28 , 29 Persistent hyperglycemia induced by STZ was confirmed as T2DM stage, 12 , 13 and abnormal renal function due to podocyte injury was considered the DN stage. 14 Serum TNF-α and IL-1β were tracked and compared in the above three stages.…”

Section: Discussionmentioning

confidence: 99%

“…The 24 h urine volume (UV) was recorded, and the collected urine was stored in a −80°C freezer for later analysis. The 24h-UP and urine creatinine (UCr) levels were measured with commercial kits (Jiancheng Bioengineering Company, Nanjing, China), and the serum SCr and BUN levels 14 were determined with AU5800 ISE biochemistry analyzers (Beckman Coulter, Germany).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters

Wang¹,

Feng²,

Zhang³

et al. 2022

JIR

View full text Add to dashboard Cite

Purpose Diabetic nephropathy (DN) is a serious microvascular complication in those with type 2 diabetes mellitus (T2DM). Evidence confirms that serum tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the T2DM stage are proposed as prognostic markers for DN development, but it is unclear how they affect renal podocyte-associated nephrin and WT-1 expression. In the presence of podocyte injury, glomerular vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS) and hemodynamic parameters are dysregulated. The current research aimed to clarify the relationship of TNF-α and IL-1β with podocyte injury by altering VEGF/eNOS expression and hemodynamic parameters. Methods A high-fat diet/streptozotocin-induced DN rat model was established. Serum TNF-α and IL-1β levels were tracked in the pre-T2DM, T2DM and DN stages. In the DN stage, the mRNA and protein expression levels of renal TNF-α, IL-1β, VEGF, eNOS, nephrin and WT-1 were studied. Renal hemodynamic parameters, including peak systolic velocity, end-diastolic flow velocity and mean velocity were measured with a color Doppler ultrasound technique. Results Compared to those in the normal control (CTL) group, serum TNF-α and IL-1β levels increased significantly in the pre-T2DM stage (obesity, insulin resistance and hyperlipidemia), T2DM stage (hyperglycemia) and DN stage (abnormal renal functions) (all: P < 0.05) in the DN group. Serum TNF-α and IL-1β levels in the T2DM stage were significantly higher than those in the pre-T2DM stage (two: P < 0.05). Compared to the CTL group, renal nephrin, WT-1, TNF-α, IL-1β, eNOS and VEGF expression and hemodynamic parameters in the DN stage all showed significant differences separately (all: P < 0.05). Conclusion Increased serum and renal TNF-α and IL-1β levels played important roles in reducing renal nephrin and WT-1 expression levels, which may be related to the fact that the former affected renal VEGF/eNOS expression and blood flow parameters in the DN rats.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters

Wang¹,

Feng²,

Zhang³

et al. 2022

JIR

View full text Add to dashboard Cite

show abstract

“…There are various pathways like renal apoptosis, oxidative stress, nitrosative stress and inflammation to which the diabetic nephropathy pathogenesis has been tied (Hara et al, 2020; Li et al, 2021; Navarro‐González et al, 2011). Several evidences support an undeniable fact that excessive generation of reactive oxygen species, and hence, oxidative stress is triggered by hyperglycaemia.…”

Section: Discussionmentioning

confidence: 99%

“…Several evidences support an undeniable fact that excessive generation of reactive oxygen species, and hence, oxidative stress is triggered by hyperglycaemia. A critical role is played by oxidative stress in the pathogenesis of diabetic nephropathy (Li et al, 2021). Reports have demonstrated that hyperglycaemia‐induced oxidative and nitrosative stress is responsible for causing inflammation, which activate glomerulus mesangial cells and accelerate renal impairment (Yoh et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

p‐Coumaric acid attenuates high‐fat diet‐induced oxidative stress and nephropathy in diabetic rats

Mani

Kushwaha

Khurana

et al. 2021

Animal Physiology Nutrition

View full text Add to dashboard Cite

Diabetes is emerging as an epidemic disease with an estimation of more than 415 million people already affected, and the figures are expected to grow up to 550 million till 2035 (American Diabetes Association). The various life-threatening complications like nephropathy, neuropathy and retinopathy are usually accomplished with diabetes (Chatterjee et al., 2017). The major cause of renal end-stage disease is diabetic nephropathy which usually developed in the lifetime of 15-40% diabetic patients and is characterised by glomerular atrophy and extracellular matrix accumulation deteriorating kidneys both structurally and functionally (Piccoli et al., 2015;

show abstract

“…Spleen tyrosine kinase are the potential therapeutic target of flavanomarein to ameliorate high glucose-induced extracellular matrix (ECM) against DKD ( Zhang et al, 2020a ). Additionally, Yiqi Jiedu Huayu decoction and Huayu Tongluo recipe suppress diabetic kidney injury in STZ-induced rat model via modulating AMPK pathway ( Li et al, 2021 ; Xuan et al, 2021 ).…”

Section: Therapeutic Mechanism Of Chinese Herbal Medicine In Diabetic...mentioning

confidence: 99%

Therapeutic mechanism and clinical application of Chinese herbal medicine against diabetic kidney disease

Chen

Wu²,

2022

Front. Pharmacol.

View full text Add to dashboard Cite

Diabetic kidney disease (DKD) is the major complications of type 1 and 2 diabetes, and is the predominant cause of chronic kidney disease and end-stage renal disease. The treatment of DKD normally consists of controlling blood glucose and improving kidney function. The blockade of renin-angiotensin-aldosterone system and the inhibition of sodium glucose cotransporter 2 (SGLT2) have become the first-line therapy of DKD, but such treatments have been difficult to effectively block continuous kidney function decline, eventually resulting in kidney failure and cardiovascular comorbidities. The complex mechanism of DKD highlights the importance of multiple therapeutic targets in treatment. Chinese herbal medicine (active compound, extract and formula) synergistically improves metabolism regulation, suppresses oxidative stress and inflammation, inhibits mitochondrial dysfunction, and regulates gut microbiota and related metabolism via modulating GLP-receptor, SGLT2, Sirt1/AMPK, AGE/RAGE, NF-κB, Nrf2, NLRP3, PGC-1α, and PINK1/Parkin pathways. Clinical trials prove the reliable evidences for Chinese herbal medicine against DKD, but more efforts are still needed to ensure the efficacy and safety of Chinese herbal medicine. Additionally, the ideal combined therapy of Chinese herbal medicine and conventional medicine normally yields more favorable benefits on DKD treatment, laying the foundation for novel strategies to treat DKD.

show abstract

Huayu Tongluo Recipe Attenuates Renal Oxidative Stress and Inflammation through the Activation of AMPK/Nrf2 Signaling Pathway in Streptozotocin- (STZ-) Induced Diabetic Rats

Cited by 11 publications

References 55 publications

TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters

TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters

p‐Coumaric acid attenuates high‐fat diet‐induced oxidative stress and nephropathy in diabetic rats

Therapeutic mechanism and clinical application of Chinese herbal medicine against diabetic kidney disease

Contact Info

Product

Resources

About