HUCMNCs protect vascular endothelium and prevent ISR after endovascular interventional therapy for vascular diseases in T2DM rabbits

Ding, Hu; Zhang, Tong; Du, Yaping; Liu, Bei; Liu, Yueqin; Wang, Fujun

doi:10.1007/s11010-017-3024-y

Cited by 5 publications

(6 citation statements)

References 43 publications

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“…The injured vascular endothelium can cause vascular inflammation, which accelerates lipid deposition and thrombosis. These changes contribute to neointimal hyperplasia and in-stent restenosis (ISR) [3,4]. The use of drug-eluting stents (DESs) inhibits neointimal hyperplasia but also inhibits reendothelialisation.…”

Section: Introductionmentioning

confidence: 99%

The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries

et al. 2021

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Background Reendothelialisation is the natural pathway that inhibits neointimal hyperplasia and in-stent restenosis. Circulating endothelial progenitor cells (EPCs) derived from bone marrow (BM) might contribute to endothelial repair. However, the temporal and spatial distributions of reendothelialisation and neointimal hyperplasia after EPC transplantation in injured arteries are currently unclear. Methods A carotid balloon injury (BI) model was established in Sprague-Dawley rats, and PKH26-labelled BM-derived EPCs were transplanted after BI. The carotid arteries were harvested on the first, fourth, seventh, and 14th day post-injury and analysed via light-sheet fluorescence microscopy and pathological staining (n = 3). EPC and human umbilical vein endothelial cell culture supernatants were collected, and blood samples were collected before and after transplantation. The paracrine effects of VEGF, IGF-1, and TGF-β1 in cell culture supernatants and serum were analysed by enzyme-linked immunosorbent assay (n = 4). Results Transplanted EPCs labelled with PKH26 were attached to the injured luminal surface the first day after BI. In the sham operation group, the transplanted EPCs did not adhere to the luminal surface. From the fourth day after BI, the mean fluorescence intensity of PKH26 decreased significantly. However, reendothelialisation and inhibition of neointimal hyperplasia were significantly promoted by transplanted EPCs. The degree of reendothelialisation of the EPC7d and EPC14d groups was higher than that of the BI7d and BI14d groups, and the difference in neointimal hyperplasia was observed between the EPC14d and BI14d groups. The number of endothelial cells on the luminal surface of the EPC14d group was higher than that of the BI14d group. The number of infiltrated macrophages in the injured artery decreased in the EPC transplanted groups. Conclusions Transplanted EPCs had chemotactic enrichment and attached to the injured arterial luminal surface. Although decreasing significantly after the fourth day at the site of injury after transplantation, transplanted EPCs could still promote reendothelialisation and inhibit neointimal hyperplasia. The underlying mechanism is through paracrine cytokines and not differentiation into mature endothelial cells.

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Section: Introductionmentioning

confidence: 99%

The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries

et al. 2021

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“…The primary targets of hyperglycemia appear to be the endothelial cells, which may induce endothelial dysfunction and accelerate vasculopathy [1,2]. In an experimental animal model with type 2 diabetes mellitus (T2DM), the remarkable improvement of vascular endothelial function hold promise as therapeutic approaches to decrease the risk of cardiovascular diseases [3,4]. Altered endothelial phenotype is a prominent feature of metabolic disturbance in DM.…”

Section: Introductionmentioning

confidence: 99%

Intravenous Injection of miR-34a Inhibitor Alleviates Diabetes Mellitus-Induced Vascular Endothelial Dysfunction by Targeting NOTCH1

Zhao

Wang

et al. 2018

Exp Clin Endocrinol Diabetes

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“…Thus, how to reduce the restenosis after surgery and keep the target vessel unobstructed in the long term is a clinical problem to be solved urgently. Our research reported that combined with umbilical cord blood stem cell transplantation after intervention could protect vascular endothelial damage and prevent restenosis after intervention 8,9 . It is also found that excimer laser ablation technique was safer in clinical operation and could also be used for subendothelial opening 10 .…”

Section: Introductionmentioning

confidence: 77%

“…Our research reported that combined with umbilical cord blood stem cell transplantation after intervention could protect vascular endothelial damage and prevent restenosis after intervention. 8,9 It is also found that excimer laser ablation technique was safer in clinical operation and could also be used for subendothelial opening. 10 With the development of interventional devices, drug-loaded devices will become the mainstream technology to reduce restenosis after intervention.…”

Section: Introductionmentioning

confidence: 99%

Five‐year follow‐up observation of interventional therapy for lower extremity vascular disease in type 2 diabetes and analysis of risk factors for restenosis

Ding

Hanson

Xing

et al. 2020

Journal of Diabetes

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Background: The high incidence of type 2 diabetes, the low rate of compliance, and the complex mechanism of vascular disease caused by diabetes make its complications increase year by year. Our study aimed to investigate the clinical characteristics of lower extremity vascular diseases in type 2 diabetes and evaluate the long-term efficacy of vascular intervention for these diseases. Methods: From 2007 to 2014, 362 patients who underwent vascular intervention in our hospital due to lower extremity vascular diseases in type 2 diabetes were followed up for 5 years and their clinical characteristics were analyzed in this retrospective study. Results: Compared with those before treatment, the values of blood pressure, fasting blood glucose, glycated hemoglobin (HbA1c), total cholesterol (TC), triglyceride Ester (TG), and low density lipoprotein-cholesterol (LDL-C) of patients were significantly lower 5 years after intervention (P < 0.01). We found that the levels of fibrinogen, blood glucose, HbA1c, TC, TG, LDL-C, and small dense low-density lipoprotein (sdLDL) in the vascular restenosis group were significantly higher than those in the vascular patency group (P < 0.001), whereas the level of HDL-C in the vascular restenosis group was significantly lower compared with the vascular patency group. Conclusions: Vascular intervention can significantly improve a series of biochemical indicators in patients with lower extremity vascular diseases caused by type 2 diabetes. Postoperative restenosis may be related to hypertension, duration of diabetes, rate of inferior knee disease, fibrinogen, and sdLDL. Good survival and limb salvage were achieved in the patients in this series with interventions and medical treatment provided by endocrinologists.

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HUCMNCs protect vascular endothelium and prevent ISR after endovascular interventional therapy for vascular diseases in T2DM rabbits

Cited by 5 publications

References 43 publications

The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries

The effect of endothelial progenitor cell transplantation on neointimal hyperplasia and reendothelialisation after balloon catheter injury in rat carotid arteries

Intravenous Injection of miR-34a Inhibitor Alleviates Diabetes Mellitus-Induced Vascular Endothelial Dysfunction by Targeting NOTCH1

Five‐year follow‐up observation of interventional therapy for lower extremity vascular disease in type 2 diabetes and analysis of risk factors for restenosis

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