HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation

Gradišnik, Lidija; Trapečar, Martin; Rupnik, Marjan Slak; Velnar, Tomaž

doi:10.1007/s00508-015-0771-1

Cited by 14 publications

(13 citation statements)

References 41 publications

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“…First, we removed all noncancer cell types from the cell culture (eg fibroblasts). To achieve this, we developed 2 procedures: (a) the cells were cultivated according to standard cultivation protocols established in our laboratories until the fourth passage (within every passage, cells exhibiting spontaneous epithelial cell growth were preserved; (b) after trypsinization of the primary culture, we removed individual cell groups by pipette and transferred them into a microtiter P24 plates. Both procedures were successful in raising TNBC CLs.…”

Section: List Of Taqman® Assays (Primer‐probe Sets) Used In This Workmentioning

confidence: 99%

Isolation and characterization of the first Slovenian human triple‐negative breast cancer cell line

et al. 2019

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Section: List Of Taqman® Assays (Primer‐probe Sets) Used In This Workmentioning

confidence: 99%

Isolation and characterization of the first Slovenian human triple‐negative breast cancer cell line

et al. 2019

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“…Thus, it is necessary to understand more clearly the survival, proliferation and differentiation of IECs. The isolation and characterization of normal human, porcine and bovine primary intestinal crypt and villus cells have been performed and culture systems established . Fortunately, cultured IECs keep their morphological and functional characteristics during several generations, and thus can be used for subsequent assay.…”

Section: Resultsmentioning

confidence: 99%

Investigation of JAKs/STAT-3 in lipopolysaccharide-induced intestinal epithelial cells

Wei

Zhao

et al. 2016

Clinical and Experimental Immunology

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Janus-activated kinase (JAKs)-signal transducer and activator of transcription 3 (STAT-3) signalling play critical roles in immunoregulation and immunopathology, which involve inflammatory responses and enteritis. JAK phosphorylates STAT-3 in response to stimulation by cytokines or growth factors, and then activates or represses the gene expression. STAT-3 is activated persistently in cancer cells and contributes to the malignant progression of various types of cancer and inflammation. To elucidate the different roles of JAKs in the activation of STAT-3, the lipopolysaccharide-induced primary intestinal epithelial cell (IEC) acute inflammatory model was established. Small interference RNAs (siRNAs) were then employed to attenuate the expression levels of JAKs. Real-time quantitative reverse transcription-polymerase chain reaction (PCR) (qRT-PCR) revealed that JAK mRNA levels were reduced efficiently by JAK-specific siRNAs. Under the IEC inflammatory model transfected with si-JAK, which equates to effective silencing, qRT-PCR and Western blot assays, suggested that knockdowns of JAK attenuated the JAK-induced down-regulation of STAT-3 at the mRNA or protein levels. In particular, JAK1 played a key role, which was consistent with the RNA-Seq results. Subsequently, the expression levels of proinflammatory cytokines interleukin (IL)-1β and tumour necrosis factor (TNF)-α were down-regulated in the IEC inflammatory model transfected with si-JAK1. JAK1 appears as a direct activator for STAT-3, whereas treatments targeting JAK1 repressed STAT-3 sufficiently pathways in the IEC inflammatory model. Therefore, the control of JAK1 using siRNAs has the potential to be an effective strategy against enteritis.

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“…When normal human intestinal epithelial cells (HUIEC, isolated in our lab [53]) were treated under the same experimental conditions, most extracts showed stimulatory effects by increasing cell viability by up to 25% compared to untreated controls ( Figure 5). While only one extract (CAN1) at 10 µg/mL resulted in a modest decrease in cell viability (Figure 5b), the general trend suggests stimulatory effects of Cannabis extracts on cell viability in non-transformed intestinal cells.…”

Section: Stimulatory Effects Of Cannabis Sativa Extracts On Untransfomentioning

confidence: 96%

“…Two cell lines were used for the biological activity analysis of the developed Cannabis extracts: Caco2 (human colorectal adenocarcinoma cells) (ATCC ® HTB-37™) and HU -IEC (human intestinal epithelial cells), previously isolated and characterised by our group [53]. Cells were grown in Dulbecco's modified Eagle's F12 medium (DMEM/F12) supplemented with 10% FBS, 1% L-glutamine, penicillin (100 U/mL) and streptomycin (1 mg/mL) at 37 • C in 5% CO 2 atmosphere in tissue culture flasks.…”

Section: Cell Culturesmentioning

confidence: 99%

Different Cannabis sativa Extraction Methods Result in Different Biological Activities against a Colon Cancer Cell Line and Healthy Colon Cells

Rožanc

Kotnik

Milojević

et al. 2021

Plants

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Cannabis sativa is one of the oldest medicinal plants used by humans, containing hundreds of bioactive compounds. The biological effects and interplay of these compounds are far from fully understood, although the plant’s therapeutic effects are beyond doubt. Extraction methods for these compounds are becoming an integral part of modern Cannabis-based medicine. Still, little is known about how different methods affect the final composition of Cannabis extracts and thus, their therapeutic effects. In this study, different extraction methods were tested, namely maceration, Soxhlet, ultrasound-assisted extraction (UAE), and supercritical CO2 extraction methods. The obtained extracts were evaluated for their cannabinoid content, antioxidant properties, and in vitro bioactivity on human colon cancer and healthy colon cells. Our data suggest that Cannabis extracts, when properly prepared, can significantly decrease cancer cell viability while protecting healthy cells from cytotoxic effects. However, post-processing of extracts poses a significant limitation in predicting therapeutic response based on the composition of the crude extract, as it affects not only the actual amounts of the respective cannabinoids but also their relative ratio to the primary extracts. These effects must be carefully considered in the future preparations of new therapeutic extracts.

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HUIEC, Human intestinal epithelial cell line with differentiated properties: process of isolation and characterisation

Cited by 14 publications

References 41 publications

Isolation and characterization of the first Slovenian human triple‐negative breast cancer cell line

Isolation and characterization of the first Slovenian human triple‐negative breast cancer cell line

Investigation of JAKs/STAT-3 in lipopolysaccharide-induced intestinal epithelial cells

Different Cannabis sativa Extraction Methods Result in Different Biological Activities against a Colon Cancer Cell Line and Healthy Colon Cells

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