HULC and 7SL RNA expression levels in patients with Crimean‐Congo hemorrhagic fever

Bayyurt, Burcu; Arslan, Serdal; Engin, Aynur; Bakır, Mehmet

doi:10.1002/jmv.25264

Cited by 3 publications

(5 citation statements)

References 24 publications

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“…One could mention that zinc fingers have been shown to play an important role in the development of hepatocellular carcinoma (HCC) [21]. Indeed, CCHFV can cause severe liver injury in humans [22,23]; whether NSm and/or Gn cyto zinc fingers play a role in liver injury in CCHFV infection remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Do NSm virulence factors in the Bunyavirales viral order originate from Gn gene duplication?

Lefebvre

Lin

Cole

et al. 2023

Preprint

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Several viral members of the large viral order of the Bunyavirales carry a NSm protein that acts as a virulence factor. Here we used AlphaFold to predict the structures of these NSm proteins and surprisingly found that the cytosolic domain of the Nairoviridae family NSm (NSmcyto) is predicted to have a very similar fold to the cytosolic domain of glycoprotein N (Gncyto). This observation is particularly striking for CCHFV (a member of the Nairoviridae family), for which the NMR structure of the Gncyto domain has already been described in the literature and shows a double zinc finger. We show that while the sequence identity between the NSmcyto and Gncyto domains is generally weak, the strict conservation of the two zinc-finger forming CCCH motifs in CCHFV NSmcyto explains the full structural conservation predicted by AlphaFold. Interestingly, a similar observation is made for the predictions of NSm in another group of the order Bunyavirales, the family Peribunyaviridae, where the Gncyto structures have not yet been described experimentally. Our findings suggest that NSm is the result of a gene duplication event in these viruses, and indicate that such events may indeed be common in the recent evolutionary history of RNA viruses. Importantly, our predictions provide a first insight into the long-unknown structure of NSm and its link to virulence.

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Section: Discussionmentioning

confidence: 99%

Do NSm virulence factors in the Bunyavirales viral order originate from Gn gene duplication?

Lefebvre

Lin

Cole

et al. 2023

Preprint

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show abstract

“…6 Also, in preliminary studies conducted by our study group, some lncRNAs were investigated using quantitative polymerase chain reaction (qPCR) in the pathogenesis of the CCHF. 7,8 According to the literature so far, there is no study on lncRNA gene expression in CCHF. In the present study, we investigate long noncoding RNA (lncRNA) gene expression profiles in patients with CCHF via microarray analysis for the first time.…”

Section: Introductionmentioning

confidence: 99%

“…Our previous study determined the most important miRNAs of the CCHF pathogenesis 6 . Also, in preliminary studies conducted by our study group, some lncRNAs were investigated using quantitative polymerase chain reaction (qPCR) in the pathogenesis of the CCHF 7,8 . According to the literature so far, there is no study on lncRNA gene expression in CCHF.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA expression analysis in Crimean Congo hemorrhagic fever patients

Arslan

Bakır

Bayyurt

et al. 2022

Journal of Medical Virology

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Crimean Congo hemorrhagic fever (CCHF) is an acute viral infection that can cause death. The detection of host transcriptome is important for understanding differences in the pathogenesis of the disease. Long noncoding RNAs (lncRNAs) regulate gene expression in different biological processes. They have also emerged as key molecules for therapeutic targets. We investigated the lncRNA gene expression profiles by utilizing the microarray for the first time in CCHF. LncRNAs were determined by the comparisons between case-control, fatal case-control, and fatal case-nonfatal cases. Quantitative polymerase chain reaction was applied to validate the microarray results of some lncRNAs. In our study, 39 lncRNAs (5 downregulated and 34 upregulated) were found to be significantly regulated in the cases when compared to the controls (p < 0.05; FC ≥ 2). One hundred ten lncRNAs exhibited a statistically significant difference between fatal cases and controls. FER1L4, ECRP, and LOC100133669 are important lncRNAs in both case and fatal case groups compared with controls. These lncRNAs may be considered important therapeutic targets for the CCHF in further studies.

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“…Crimean-Congo hemorrhagic fever (CCHF) is the most prevalent tick-borne infection in the world according to notification of the World Health Organization. 1,2 CCHFV belongs to the genus Orthonairovirus of the Nairoviridae family and transported by ticks in the Hyalomma genus. 1,2 Long noncoding RNAs (lncRNAs) play an important role in different levels of gene expression.…”

Section: Introductionmentioning

confidence: 99%

“…1,2 CCHFV belongs to the genus Orthonairovirus of the Nairoviridae family and transported by ticks in the Hyalomma genus. 1,2 Long noncoding RNAs (lncRNAs) play an important role in different levels of gene expression. Important modifications occur in the lncRNA expression of both virus and host genomes.…”

Section: Introductionmentioning

confidence: 99%

Investigation of NEAT1, IFNG‐AS1, and NRIR expression in Crimean–Congo hemorrhagic fever

Bayyurt

Bakır

Engin

et al. 2020

Journal of Medical Virology

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Crimean–Congo hemorrhagic fever (CCHF), whose causative agent is CCHF orthonairovirus (CCHFV), demonstrates different symptoms in patients. Long noncoding RNAs (lncRNAs) take part in various pathological processes of viral diseases. They are prominent regulators of antiviral immune responses. To our knowledge, this study is the first study to investigate nuclear paraspeckle assembly transcript 1 (NEAT1), interferon (IFN) gamma antisense RNA 1 (IFNG‐AS1), and negative regulator of IFN response (NRIR) expression in CCHF in the literature. We selected these lncRNAs because they are related to IFN signal or IFN‐stimulated genes. We investigated NEAT1, IFNG‐AS1, and NRIR gene expression in patients with CCHF. Total RNA was extracted from blood samples of 100 volunteers and NEAT1, IFNG‐AS1, and NRIR expression were measured using a quantitative real‐time polymerase chain reaction. NRIR expression was statistically significant in cases versus controls (p < .001), fatals versus controls (p < .001), and fatals versus nonfatals (p = .01). Furthermore, NRIR was found statistically significant at some clinical parameters including alanine aminotransferase (p = .03), international normalized ratio (p = .03), prothrombin time (p = .02), and active partial thromboplastin time (p = .01) in CCHF cases. NEAT1 and IFNG‐AS1 expression were downregulated in the case and fatal groups which were compared with controls. Our results demonstrate that NRIR may be important in CCHF pathogenesis and the target of CCHF treatment.

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HULC and 7SL RNA expression levels in patients with Crimean‐Congo hemorrhagic fever

Cited by 3 publications

References 24 publications

Do NSm virulence factors in the Bunyavirales viral order originate from Gn gene duplication?

Do NSm virulence factors in the Bunyavirales viral order originate from Gn gene duplication?

Long noncoding RNA expression analysis in Crimean Congo hemorrhagic fever patients

Investigation of NEAT1, IFNG‐AS1, and NRIR expression in Crimean–Congo hemorrhagic fever

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