Background: One of the important factors that affect the quality of nurses’ work is occupational health and safety. One of the occupational diseases is Low Back Pain (LBP). Occupations that have a high risk of back pain are nurses. According to the Indonesian Neurological Association (INA) regarding the incidence of lower back pain in hospital nurses, the prevalence of lower back pain cases in nurses in Indonesia shows that 18,2% occurs in men and 13,6% in women. Then, based on the nursing management report at Bhayangkara Hospital Kendari in 2023, most of the nurses’ complaints were lower back pain due to the workload being too high for nurses. Method: This research was a quantitative analytical research with a cross-sectional design approach. The data analysis was carried out using SPSS statistical tests with multiple linear regression. The population in this study was 102 nurses, while the sample was 102 nurses (total sampling). Results: The results showed a significant p-value for workload 0,049<0,05, physical activity 0,028<0,05, body position 0,004<0,05, working period 0,048<0,05 and body mass index (BMI) 0,001<0,05. Conclusion: This research concluded that there was an effect between workload, physical activity, body position, work period and body mass index on complaints of lower back pain in nurses at Bhayangkara Hospital Tk.III Kendari. This research can be a reference material for hospitals, health workers or nurses and can increase knowledge in preventing or reducing the occurrence of lower back pain while working.