HuIFN-γ Antiviral Activity Correlates with Immunological Reactivity in a Double-Monoclonal Antibody Radiometrie Assay

Sedmak, J. James; Siebenlist, Ruth E.; Grossberg, Sidney E.

doi:10.1089/jir.1985.5.397

Cited by 8 publications

(2 citation statements)

References 4 publications

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“…v-lnterferon was determined in the concentrated BAL fluid of the same 10 patients, in which the alveolar macrophage activity could be estimated, by an immunoradiometric assay using reagents obtained from Centocor [20]. A volume of 0.2 ml of each sample (or control standard material) was incubated in the well of a reaction tray along with polystyrene beads coated with a monoclonal anti body against v-interferon for 2 h at room temperature.…”

Section: Ba L Analysismentioning

confidence: 99%

Local Immunity in Patients with Chronic Bronchitis and the Effects of a Bacterial Extract, Broncho-Vaxom<sup>®</sup>, on T Lymphocytes, Macrophages, Gamma-Interferon and Secretory Immunoglobulin A in Bronchoalveolar Lavage Fluid and Other Variables

Emmerich

Emslander²,

Pachmann³

et al. 1990

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In 28 adult patients with nonobstructive chronic bronchitis we investigated components of the immune system of the lower airways and the effects of treatment with Broncho-Vaxom® (BV). An analysis of the washing from bronchoalveolar lavage (BAL) showed, in comparison with healthy controls, an elevation of total cell count (p = 0.003) as well as the Ig A/albumin values (p = 0.02) and a reduction of the macrophage activity (p < 0.001) in patients with chronic bronchitis. After BV a reduction in the total cell count (p = 0.05), an increase in the helper/suppressor T lymphocyte ratio (due mainly to the reduction in the suppressor cells; p = 0.04), a modulation of the IgA/albumin ratio, a stimulation of the impaired alveolar macrophage activity (p = 0.03) and increased concentrations of γ-interferon (p = 0.03) were found in the BAL fluid of patients with chronic bronchitis. The salivary IgA/albumin ratio remained unchanged, the serum IgE concentration fell (p = 0.02) and the urinary IgA concentration rose (p = 0.002). Bronchial mucosa lesions, evaluated endoscopically in terms of structural damage, hyperemia and mucus production, were improved (p < 0.01). These findings indicate that orally administered BV modulates disordered local and systemic immune functions in patients with chronic bronchitis

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Section: Ba L Analysismentioning

confidence: 99%

Local Immunity in Patients with Chronic Bronchitis and the Effects of a Bacterial Extract, Broncho-Vaxom<sup>®</sup>, on T Lymphocytes, Macrophages, Gamma-Interferon and Secretory Immunoglobulin A in Bronchoalveolar Lavage Fluid and Other Variables

Emmerich

Emslander²,

Pachmann³

et al. 1990

Respiration

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“…The gamma interferon concentrations in the specimens were determined with a standard curve. In this test, described by Chang et al (4), the monoclonal antibodies are specific for the epitopes of biological activity of human interferon (23) and can detect titers as low as 0.20 IU/ml.…”

mentioning

confidence: 99%

Presence of gamma interferon in human acute and congenital toxoplasmosis

et al. 1990

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The production of gamma interferon in acute acquired and congenital toxoplasmosis was studied. Gamma interferon was produced at significant titers (P less than 0.001) in the course of both congenital toxoplasmosis and acquired toxoplasmosis at an early stage of infection, when Toxoplasma gondii was multiplying. Its presence in fetal blood was correlated with the positive inoculation of fetal blood or amniotic fluid into mice (95%). The data suggest that the fetus is able to synthesize gamma interferon as early as week 21 of pregnancy. This test, easily and rapidly performed, could be included among those useful for diagnosing fetal toxoplasmic disease.

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