Human Activin βA Gene IDENTIFICATION OF NOVEL 5′

Tanimoto, Koichi; Yoshida, Eisaku; Mita, Shunji; Nibu, Yutaka; Murakami, Kazuo; Fukamizu, Akiyoshi

doi:10.1074/jbc.271.51.32760

Cited by 48 publications

(12 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is important to recognise that the sampling duration of 72 h and resulting overlap of sampling time-points means that the placement of some events, such as the exact timing of the commencement of the activin A surge and its subsequent decline, are approximations. (Tanimoto et al 1996, Ardekani et al 1998, Yoshida et al 1998. The data also suggest that the reciprocal relationship between the actions of IL-1 and FSH on Sertoli cell, activin A and inhibin B, secretion is modified by the presence of developing spermatogenic cells, consistent with previous observations (Clifton et al 2002).…”

Section: Discussionsupporting

confidence: 80%

Regulated production of activin A and inhibin B throughout the cycle of the seminiferous epithelium in the rat

Okuma

O’Connor

Hayashi

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

Production and regulation of activin A and inhibin B during the cycle of the seminiferous epithelium were investigated in adult rats. Immunohistochemistry localised the activin b Asubunit to the Sertoli cell cytoplasm, with much weaker expression in spermatocytes and spermatids. Both activin A and inhibin B, measured by ELISA were secreted by, seminiferous tubule fragments over 72 h in culture. Activin A was secreted in a cyclic manner with peak secretion from tubules isolated at stage VIII. Tubules collected during stage VI produced the least activin A. Inhibin B secretion was highest from stage IX-I tubules and lowest from stage VII tubules. Addition of interleukin-1b (IL-1b) had relatively little effect on activin A or inhibin B secretion in culture. In contrast, the peak secretion of activin A by stage VIII tubules was blocked by co-incubation with an excess of human recombinant IL-1 receptor antagonist, whereas inhibin B secretion increased slightly. Dibutyryl cAMP stimulated activin A secretion by late stage VII and VIII tubules and stimulated inhibin B across all stages. These data indicate that activin A and inhibin B are cyclically regulated within the seminiferous epithelium, with endogenous IL-1 (presumably IL-1a produced by the Sertoli cells), responsible for a peak of activin A production subsequent to sperm release at stage VIII. These data provide direct evidence that production of activin A and inhibin B by the Sertoli cell is locally modulated by IL-1a, in addition to FSH/cAMP, under the influence of the developing spermatogenic cells.

show abstract

Section: Discussionsupporting

confidence: 80%

Regulated production of activin A and inhibin B throughout the cycle of the seminiferous epithelium in the rat

Okuma

O’Connor

Hayashi

et al. 2006

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Plaque hybridization was performed on a 32p-labelled human activin BA subunit eDNA (14). In the 3 x 106 phages, four positive clones were identified.…”

Section: Methodsmentioning

confidence: 99%

“…Human fibrosarcoma HT1080 cells (ATCC#CCL-121) were transfected with a 5'-deletion construct (5 Ixg), together with pUC19 plasmid (1 Ixg) (-) or with c-Jun expression vector (1 lxg; kindly provided by Prof. Inder M. Verma, The Salk Institute, USA) (+) by calcium phosphate coprecipitation method. After 36 hours of the transfection, cell lysates were prepared and CAT activity was measured as described previously (14). For CAT assay (15), cell extracts (20 to 50 l.tg for transient transfection and 20 ~tg for stable transfection assay) were incubated at 37~ for 1.5 h in 140 mM Tris-C1 (pH 7.8), 0,68 IxCi/ml [14C]chloramphenicol (DuPont), and 580 IxM acetyl coenzyme A (Wako Pure Chemical Industries, Ltd.).…”

Section: Methodsmentioning

confidence: 99%

“…Because activins and inhibins often have opposing effects when present together (13), spatial and temporal regulation of their *To whom correspondence should be addressed. expression of the gene (14). However, to clarify the transcriptional regulation of the activin A gene during development in vivo, it is essential to isolate and identify the 5' regulatory sequences from genes of other species, such as mice.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and characterization of 5′‐regulatory region of mouse activin ßa subunit gene

et al. 1998

Self Cite

View full text Add to dashboard Cite

SUMMARY:We isolated genomic clones that contain the 5'-flanking region of the mouse activin BA subunit gene. The nucleotide sequence determination of the 5'-flanking region of the gene and the comparison of that with the reported mouse cDNA structure identified the putative 5' regulatory region, a novel first exon and a part of the first intron of the gene within this region. The putative 5' regulatory region of the mouse activin BA subunit gene directed the expression of CAT gene in transfected HT 1080 cells. Successive deletions of this region demonstrated a 400-bp region that exerts a strong positive effect on promoter activity of the mouse activin fSA subunit gene.

show abstract

“…The expression of several genes up-regulated in the f-TAF4b SIGCs are known to be activated by c-Jun͞AP-1 ( Table 1). The core promoter regions of the c-jun (13), Ccnd2 (14), Fn1 (15), Fst (16), Inhba (17), , and a 5Ј distal enhancer site within the Vim (19) promoters all possess c-Jun͞AP-1 DNAbinding elements (Fig. 5A).…”

Section: Taf4b and C-jun Promoter Cooccupancy In Gcsmentioning

confidence: 99%

Cell-type-selective induction of c-jun by TAF4b directs ovarian-specific transcription networks

Geles

Freiman

Liu

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Cell-type-selective expression of the TFIID subunit TAFII105 (renamed TAF4b) in the ovary is essential for proper follicle development. Although a multitude of signaling pathways required for folliculogenesis have been identified, downstream transcriptional integrators of these signals remain largely unknown. Here, we show that TAF4b controls the granulosa-cell-specific expression of the proto-oncogene c-jun, and together they regulate transcription of ovary-selective promoters. Instead of using cell-type-specific activators, our findings suggest that the coactivator TAF4b regulates the expression of tissue-specific genes, at least in part, through the cell-type-specific induction of c-jun, a ubiquitous activator. Importantly, the loss of TAF4b in ovarian granulosa cells disrupts cellular morphologies and interactions during follicle growth that likely contribute to the infertility observed in TAF4b-null female mice. These data highlight a mechanism for potentiating tissue-selective functions of the basal transcription machinery and reveal intricate networks of gene expression that orchestrate ovarian-specific functions and cell morphology.ovary ͉ TFIID ͉ short interfering RNA ͉ chromatin immunoprecipitation ͉ granulosa cells S pecific targeting of RNA polymerase (Pol) II to heteromorphic promoters dispersed throughout the genome and packaged into chromatin requires the function of a multitude of individual proteins and multiprotein complexes that collectively direct the modulation of RNA synthesis. At the core of this machinery are the general transcription factors that were first posited to be global in nature and not directly implicated in control of cell-type-specific gene expression. Rather, cell-typespecific expression patterns were largely attributed to DNAbinding activator and repressor proteins that were thought to be expressed in a highly cell-type-specific fashion (1). The general transcription factor IID (TFIID) is a large multiprotein transcription factor that lies at the core of the basal transcription machinery. TFIID is composed of the TATA-box-binding protein (TBP) and a common subset of TBP-associated factors (TAFs) that together perform promoter-selective and coactivator functions during transcription initiation (2). Together, genetic and biochemical studies suggest a conserved and formidable role of TFIID in the execution of global programs of gene expression.

show abstract

Human Activin βA Gene IDENTIFICATION OF NOVEL 5′

Cited by 48 publications

References 60 publications

Regulated production of activin A and inhibin B throughout the cycle of the seminiferous epithelium in the rat

Regulated production of activin A and inhibin B throughout the cycle of the seminiferous epithelium in the rat

Isolation and characterization of 5′‐regulatory region of mouse activin ßa subunit gene

Cell-type-selective induction of c-jun by TAF4b directs ovarian-specific transcription networks

Contact Info

Product

Resources

About